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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Nishizawa, Seiichi Sato, Yusuke Nakamura, Kodai Seino, Takehiro Teramae, Norio Zhang, Yushuang Li, Minjie |
| Description | Author Affiliation: Sato Y ( Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai, Japan.) |
| Abstract | A fluorescence assay for theophylline, one of the common drugs for acute and chronic asthmatic conditions, has been developed based on an abasic site-containing DNA duplex aptamer (AP aptamer) in combination with an abasic site-binding fluorescent ligand, riboflavin. The assay is based on the competitive binding of theophylline and riboflavin at the abasic (AP) site of the AP aptamer. In the absence of theophylline, riboflavin binds to the receptor nucleotide opposite the AP site, which leads to fluorescence quenching of the riboflavin. Upon addition of theophylline, competitive binding occurs between theophylline and riboflavin, which results in an effective fluorescence restoration due to release of riboflavin from the AP site. From an examination of the optimization of the AP aptamers, the complex of riboflavin with a 23-mer AP aptamer (5′-TCT GCG TCC AGX GCA ACG CAC AC-3′/5′-GTG TGC GTT GCC CTG GAC GCA GA-3′; X: the AP site (Spacer C3, a propylene residue)) possessing cytosine as a receptor nucleotide was found to show a selective and effective fluorescence response to theophylline; the limit of detection for theophylline was 1.1 μM. Furthermore, fluorescence detection of theophylline was successfully demonstrated with high selectivity in serum samples by using the optimized AP aptamer and riboflavin. |
| ISSN | 09476539 |
| e-ISSN | 15213765 |
| Journal | Chemistry - A European Journal |
| Issue Number | 40 |
| Volume Number | 18 |
| Language | English |
| Publisher | Wiley-VCH;ChemPubSoc Europe |
| Publisher Date | 2012-10-01 |
| Publisher Place | Germany |
| Access Restriction | Open |
| Subject Keyword | Aptamers, Nucleotide Chemistry Cytosine DNA Nucleotides Riboflavin Theophylline Blood Binding Sites Fluorescence Ligands Molecular Sequence Data Polymorphism, Single Nucleotide Spectrometry, Fluorescence Research Support, Non-U.S. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Catalysis |
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