NDLI: DNA-directed synthesis in vitro of T4 phage-specific enzymes.

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DNA-directed synthesis in vitro of T4 phage-specific enzymes.

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DNA-directed synthesis in vitro of T4 phage-specific enzymes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Natale, P. J. Buchanan, J. M.
Abstract	The synthesis of deoxynucleotide kinase (EC 2.7.4.2) in vitro by a preparation consisting of T4 bacteriophage DNA and a cell-free extract of Escherichia coli has been reported. A study of the role of monovalent cations in the synthesis of this enzyme as well as alpha-glucosyl transferase (EC 2.4.1.2) shows that potassium ions are required for maximal enzyme production. Examination of the RNA-directed system indicates that potassium ions are more effective than ammonium ion in the translation of messenger RNA for the formation of the biologically active proteins studied. From a comparison of the magnitude of the effect of ions on both the DNA- and RNA-directed systems, we conclude that potassium ions may also have a marked stimulatory effect on transcription of the deoxynucleotide kinase gene of T4 DNA. The time required for messenger initiation and completion and the size distribution of messenger RNA formed in vitro were also examined.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	9
Volume Number	69
Language	English
Publisher	National Academy of Sciences
Publisher Date	1972-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Coliphages Enzymology DNA, Viral Pharmacology Glucosyltransferases Biosynthesis Phosphotransferases Carbon Isotopes Centrifugation, Density Gradient Cytosine Nucleotides Deoxyribonucleotides Escherichia Coli Drug Effects Metabolism Kinetics Leucine Nucleic Acid Hybridization Nucleoside Diphosphate Sugars Potassium Quaternary Ammonium Compounds RNA, Messenger RNA, Viral Sodium Tritium Uracil Nucleotides Viral Proteins Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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