NDLI: Discrimination of G-Quadruplexes from Duplex and Single-Stranded DNAs with Fluorescence and Energy-Transfer Fluorescence Spectra of Crystal Violet

Please wait, while we are loading the content...

Year: 2017 Volume: 23

Year: 2016 Volume: 22

Year: 2015 Volume: 21

Year: 2014 Volume: 20

Year: 2013 Volume: 19

Year: 2012 Volume: 18

Year: 2011 Volume: 17

Year: 2010 Volume: 16

Year: 2009 Volume: 15

Year: 2009 Volume: 15 Issue: 48

Year: 2009 Volume: 15 Issue: 47

Year: 2009 Volume: 15 Issue: 46

Year: 2009 Volume: 15 Issue: 45

Year: 2009 Volume: 15 Issue: 44

Year: 2009 Volume: 15 Issue: 43

Year: 2009 Volume: 15 Issue: 42

Year: 2009 Volume: 15 Issue: 41

Year: 2009 Volume: 15 Issue: 40

Year: 2009 Volume: 15 Issue: 39

Year: 2009 Volume: 15 Issue: 38

Year: 2009 Volume: 15 Issue: 37

Year: 2009 Volume: 15 Issue: 36

Year: 2009 Volume: 15 Issue: 35

Year: 2009 Volume: 15 Issue: 34

Year: 2009 Volume: 15 Issue: 33

Year: 2009 Volume: 15 Issue: 32

Year: 2009 Volume: 15 Issue: 31

Year: 2009 Volume: 15 Issue: 30

Year: 2009 Volume: 15 Issue: 29

Year: 2009 Volume: 15 Issue: 28

Year: 2009 Volume: 15 Issue: 27

Year: 2009 Volume: 15 Issue: 26

Year: 2009 Volume: 15 Issue: 25

Year: 2009 Volume: 15 Issue: 24

Year: 2009 Volume: 15 Issue: 23

Year: 2009 Volume: 15 Issue: 22

Year: 2009 Volume: 15 Issue: 21

Year: 2009 Volume: 15 Issue: 20

Year: 2009 Volume: 15 Issue: 19

Year: 2009 Volume: 15 Issue: 18

Year: 2009 Volume: 15 Issue: 17

Year: 2009 Volume: 15 Issue: 16

Year: 2009 Volume: 15 Issue: 15

Year: 2009 Volume: 15 Issue: 14

Year: 2009 Volume: 15 Issue: 13

Year: 2009 Volume: 15 Issue: 12

Year: 2009 Volume: 15 Issue: 11

Year: 2009 Volume: 15 Issue: 10

Year: 2009 Volume: 15 Issue: 9

Year: 2009 Volume: 15 Issue: 8

Year: 2009 Volume: 15 Issue: 7

Year: 2009 Volume: 15 Issue: 6

Year: 2009 Volume: 15 Issue: 5

Year: 2009 Volume: 15 Issue: 4

Multifunctional G-Quadruplex Aptamers and Their Application to Protein Detection

Discrimination of G-Quadruplexes from Duplex and Single-Stranded DNAs with Fluorescence and Energy-Transfer Fluorescence Spectra of Crystal Violet

Construction of Fluorescent Probes Via Protection/Deprotection of Functional Groups: A Ratiometric Fluorescent Probe for $Cu^{2+}$

Metal Nitride Cluster Fullerene $M_{3}N@C_{80}$ (M=Y, Sc) Based Dyads: Synthesis, and Electrochemical, Theoretical and Photophysical Studies

Total Synthesis of (+)-Neomarinone

An Unusual Stable Mononuclear $Mn^{III}$ Bis-terpyridine Complex Exhibiting Jahn–Teller Compression: Electrochemical Synthesis, Physical Characterisation and Theoretical Study

Effects on the Reactivity by Changing the Electrophilic Center from CO to CS: Contrasting Reactivity of Hydroxide, p-Chlorophenoxide, and Butan-2,3-dione Monoximate in $DMSO/H_{2}O$ Mixtures

Luminescent Bimetallic Lanthanide Bioprobes for Cellular Imaging with Excitation in the Visible-Light Range

Structural Analysis of Zincocenes with Substituted Cyclopentadienyl Rings

Synthesis and Characterization of 2-Mono- and 1,2-Diaminocarba-closo-dodecaborates $M[1-R-2-H_{2}N-closo-CB_{11}H_{10}]$ (R=H, Ph, $H_{2}N,$ $CyHN)^{†}$

Lewis Acid or Brønsted Acid Catalyzed Reactions of Vinylidene Cyclopropanes with Activated Carbon–Nitrogen, Nitrogen–Nitrogen, and Iodine–Nitrogen Double-Bond-Containing Compounds

Rainbow Perylene Monoimides: Easy Control of Optical Properties

Modular Synthesis of Tetrasubstituted Imidazoles and Trisubstituted Oxazoles by Aldimine Cross-Coupling

High-Temperature Chlorination-Reduction Sequence for the Preparation of Silicon Hydride Modified Silica $Surfaces^{†}$

Highly Dense Nitranilates-Containing Nitrogen-Rich Cations

A highly active heterogeneous palladium catalyst supported on a synthetic adsorbent.

