NDLI: $Ni^{2+}-zeolite/ferrosphere$ and $Ni^{2+}-silica/ferrosphere$ beads for magnetic affinity separation of histidine-tagged proteins

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Frank, L. A. Kudryavtsev, A. N. Anshits, A. G. Sharonova, O. M. Fedorchak, M. A. Zhizhaev, A. M. Fomenko, E. V. Shishkina, N. N. Vereshchagina, T. A.
Description	Author Affiliation: Vereshchagina TA ( Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, 660036, Russia. tatiana_ver@mail.ru.); Fedorchak MA ( Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, 660036, Russia. tatiana_ver@mail.ru.); Sharonova OM ( Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, 660036, Russia. tatiana_ver@mail.ru.); Fomenko EV ( Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, 660036, Russia. tatiana_ver@mail.ru.); Shishkina NN ( Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, 660036, Russia. tatiana_ver@mail.ru.); Zhizhaev AM ( Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, 660036, Russia. tatiana_ver@mail.ru.); Kudryavtsev AN ( Institute of Biophysics SB RAS, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia. lfrank@yandex.ru.); Frank LA ( Institute of Biophysics SB RAS, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia. lfrank@yandex.ru and Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk, 660041, Russia. anshits@icct.ru.); Anshits AG ( Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, 660036, Russia. tatiana_ver@mail.ru and Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk, 660041, Russia. anshits@icct.ru.)
Abstract	Magnetic $Ni^{2+}-zeolite/ferrosphere$ and $Ni^{2+}-silica/ferrosphere$ beads (Ni-ferrosphere beads – NFB) of a core–shell structure were synthesized starting from coal fly ash ferrospheres having diameters in the range of 0.063–0.050 mm. The strategy of NFB fabrication is an oriented chemical modification of the outer surface preserving the magnetic core of parent beads with the formation of micro–mesoporous coverings. Two routes of ferrosphere modification were realized, such as (i) hydrothermal treatment in an alkaline medium resulting in a NaP zeolite layer and (ii) synthesis of micro–mesoporous silica on the glass surface using conventional methods. Immobilization of $Ni^{2+}$ ions in the siliceous porous shell of the magnetic beads was carried out via (i) the ion exchange of $Na^{+}$ for $Ni^{2+}$ in the zeolite layer or (ii) deposition of NiO clusters in the zeolite and silica pores. The final NFB were tested for affinity in magnetic separation of the histidine-tagged green fluorescent protein (GFP) directly from a cell lysate. Results pointed to the high affinity of the magnetic beads towards the protein in the presence of 10 mM EDTA. The sorption capacity of the ferrosphere-based Ni-beads with respect to GFP was in the range 1.5–5.7 mg $cm^{−3}.$
ISSN	14779226
Issue Number	4
Volume Number	45
e-ISSN	13645447
Journal	Dalton Trans.
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2016-01-28
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Green Fluorescent Proteins Isolation & Purification Histidine Chemistry Iron Compounds Nickel Organometallic Compounds Silicon Dioxide Zeolites Animals Escherichia Coli Cytology Magnetic Phenomena Chemical Synthesis Particle Size Porosity Scyphozoa Surface Properties Journal Article Research Support, Non-U.S. Gov't
Content Type	Text
Resource Type	Article
Subject	Inorganic Chemistry

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