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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Gregor, Ingo Enderlein, Jörg Busch, Karin B. Söhnel, Anna-carina Kohl, Wladislaw Rieger, Bettina |
| Description | Author Affiliation: Söhnel AC ( Mitochondrial Dynamics, School of Biology, University of Osnabrück, D-49076 Osnabrück, Germany); Kohl W ( Mitochondrial Dynamics, School of Biology, University of Osnabrück, D-49076 Osnabrück, Germany.); Gregor I ( Physics Department III, University of Göttingen, D-37077 Göttingen, Germany.); Enderlein J ( Physics Department III, University of Göttingen, D-37077 Göttingen, Germany.); Rieger B ( Mitochondrial Dynamics, School of Biology, University of Osnabrück, D-49076 Osnabrück, Germany); Busch KB ( Mitochondrial Dynamics, School of Biology, University of Osnabrück, D-49076 Osnabrück, Germany) |
| Abstract | The cell is metabolically highly compartmentalized. Especially, mitochondria host many vital reactions in their different microcompartments. However, due to their small size, these microcompartments are not accessible by conventional microscopy. Here, we demonstrate that time-correlated single-photon counting (TCSPC) fluorescence lifetime-imaging microscopy (FLIM) classifies not only mitochondria, but different microcompartments inside mitochondria. Sensor proteins in the matrix had a different lifetime than probes at membrane proteins. Localization in the outer and inner mitochondrial membrane could be distinguished by significant differences in the lifetime. The method was sensitive enough to monitor shifts in protein location within mitochondrial microcompartments. Macromolecular crowding induced by changes in the protein content significantly affected the lifetime, while oxidizing conditions or physiological pH changes had only marginal effects. We suggest that FLIM is a versatile and completive method to monitor spatiotemporal events in mitochondria. The sensitivity in the time domain allows for gaining substantial information about sub-mitochondrial localization overcoming diffraction limitation. This article is part of a Special Issue entitled 'EBEC 2016: 19th European Bioenergetics Conference, Riva del Garda, Italy, July 2-6, 2016', edited by Prof. Paolo Bernardi. |
| ISSN | 00063002 |
| Journal | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer |
| Issue Number | 8 |
| Volume Number | 1857 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-08-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Electron Transport Complex III Genetics Mitochondria Metabolism Ultrastructure Optical Imaging A Kinase Anchor Proteins Bacterial Proteins Gene Expression Genes, Reporter Glycerol Pharmacology HeLa Cells Hydrogen Peroxide Hydrogen-Ion Concentration Luminescent Proteins Membrane Transport Proteins Drug Effects Instrumentation Receptors, Cell Surface Recombinant Fusion Proteins Research Support, Non-U.S. Gov't Biochemistry |
| Content Type | Text |
| Resource Type | Article |
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