NDLI: Use of gene 32 protein staining of single-strand polynucleotides for gene mapping by electron microscopy: application to the phi80d3ilvsu+7 system.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1975 Volume: 72 Issue: 12

Year: 1975 Volume: 72 Issue: 11

Raman resonance of electron donor/acceptor complex.

The extraordinary dielectric properties of biological materials and the action of enzymes.

Resonance Raman spectroscopic studies of the interactions between trypsin and a competitive inhibitor.

Allosteric mechanism for codon-dependent tRNA selection on ribosomes.

Nucleotide sequence of microvariant RNA: another small replicating molecule.

Temporal changes in tectal cell surface specificity induced by nerve growth factor.

Action of nicking-closing enzyme on supercoiled and nonsupercoiled closed circular DNA: formation of a Boltzmann distribution of topological isomers.

Functional level of rat liver tryptophan 2,3-dixoygenase messenger RNA during superinduction of enzyme with actinomycin D.

Characterization of DNA condensates induced by poly(ethylene oxide) and polylysine.

Kinetics of binding of carbon monoxide to lumbricus erythrocruorin: a possible model.

Genome-sized RNA from reovirus particles.

Pituitary growth hormones: further evidence for evolutionary conservatism based on immunochemical studies.

General model for nutritional responses of higher organisms.

Regulation of synthesis of hepatic fatty acid synthetase: polysomal translation in a cell-free system.

Acetylcholine-receptor-mediated ion flux in electroplax membrane preparations.

Brain receptors for antipsychotic drugs and dopamine: direct binding assays.

Role of carnitine in hepatic ketogenesis.

Initiation of in vitro mRNA synthesis from the wild-type lac promoter.

Influence of insertions on packaging of host sequences covalently linked to bacteriophage Mu DNA.

Structure of transcriptionally active chromatin.

Fusion of dipalmitoylphosphatidylcholine vesicle membranes induced by concanavalin A.

Resonance Raman effect in mu-oxo-bis[iron(III) tetraphenylporphyrin].

Chemical induction of sperm abnormalities in mice.

Local anesthetics affect transmembrane cytoskeletal control of mobility and distribution of cell surface receptors.

Adenovirus-2 mRNA is transcribed as part of a high-molecular-weight precursor RNA.

Selective effects of inhibitors of protein synthesis on metabolism of nuclear anc cytoplasmic proteins: evidence for coordinate synthesis of non-histone chromosomal proteins.

Ectopic neurons and aberrant connections during neural development.

Turning on of activities in unfertilized sea urchin eggs: correlation with changes of the surface.

Relationship between release of surface proteins and metabolic activation of sea urchin eggs at fertilization.

Serum rapidly stimulates ouabain-sensitive 86-RB+ influx in quiescent 3T3 cells.

Specific cleavage analysis of mammalian mitochondrial DNA.

Use of gene 32 protein staining of single-strand polynucleotides for gene mapping by electron microscopy: application to the phi80d3ilvsu+7 system.

Analysis of changes in collagen biosynthesis that occur when chick chondrocytes are grown in 5-bromo-2'-deoxyuridine.

Colchicine permeation is required for inhibition of concanavalin A capping in Chinese hamster ovary cells.

Allozymic and chromosomal similarity in two Drosophila species.

Gene dosage: evidence for assignment of erythrocyte acid phosphatase locus to chromosome 2.

General kin selection models for genetic evolution of sib altruism in diploid and haplodiploid species.

Effect of temporal fluctuation of selection coefficient on gene frequency in a population.

Genetic analysis in the dinoflagellate (Crypthecodinium (Gyrodinium) cohnii: evidence for unusual meiosis.

Guinea pig transfer factor-like activity detected in vitro.

Genome of hepatitis B virus: restriction enzyme cleavage and structure of DNA extracted from Dane particles.

Measurement of cholesterol synthesis in man by isotope kinetics of squalene.

An inherited 'neoplasm' in fungus.

Dopamine receptor binding in the corpus striatum of mammalian brain.

Studies on memory: inhibitors of protein synthesis also inhibit catecholamine synthesis.

Mechanism of dissociation of ribosomes of Escherichia coli by initiation factor IF-3.

Identification of a soluble protein that stimulates peptide bond synthesis.

Enzymatic induction of DNA double-strand breaks in gamma-irradiated Escherichia coli K-12.

Quaternary constraint in hybrid of aspartate transcarbamylase containing wild-type and mutant catalytic subunits.

Structural properties of pyruvate carboxylases from chicken liver and other sources.

Identification of a region in 23S rRNA located at the peptidyl transferase center.

Biochemical construction and selection of hybrid plasmids containing specific segments of the Escherichia coli genome.

Guanosine 5'-diphosphate 3'-diphosphate (ppGpp): positive effector for histidine operon transcription and general signal for amino-acid deficiency.

Structure of yeast phenylalanine transfer RNA at 2.5 A resolution.

Two stages in lymphocyte mediator production by differential susceptibility to blockade using niridazole.

