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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Dempski, Robert E. Friedrich, Thomas Bamberg, Ernst |
| Description | Author Affiliation: Dempski RE ( Max-Planck-Institute of Biophysics, Department of Biophysical Chemistry, Max-von-Laue-Strasse 3, D-60438 Frankfurt am Main, Germany.) |
| Abstract | This paper summarizes our recent work investigating the conformational dynamics and structural arrangement of the $Na^{+}/K^{+}-ATPase$ using voltage clamp fluorometry as well as the latest biochemical, biophysical and structural results from other laboratories. Our research has been focused on combining site-specific fluorophore labeling on the alpha, beta and/or gamma subunit with electrophysiological studies to investigate partial reactions of the ion pump by monitoring changes in fluorescence intensity following voltage pulses and/or solution exchange. As a consequence of these studies, we have been able to identify a residue on the beta subunit, which following labeling with tetramethylrhodamine-6-maleimide can be used as a reporter group to monitor the conformational state of the holoenzyme. Furthermore, we have been able to delineate distance constraints between the alpha, beta and gamma subunits and to examine the relative movements of these proteins during ion transport. Concurrent to this research, significant advancements have been made in understanding the molecular mechanism of the $Na^{+}/K^{+}-ATPase.$ Thus, our research will be compared with the results from other groups and future experimental directions will be proposed. |
| ISSN | 00063002 |
| Journal | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer |
| Issue Number | 6 |
| Volume Number | 1787 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2009-06-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Fluorometry Sodium-Potassium-Exchanging ATPase Chemistry Metabolism Animals Crystallography, X-Ray Electrophysiological Phenomena Fluorescence In Vitro Techniques Models, Biological Models, Molecular Mutagenesis, Site-Directed Oocytes Patch-Clamp Techniques Recombinant Proteins Genetics Xenopus Research Support, Non-U.S. Gov't Biochemistry |
| Content Type | Text |
| Resource Type | Article |
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