Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Evans, C. A. Snell, K. |
| Description | Author Affiliation: Snell K ( Department of Biochemistry, University of Surrey, Guildford.) |
| Abstract | 1. The subtype specificity of beta-adrenoceptors in foetal (20 days post coitum) rat liver membrane preparations has been determined by use of [125I]-iodopindolol binding assays and the characteristics of radioligand binding have been resolved. 2. The kinetics of radioligand association and dissociation (in the presence of 5 x 10(-4) M isoprenaline) showed an association rate constant of 1.5 x 10(7) M-1 S-1 and dissociation rate constant of 9.1 x 10(-4) S-1, corresponding to a dissociation constant for [125I]-iodopindolol of 60.7 pM. A similar dissociation constant (75 pM) was determined by saturation binding assays. 3. The rank order of potency for displacement of [125I]-iodopindolol binding was consistent with binding to a predominantly beta 2-adrenoceptor population (i.e. ICI 118551 greater than isoprenaline greater than adrenaline greater than noradrenaline greater than atenolol). Computer analysis of displacement curves in the presence of a beta 1-subtype selective agent (atenolol) or a beta 2-subtype selective agent (ICI 118551) revealed the presence of beta 2- and beta 1-adrenoceptor subtypes in a ratio of about 80:20%. 4. Saturation binding assays by use of [125I]-iodopindolol were carried out at different perinatal ages to determine total beta-adrenoceptor concentrations and beta 2-subtype (in the presence of 5 x 10(-7) M atenolol) adrenoceptor concentrations. Competition binding assays with atenolol confirmed that at all ages apparent beta 2-adrenoceptor binding accounted for 84-95% of the total beta-adrenoceptor binding. The total beta- and beta 2-adrenoceptor binding capacity increased by 2.3 fold from 20 days post coitum to birth, and then decreased postnatally at 1 and 2 days post partum. The dissociation constant for [125I]-iodopindolol binding did not show any change with age. 5. The change in beta 2-adrenoceptor concentration with age is discussed in relation to the changing beta-adrenoceptor-mediated responsiveness of glucose production by rat liver during perinatal development. |
| ISSN | 00071188 |
| e-ISSN | 14765381 |
| Journal | British Journal of Pharmacology |
| Issue Number | 4 |
| Volume Number | 93 |
| Language | English |
| Publisher | Wiley Online Library(on behalf of The British Pharmacological Society) |
| Publisher Date | 1988-04-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Open |
| Subject Keyword | Liver Metabolism Pindolol Analogs & Derivatives Receptors, Adrenergic, Beta Animals Cell Membrane Drug Effects Iodine Radioisotopes Kinetics Embryology Pregnancy Radioligand Assay Rats, Inbred Strains Research Support, Non-U.S. Gov't Pharmacology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|