NDLI: Direction of transcription of the regulatory gene araC in Escherichia coli B-r.

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Direction of transcription of the regulatory gene araC in Escherichia coli B-r.

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Direction of transcription of the regulatory gene araC in Escherichia coli B-r.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wilcox, G. Boulter, J. Lee, N.
Abstract	The protein product of the regulatory gene araC can be synthesized in a cell-free, protein-synthesizing system programmed with a lambdaparaC(+)B DNA template. Hybrid, renatured phage DNA molecules prepared with DNA from phages lambdaparaC(+)B and lambdaparaC3B (araC3 is a nonsense mutation) were used to program the cell-free synthesis of the araC protein. The findings observed lead to the conclusion that the codogenic strand of the araC gene is on the light strand of the phage DNA. The araB gene is on the heavy strand, as determined by DNA.RNA hybridization. Thus, with regard to the standard E. coli map, araC is transcribed in a clockwise direction, whereas transcription of the araBAD operon has a counterclockwise orientation. The technique described should allow one to determine the direction of transcription of any gene that can be incorporated into the genome of a specialized transducing phage.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	9
Volume Number	71
Language	English
Publisher	National Academy of Sciences
Publisher Date	1974-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Arabinose Metabolism Escherichia Coli Genes, Regulator Transcription, Genetic Coliphages DNA Replication DNA, Viral Genetic Code Mutation Nucleic Acid Hybridization Templates, Genetic Transduction, Genetic Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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