NDLI: Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost-Effective Diagnosis of Infectious Disease

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Charles, Richelle C. Ryan, Edward T. Lee, Hakho Park, Ki Soo Weissleder, Ralph
Description	Author Affiliation: Park KS ( Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 (USA).); Charles RC ( Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114 (USA).); Ryan ET ( Department of Medicine, Harvard Medical School, Boston, MA 02114 (USA).); Weissleder R ( Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114 (USA).); Lee H ( Department of Medicine, Harvard Medical School, Boston, MA 02114 (USA).)
Abstract	A new nucleic acid detection method was developed for a rapid and cost-effective diagnosis of infectious disease. This approach relies on the three unique elements: 1) detection probes that regulate DNA polymerase activity in response to the complementary target DNA; 2) universal reporters conjugated with a single fluorophore; and 3) fluorescence polarization (FP) detection. As a proof-of-concept, the assay was used to detect and sub-type Salmonella bacteria with sensitivities down to a single bacterium in less than three hours.
ISSN	09476539
e-ISSN	15213765
Journal	Chemistry - A European Journal
Issue Number	46
Volume Number	21
Language	English
Publisher	Wiley-VCH;ChemPubSoc Europe
Publisher Date	2015-11-09
Publisher Place	Germany
Access Restriction	Open
Subject Keyword	Biosensing Techniques Communicable Diseases Diagnosis DNA-Directed DNA Polymerase Chemistry Fluorescent Dyes Nucleic Acid Amplification Techniques Nucleic Acids Salmonella Cost-Benefit Analysis Metabolism Fluorescence Polarization Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Catalysis

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