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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kim, S. Sapkota, K. Kim, S. J. Kim, H. Park, S. E. |
| Description | Author Affiliation: Park SE ( Department of Biotechnology, Chosun University, Gwangju, Korea.) |
| Abstract | BACKGROUND AND PURPOSE: Kaempferol, a dietary flavonoid and phyto-oestrogen, is known to have anti-inflammatory properties. Microglial activation has been implicated in various neurodegenerative diseases. Anti-inflammatory effects of kaempferol and the underlying mechanisms were investigated by using LPS-stimulated microglial BV2 cells. EXPERIMENTAL APPROACH: Cell viability was measured using MTT and neutral red assays. elisa, Western blot, immunocytochemistry and electrophoretic mobility-shift assay were used to analyse NO, PGE(2) , TNF- and IL-1ß production, inducible NOS (iNOS), COX-2 expression and the involvement of signalling pathways such as toll-like receptor-4 (TLR4), MAPK cascades, PKB (AKT) and NF-κB. Accumulation of reaction oxygen species (ROS) was measured by nitroblue tetrazolium and 2'7'-dichlorofluorescein diacetate assay. Matrix metalloproteinase activity was investigated by zymography and immunoblot assay. Phagocytotic activity was assessed by use of latex beads. KEY RESULTS: Kaempferol significantly attenuated LPS-induced NO, PGE(2) , TNF- , IL-1ß and ROS production and phagocytosis in a concentration-dependent manner. Kaempferol suppressed the expression of iNOS, COX-2, MMP-3 and blocked the TLR4 activation. Moreover, kaempferol inhibited LPS-induced NF-κB activation and p38 MAPK, JNK and AKT phosphorylation. CONCLUSION AND IMPLICATIONS: Kaempferol was able to reduce LPS-induced inflammatory mediators through the down-regulation of TLR4, NF-κB, p38 MAPK, JNK and AKT suggesting that kaempferol has therapeutic potential for the treatment of neuroinflammatory diseases. |
| ISSN | 00071188 |
| e-ISSN | 14765381 |
| Journal | British Journal of Pharmacology |
| Issue Number | 3 |
| Volume Number | 164 |
| Language | English |
| Publisher | Wiley Online Library(on behalf of The British Pharmacological Society) |
| Publisher Date | 2011-10-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Open |
| Subject Keyword | Kaempferols Pharmacology Microglia Drug Effects Metabolism Mitogen-Activated Protein Kinase Kinases Proto-Oncogene Proteins C-akt Antagonists & Inhibitors Animals Anti-Inflammatory Agents Cell Survival Cells, Cultured Cyclooxygenase 2 Genetics Dinoprostone Biosynthesis Interleukin-1beta Lipopolysaccharides Matrix Metalloproteinase 3 Matrix Metalloproteinase Inhibitors Mice Enzymology NF-kappa B Nitric Oxide Nitric Oxide Synthase Type II Phagocytosis Phosphorylation Protein Binding Reactive Oxygen Species Signal Transduction Toll-Like Receptor 4 Tumor Necrosis Factor-alpha P38 Mitogen-Activated Protein Kinases Research Support, Non-U.S. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology |
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