NDLI: Antigen-induced conformational changes in antibodies and their Fab fragments studied by circular polarization of fluorescence.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1975 Volume: 72 Issue: 12

Year: 1975 Volume: 72 Issue: 11

Year: 1975 Volume: 72 Issue: 10

Year: 1975 Volume: 72 Issue: 9

Year: 1975 Volume: 72 Issue: 8

Year: 1975 Volume: 72 Issue: 7

Antigen mobility in membranes and complement-medical immune attack.

Attraction of the oriental fruit fly, Dacus dorsalis, to methyl eugenol and related olfactory stimulants.

Preparation and microbial decomposition of synthetic [14C]ligins.

Interaction of cholera toxin and membrane GM1 ganglioside of small intestine.

Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus.

Site-specific cleavage of single-stranded DNA by a Hemophilus restriction endonuclease.

Cholera toxin activation of adenylate cyclase in cancer cell membrane fragments.

Amino-acid sequence of activation cleavage site in plasminogen: homology with 'pro' part of prothrombin.

Molecular orbital studies of enzyme activity: I: Charge relay system and tetrahedral intermediate in acylation of serine proteinases.

Methylation of messenger RNA of Newcastle disease virus in vitro by a virion-associated enzyme.

Isolation of a cationic polypeptide from human serum that stimulates proliferation of 3T3 cells.

Minimal requirements for template recognition by bacteriophage Qbeta replicase: approach to general RNA-dependent RNA synthesis.

Apparent dependence of interactions between cytochrome b5 and cytochrome b5 reductase upon translational diffusion in dimyristoyl lecithin liposomes.

Protein-water interaction studied by solvent 1H, 2H, and 17O magnetic relaxation.

Hemoglobin synthesis in somatic cell hybrids: coexpression of mouse with human or chinese hamster globin genes in interspecific somatic cell hybrids of mouse erythroleukemia cells.

Chromatin architecture: investigation of a subunit of chromatin by dark field electron microscopy.

Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro.

Microfibril assembly by granules of chitin synthetase.

Establishment of tight junctions between epithelial cells.

Biosynthesis of delta-aminolevulinic acid from the intact carbon skeleton of glutamic acid in greening barley.

Myogenesis of avian striated muscle in vitro: role of collagen in myofiber formation.

Feedback dynamics analysis of secondary successional transients in ecosystems.

Interlocus variation of genetic distance and the neutral mutation theory.

Distribution of allelic frequencies in a finite population under stepwise production of neutral alleles.

Antigen-induced conformational changes in antibodies and their Fab fragments studied by circular polarization of fluorescence.

Lysis of leukemia cells by spleen cells of normal mice.

Interaction of lymphocytes with lipid bilayer membranes: a model for lymphocyte-mediated lysis of target cells.

Methylmalonicacidemia: biochemical heterogeneity in defects of 5'-deoxyadenosylcobalamin synthesis.

Fetal mouse hearts: a model for studying ischemia.

Ontogenesis of a biological clock for serotonin:acetyl coenzyme A N-acetyltransferase in pineal gland of rat.

Individual odor differences and their social functions in insects.

Slow growth rate of a deep-sea clam determined by 228Ra chronology.

In vivo and in vitro phosphorylation of DNA-dependent RNA polymerase of Escherichia coli by bacteriophage-T7-induced protein kinase.

Proton nuclear magnetic resonance of spin-labeled Escherichia coli tRNAf1MET.

Sulfonamide resistance mechanism in Escherichia coli: R plasmids can determine sulfonamide-resistant dihydropteroate synthases.

Release of (oligo) peptidyl-tRNA from ribosomes by erythromycin A.

Origin of hemopoietic stem cells in embryonic bursa of Fabricius and bone marrow studied through interspecific chimeras.

Conditional-lethal mutations that suppress genetic defects in morphogenesis by altering structural proteins.

Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits.

Inducible error-prone repair in Escherichia coli.

New chromosomal location for structural genes of ribosomal proteins.

A bacterial mutation blocking P2 phage late gene expression.

Somnolence, akinesia, and sensory activation of motivated behavior in the lateral hypothalamic syndrome.

Oxygen exchange in the gamma-phosphoryl group of protein-bound ATP during Mg2+-dependent adenosine triphosphatase activity of myosin.

Transformation by polyoma virus alters expression of a cell mutation affecting cycle traverse.

Coupled in vitro transcription and translation of vesicular stomatitis virus messenger RNA.

Characterization of purified DNA-relaxing enzyme from human tissue culture cells.

Homology and relationship between the genomes of papovaviruses, BK virus and simian virus 40.

