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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Guimarães Pereira, Gonçalo Amarante Ramos Dos Santos, Camila De Souza Dantas, Deyse Medrano, Francisco Javier |
| Description | Author Affiliation: de Souza Dantas D ( Laboratorio de Genômica e Expressão, Departamento de Genética e Evolução, IB-UNICAMP, Caixa Postal 6109, CEP 13083-970, Campinas, Brazil.) |
| Abstract | The 6-oxopurine phosphoribosyltransferase (HPRT, EC 2.4.2.8) from the hyperthermophile Pyrococcus horikoshii was expressed in Escherichia coli and purified. Steady-state kinetic studies indicated that the enzyme is able to use hypoxanthine, guanine and xanthine. The first two substrates showed similar catalytic efficiencies, and xanthine presented a much lower value (around 20 times lower), but the catalytic constant was comparable to that of hypoxanthine. The enzyme was not able to bind to GMP-agarose, but was able to bind the other reverse reaction substrate, inorganic pyrophosphate, with low affinity ( $K_{d}$ of 4.7 ± 0.1 mM). Dynamic light scattering and analytical gel filtration suggested that the enzyme exists as a homohexamer in solution. |
| ISSN | 00063002 |
| Journal | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer |
| Issue Number | 6 |
| Volume Number | 1784 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2008-06-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Archaeal Proteins Metabolism Pentosyltransferases Pyrococcus Horikoshii Enzymology Amino Acid Sequence Chemistry Genetics Chromatography, Gel Circular Dichroism Dimerization Electrophoresis, Polyacrylamide Gel Guanine Guanosine Monophosphate Hypoxanthine Molecular Sequence Data Sequence Homology, Amino Acid Xanthine Research Support, Non-U.S. Gov't Biochemistry |
| Content Type | Text |
| Resource Type | Article |
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