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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Marsellach, Francesc-xavier Huertas, Dori Azorín, Fernando Batlle, Marta |
| Description | Author Affiliation: Batlle M ( Institute of Molecular Biology of Barcelona, CSIC, Institute for Research in Biomedicine (IRB Barcelona), Barcelona Science Park, Baldiri Rexac, 12, 08028 Barcelona, Spain.) |
| Abstract | Functional characterisation of vigilin, a highly conserved multi-KH-domain protein that binds RNA and ssDNA, remains elusive and, to some extent, controversial. Studies performed in Saccharomyces cerevisiae and human cells indicate that vigilin localises to the cytoplasm, binds ribosomes, associates to RER and regulates mRNA translation. On the other hand, we and others reported a contribution to heterochromatin-mediated gene silencing (PEV) and chromosome segregation in S. cerevisiae, Drosophila and human cells. Whether this contribution is direct remains, however, unclear. Here, we report that Drosophila vigilin, DDP1, vastly localises to the cytoplasm, being largely excluded from the nucleus. We also show that DDP1 preferentially associates to RER and co-purifies with several ribosomal proteins, suggesting a contribution to mRNA translation. In light of these results, the contribution of DDP1 to PEV was re-examined. Here, we show that a newly generated null $ddp1^{Δ}$ mutation is only a weak suppressor of PEV, which is in contrast with our own previous results showing dominant suppression in the presence of a strong hypomorphic $ddp1^{15.1}$ mutation. Similar results were obtained in the fission yeast Schizosaccharomyces pombe, where vigilin (Vgl1) also associates to RER, having no significant contribution to PEV at centromeres, telomeres and the mating-type locus. Altogether, these results indicate that cytoplasmic localisation and association to RER, but not contribution to heterochromatin organisation, are evolutionarily conserved features of vigilin, favouring a model by which vigilin acts in the cytoplasm, regulating RNA metabolism, and affects nuclear functions only indirectly. |
| ISSN | 00063002 |
| Journal | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer |
| Issue Number | 1 |
| Volume Number | 1809 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-01-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | DNA-Binding Proteins Metabolism Drosophila Proteins Drosophila Melanogaster Endoplasmic Reticulum, Rough Animals Animals, Genetically Modified Blotting, Western Cells, Cultured Cytoplasm Genetics Cytology Embryo, Nonmammalian Embryology Ultrastructure Green Fluorescent Proteins Heterochromatin Histones Immunohistochemistry Lysine Methylation Microscopy, Immunoelectron Mutation Protein Binding RNA Interference RNA-Binding Proteins Ribosomal Proteins Schizosaccharomyces Schizosaccharomyces Pombe Proteins Research Support, Non-U.S. Gov't Biochemistry |
| Content Type | Text |
| Resource Type | Article |
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