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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bortolotti, Ana Medina, Milagros Pérez-dorado, Inmaculada Hermoso, Juan A. Cortez, Néstor Goñi, Guillermina Carrillo, Néstor |
| Description | Author Affiliation: Bortolotti A ( Instituto de Biología Molecular y Celular de Rosario, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina.) |
| Abstract | Ferredoxin-NADP(H) reductases catalyse the reversible hydride/electron exchange between NADP(H) and ferredoxin/flavodoxin, comprising a structurally defined family of flavoenzymes with two distinct subclasses. Those present in Gram-negative bacteria (FPRs) display turnover numbers of 1–5 s $^{−$ 1 while the homologues of cyanobacteria and plants (FNRs) developed a 100-fold activity increase. We investigated nucleotide interactions and hydride transfer in Rhodobacter capsulatus FPR comparing them to those reported for FNRs. NADP(H) binding proceeds as in FNRs with stacking of the nicotinamide on the flavin, which resulted in formation of charge-transfer complexes prior to hydride exchange. The affinity of FPR for both NADP(H) and 2′-P-AMP was 100-fold lower than that of FNRs. The crystal structure of FPR in complex with 2′-P-AMP and NADP $^{+}$ allowed modelling of the adenosine ring system bound to the protein, whereas the nicotinamide portion was either not visible or protruding toward solvent in different obtained crystals. Stabilising contacts with the active site residues are different in the two reductase classes. We conclude that evolution to higher activities in FNRs was partially favoured by modification of NADP(H) binding in the initial complexes through changes in the active site residues involved in stabilisation of the adenosine portion of the nucleotide and in the mobile C-terminus of FPR. |
| ISSN | 00063002 |
| Journal | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer |
| Issue Number | 2 |
| Volume Number | 1794 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2009-02-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Bacterial Proteins Chemistry Ferredoxin-NADP Reductase Ferredoxins Metabolism Flavodoxin Rhodobacter Capsulatus Enzymology Adenosine Adenosine Diphosphate Catalytic Domain Crystallography, X-Ray Kinetics Models, Molecular NADP Protein Binding Protein Conformation Recombinant Proteins Research Support, Non-U.S. Gov't Biochemistry |
| Content Type | Text |
| Resource Type | Article |
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