Indenyl Abstraction versus Alkyl Abstraction of $[(Indenyl)ScR_{2}(thf)]$ by $[Ph_{3}C]$ $[B(C_{6}F_{5})_{4}]:$ Aspecific and Syndiospecific Styrene Polymerization

Heterogeneous Versus Homogeneous Palladium Catalysts for Ligandless Mizoroki–Heck Reactions: A Comparison of Batch/Microwave and Continuous-Flow Processing

From Conjugated Tertiary Skipped Diynes to Chain-Functionalized Tetrasubstituted Pyrroles

Intermolecular Olefin Functionalisation Involving Aryl Radicals Generated from Arenediazonium Salts

Enantioselective Synthesis of Functionalized Nitrocyclopropanes by Organocatalytic Conjugate Addition of Bromonitroalkanes to α,β-Unsaturated Enones

Synthesis of Structurally Well-Defined Telechelic Polymers by Organostibine-Mediated Living Radical Polymerization: In Situ Generation of Functionalized Chain-Transfer Agents and Selective ω-End-Group Transformations

Breaking the Mirror: pH-Controlled Chirality Generation from a meso Ligand to a Racemic Ligand

Electron Donation in the Water–Water Hydrogen Bond

Structural and Magnetic Insights into the Trinuclear Ferrocenophane and Unexpected Hydrido Inverse Crown Products of Alkali-Metal-Mediated Manganation(II) of Ferrocene

Year: 2009 Volume: 15 Issue: 3

Year: 2009 Volume: 15 Issue: 2

Year: 2009 Volume: 15 Issue: 1

Year: 2008 Volume: 14

Year: 2007 Volume: 13

Year: 2006 Volume: 12

Year: 2005 Volume: 11

Year: 1997 Volume: 3

Discrimination of G-Quadruplexes from Duplex and Single-Stranded DNAs with Fluorescence and Energy-Transfer Fluorescence Spectra of Crystal Violet

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ma, Yong-e Wu, Jing Kong, De-ming Shen, Han-xi
Description	Author Affiliation: Kong DM ( Key Laboratory of Functional Polymer Materials, Ministry of Education, Nankai University, Tianjin, China. kongdem@nankai.edu.cn)
Abstract	G-rich nucleic acid sequences with the potential to form G-quadruplex structures are common in biologically important regions. Most of these sequences are present with their complementary strands, so the development of a sensitive biosensor to distinguish G-quadruplex and duplex structures and to determine the competitive ability of quadruplex to duplex structures has received a great deal of attention. In this work, the interactions between two triphenylmethane dyes (malachite green (MG) and crystal violet (CV)) and G-quadruplex, duplex, or single-stranded DNAs were studied by fluorescence spectroscopy and energy-transfer fluorescence spectroscopy. Good discrimination between quadruplexes and duplex or single-stranded DNAs can be achieved by using the fluorescence spectrum of CV or the energy-transfer fluorescence spectra of CV and MG. In addition, by using energy-transfer fluorescence titrations of CV with G-quadruplexes, the binding-stoichiometry ratios of CV to G-quadruplexes can be determined. By using the fluorescence titrations of G-quadruplex–CV complexes with C-rich complementary strands, the fraction of G-rich oligonucleotide that engages in G-quadruplex structures in the presence of the complementary sequence can be measured. This study may provide a simple method for discrimination between quadruplexes and duplex or single-stranded DNAs and for measuring G-quadruplex percentages in the presence of the complementary C-rich sequences.
ISSN	09476539
e-ISSN	15213765
Journal	Chemistry - A European Journal
Issue Number	4
Volume Number	15
Language	English
Publisher	Wiley-VCH;ChemPubSoc Europe
Publisher Date	2009-01-01
Publisher Place	Germany
Access Restriction	Open
Subject Keyword	DNA, Single-Stranded Chemistry DNA Fluorescent Dyes G-Quadruplexes Gentian Violet Circular Dichroism Energy Transfer Radiation Effects Oligonucleotides Spectrometry, Fluorescence Research Support, Non-U.S. Gov't
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Catalysis

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in