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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ausili, Alessio Corbalán-garcía, Senena De Godos, Ana Gómez-fernández, Juan C. Torrecillas, Alejandro |
| Description | Author Affiliation: Ausili A ( Departamento de Bioquímica y Biología Molecular (A), Facultad de Veterinaria, Universidad de Murcia, Apartado de Correos 4021, E-30080-Murcia, Spain.) |
| Abstract | The C-terminal domain of the pro-apoptotic protein Bax (Bax-C) is supposed to act as a membrane anchor motif when Bax is activated leading to programmed cell death. A synthetic peptide which imitates this domain has been used to study the mechanism of peptide–phospholipid interaction. We have used static and MAS-NMR techniques to show that the interaction of Bax-C with membranes is modulated by the presence of a negatively charged phospholipid like phosphatidylglycerol. Bax-C slightly shifted upfield the $^{31}P$ resonances coming from phosphatidylglycerol and phosphatidylcholine. However the width of the resonance peaks was considerably higher when phosphatidylglycerol was present. Bax-C substantially decreased the $T_{1}$ relaxation times of phosphatidylglycerol and those of phosphatidylcholine when mixtured with phosphatidylglycerol, but $T_{1}$ values were not decreased when phosphatidylcholine was the only phospholipid present in the membrane. $^{13}C-MAS-NMR$ showed that $T_{1}$ values were decreased when Bax-C was incorporated into the lipid vesicles and this reduction affected similarly to carbons located in different regions of the membrane when the only phospholipid present was phosphatidylcholine. However, when phosphatidylglycerol was also present, the decrease in $T_{1}$ affected considerably more to some carbons in the polar region. These results indicate that Bax-C interacts differently with the polar part of the membrane depending on whether phosphatidylglycerol is present or not, suggesting that an electrostatic interaction of Bax-C with the membrane determines the location of this domain. Fluorescence spectroscopy showed that the Trp residues of Bax-C were placed in a microenvironment more hydrophobic and less accessible to quenching by acrylamide when phosphatidylglycerol was present. |
| ISSN | 00063002 |
| Journal | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer |
| Issue Number | 9 |
| Volume Number | 1788 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2009-09-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Phospholipids Metabolism Protein Structure, Tertiary Bcl-2-Associated X Protein Carbon Isotopes Nuclear Magnetic Resonance, Biomolecular Phosphatidylcholines Chemistry Phosphatidylglycerols Spectrometry, Fluorescence Biochemistry |
| Content Type | Text |
| Resource Type | Article |
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