RNAs of simian virus 40 in productively infected monkey cells: kinetics of formation and decay in enucleate cells.

Interaction of f1 histone with superhelical DNA.

Separation of 'estrogen-induced' protein from phosphoprotein phosphatase activity of immature rat uterus.

Association of mouse liver adenosine 3':5'-cyclic monophosphate (cyclic AMP) levels with histocompatibility-2 genotype.

Relationship of the first step in protein synthesis to ppGpp: formation of A(5')ppp(5')Gpp.

Solid tumor models for the assessment of different treatment modalities: I. Radiation-induced changes in growth rate characteristics of a solid tumor model.

Follicle-stimulating hormone stimulates estradiol-17beta synthesis in cultured Sertoli cells.

Appearance of smaller mannosyl-glycopeptides on the surface of a human cell transformed by simian virus 40.

New histones found in mature mammalian testes.

Initiation of cell proliferation in cultured mouse fibroblasts by prostaglandin F2alpha.

Extraction of Renilla-type luciferin from the calcium-activated photoproteins aequorin, mnemiopsin, and berovin.

Crosslinking and labeling of membrane proteins by transglutaminase-catalyzed reactions.

Functional arginyl residues as ATP binding sites of glutamine synthetase and carbamyl phosphate synthetase.

An octamer of histones in chromatin and free in solution.

Region of immunoglobulin light-chain mRNA transcribed into complementary DNA by RNA-dependent DNA polymerase of avian myeloblastosis virus.

Specific binding of tryptophan transfer RNA to avian myeloblastosis virus RNA-dependent DNA polymerase (reverse transcriptase).

Transformation of cultured human embryonic fibroblasts by oncornavirus-like particles released from a human carcinoma cell line.

Oncornavirus-like particles from cultured bone marrow cells preceding leukemia and malignant histiocytosis.

3'-Terminal nucletide sequence (n equals 361) of bacteriophage MS2 RNA.

Metabolic pathways of 7,12-dimethylbenz[a]anthracene in hepatic microsomes.

Absence of histone F1 in a mitotically dividing, genetically inactive nucleus.

On the question of the integration of exogenous bacterial DNA into plant DNA.

Evolutionary and structural influences on light chain constant (CL) region of human and mouse immunoglobulins.

Year: 1975 Volume: 72 Issue: 6

Year: 1975 Volume: 72 Issue: 5

Year: 1975 Volume: 72 Issue: 4

Year: 1975 Volume: 72 Issue: 3

Year: 1975 Volume: 72 Issue: 2

Year: 1975 Volume: 72 Issue: 1

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Antigen-induced conformational changes in antibodies and their Fab fragments studied by circular polarization of fluorescence.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Steinberg, I. Z. Schlessinger, J. Givol, D. Pecht, I. Hochman, J.
Abstract	Conformational changes induced in antibody molecules and in their Fab fragments by binding of antigen were investigated by the circular polarization of the fluorescence emitted by the tryptophan residues. This property of the fluorescence is related to the asymmetry, and thus to the conformation and environment, of the emitting chromophore. Changes in the circular polarization of the fluorescence of the antibody were observed upon binding of RNase to anti-RNase, of poly(DL-alanyl)-poly(L-lysine) to antipoly(D-alanine), and of the 'loop' of lysozyme, a monovalent antigenic determinant, to anti'loop.' The spectral changes were observed at different antigen-antibody ratios, including high antigen excess, indicating that they are due to antigen binding and not to aggregation. The circular polarization of fluorescence also detects changes in conformation of the different Fab fragments upon binding of the corresponding antigens. These changes in conformation were, however, markedly different from those observed for the whole antibody molecules, and indicated an interaction between the Fc and Fab fragments in the antibody molecule, and probably a change in the conformation of Fc upon binding of antigen to the antibody. In contrast, the small hapten, phosphorylcholine, did not induce a change in the circular polarization of the fluorescence of its antibody or corresponding Fab fragments. Reduction of the interchain disulfide bonds of the antibodies abolished the antigen-induced spectral changes due to the presence of the Fc portion in the molecule, but not the changes observed in Fab, suggesting that the disulfide bonds at the hinge region of the antibody are required for the transmission of the conformational change from the Fab to the Fc.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	7
Volume Number	72
Language	English
Publisher	National Academy of Sciences
Publisher Date	1975-12-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Antibodies Antigens Immunoglobulin Fab Fragments Animals Antigen-Antibody Reactions Binding Sites Binding Sites, Antibody Chromatography, Affinity Disulfides Immunoglobulin Fc Fragments Muramidase Immunology Protein Binding Protein Conformation Rabbits Ribonucleases Spectrometry, Fluorescence Spectrum Analysis Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in