NDLI: Interaction of Cm(III) and Am(III) with human serum transferrin studied by time-resolved laser fluorescence and EXAFS spectroscopy

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bauer, Nicole Fröhlich, Daniel R. Panak, Petra J.
Description	Author Affiliation: Bauer N ( Karlsruhe Institute of Technology (KIT), Campus North, Institute for Nuclear Waste Disposal (INE), P.O. Box 3640, 76021 Karlsruhe, Germany. Nicole.bauer@kit.edu.)
Abstract	The complexation of Cm(III) with human serum transferrin was investigated in a pH range from 3.5 to 11.0 using time-resolved laser fluorescence spectroscopy (TRLFS). At pH ≥ 7.4 Cm(III) is incorporated at the Fe(III) binding site of transferrin whereas at lower pH a partially bound Cm(III) transferrin species is formed. At physiological temperature (310 K) at pH 7.4, about 70% of the partially bound and 30% of the incorporated Cm(III) transferrin species are present in solution. The Cm(III) results obtained by TRLFS are in very good agreement with Am(III) EXAFS results, confirming the incorporation of Am(III) at the Fe(III) binding site at pH 8.5.
ISSN	14779226
Issue Number	18
Volume Number	43
e-ISSN	13645447
Journal	Dalton Trans.
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2014-05-14
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Fluoroimmunoassay Transferrin Analysis Metabolism X-Ray Absorption Spectroscopy Curium Methods Humans Protein Binding Physiology Journal Article Chemistry
Content Type	Text
Resource Type	Article
Subject	Inorganic Chemistry

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Interaction of Cm(III) and Am(III) with human serum transferrin studied by time-resolved laser fluorescence and EXAFS spectroscopy