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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Tullio, Francesca Moro, Francesca Mancardi, Daniele Pagliaro, Pasquale Perrelli, Maria-giulia Raimondo, Stefania Merlino, Annalisa Penna, Claudia Geuna, Stefano |
| Description | Author Affiliation: Penna C ( Department of Clinical and Biological Sciences, University of Turin, Italy.) |
| Abstract | Postconditioning (PostC) may limit mitochondrial damage and apoptotic signaling. We studied markers of apoptosis and mitochondrial protection in isolated rat hearts, which underwent a ) perfusion without ischemia (Sham), b ) 30-min ischemia (I) plus 2-hour reperfusion (R), or c ) PostC protocol (5 intermittent cycles of 10-s reperfusion and 10-s ischemia immediately after the 30-min ischemia). Markers were studied in cytosolic (CF) and/or mitochondrial (MF) fractions. In CF, while pro-apoptotic factors (cytochrome c and caspase-3) were reduced, the anti-apoptotic markers (Bcl-2 and Pim-1) were increased by PostC, compared to the I/R group. Accordingly, phospho-GSK-3β and Bcl-2 levels increased in mitochondria of PostC group. Moreover, I/R reduced the level of mitochondrial structural protein (HSP-60) in MF and increased in CF, thus suggesting mitochondrial damage and HSP-60 release in cytosol, which were prevented by PostC. Electron microscopy confirmed that I/R markedly damaged cristae and mitochondrial membranes; damage was markedly reduced by PostC. Finally, total connexin-43 (Cx43) levels were reduced in the CF of the I/R group, whereas phospho-Cx43 level resulted in higher levels in the MF of the I/R group than the Sham group. PostC limited the I/R-induced increase of mitochondrial phospho-Cx43. Data suggest that PostC i ) increases the levels of anti-apoptotic markers, including the cardioprotective kinase Pim-1, ii ) decreases the pro-apoptotic markers, e.g. cytochrome c , iii ) preserves the mitochondrial structure, and iv) limits the migration of phospho-Cx43 to mitochondria. |
| ISSN | 00063002 |
| Journal | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer |
| Issue Number | 7 |
| Volume Number | 1787 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2009-07-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Apoptosis Ischemic Preconditioning, Myocardial Mitochondria, Heart Metabolism Myocardial Reperfusion Injury Therapy Myocardium Animals Caspase 3 Chaperonin 60 Connexin 43 Cytochromes c Cytosol Glycogen Synthase Kinase 3 Enzymology Ultrastructure Mitochondrial Membranes Perfusion Phosphorylation Proto-Oncogene Proteins C-bcl-2 Proto-Oncogene Proteins C-pim-1 Random Allocation Rats, Wistar Research Support, Non-U.S. Gov't Biochemistry |
| Content Type | Text |
| Resource Type | Article |
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