Association of salmonella mutants with germfree rats: site specific model to detect carcinogens as mutagens.

Role of methionine in bacterial chemotaxis: requirement for tumbling and involvement in information processing.

Induction of error-prone repair as a consequence of DNA ligase deficiency in Escherichia coli.

Influence of an aggregated multienzyme system on transient time: kinetic evidence for compartmentation by an aromatic-amino-acid synthesizing complex of Neurospora crassa.

Viability of Escherichia coli treated with colicin K.

Effects of estrogen on gene expression in chick oviduct: nuclear receptor levels and initiation of transcription.

Anatomy of herpes simplex virus DNA: evidence for four populations of molecules that differ in the relative orientations of their long and short components.

Conformational fluctuations of DNA helix.

Translation of RNA that contains polyadenylate from yeast mitochondria in an Escherichia coli ribosomal system.

Androgen- and estrogen-binding macromolecules in developing mouse brain: biochemical and genetic evidence.

Requirement for cellular protein synthesis in reversal of ethidium-bormide-induced inhibition of cell transformation by murine sarcoma virus.

Effect of extradiol-17beta on the synthesis of specific uterine nonhistone chromosomal proteins.

Thermolabile T (tumor) antigen from cells transformed by a temperature-sensitive mutant of simian virus 40.

In vitro synthesis of a constitutive enzyme of Escherichia coli, 6-phosphogluconate dehydrogenase.

Tumorigenicity of virus-transformed cells in nude mice is correlated specifically with anchorage independent growth in vitro.

Deposition of histones onto replicating chromosomes.

Rous sarcoma virus activates embryonic globin genes in chicken fibroblasts.

Cytochemical study of secretory process in transplantable insulinoma of syrian golden hamster.

Relationship in nucleic acid sequences between mouse mammary tumor virus variants.

Separation of sarcoma virus-specific and leukemia virus-specific genetic sequences of Moloney sarcoma virus.

Filamin, a new high-molecular-weight protein found in smooth muscle and non-muscle cells.

Immunochemical characterization of murine H-2 controlled Ss (serum substance) protein through identification of its human homologue as the fourth component of complement.

Induction of an immune response through interaction of helper cells with an immune complex bound to the surface of B cells.

Structural features of double-stranded polyribonucleotides required for immunological specificity and interferon induction.

Activation of T and B lymphocytes in vitro: presence of beta2-microblobulin determinants on allogeneic effect factor.

Stimulation and inhibition of anti-hapten responses in guinea pigs immunized with hybrid liposomes.

Multiple active sites on human interferons.

Oncornavirus-like particles released from arginine-deprived human lymphoblastoid cell lines.

Carcinoembryonic antigen: evidence for multiple antigenic determinants and isoantigens.

Identification of NADPH-thioredoxin reductase system in Euglena gracillis.

Purification of folate binding factor in normal umbilical cord serum.

Intramolecular arsanilazotyrosine-248-Zn complex of carboxypeptidase A: a monitor of multiple conformational states in solution.

Presence of norepinephrine and related enzymes in isolated brain microvessels.

Evidence of homologous relationship between thermolysin and neutral protease A of Bacillus subtilis.

Contraction of isolated smooth muscle cells by inophore A23187.

Guanosine metabolism and regulation of fruiting body construction in dictyostelium discoideum.

Structure of tight junctions in epithelia with different permeability.

Chromosome puff activity and protein synthesis in larval salivary glands of Drosophila melanogaster.

Primary structure of a deleted human lambda type immunoglobulin light chain containing carbohydrate: protein Sm lambda.

Evolution of human longevity and the genetic complexity governing aging rate.

Aging and its relation to cell growth and differentiation in Drosophila imaginal discs: developmental response to growth restricting conditions.

Year: 1975 Volume: 72 Issue: 10

Year: 1975 Volume: 72 Issue: 9

Year: 1975 Volume: 72 Issue: 8

Year: 1975 Volume: 72 Issue: 7

Year: 1975 Volume: 72 Issue: 6

Year: 1975 Volume: 72 Issue: 5

Year: 1975 Volume: 72 Issue: 4

Year: 1975 Volume: 72 Issue: 3

Year: 1975 Volume: 72 Issue: 2

Year: 1975 Volume: 72 Issue: 1

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Use of gene 32 protein staining of single-strand polynucleotides for gene mapping by electron microscopy: application to the phi80d3ilvsu+7 system.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Davidson, N. Wu, M.
Abstract	A method for visualizing RNA-DNA duplex regions along a single strand of DNA in the electron microscope is described. A preparation of RNA molecules is hybridized to a long DNA strand containing the coding sequences (genes) for some of the RNAs. T4 gene 32 protein, which binds selectively and cooperatively only to the single-strand regions, is added, followed by glutaraldehyde. The resulting nucleic acid-gene 32 complex is adsorbed to the surface of an electron microscope grid in the presence of ethidium bromide. The single-strand regions are relatively thick (8.5 nm) compared to the duplex (RNA-DNA hybrid) regions (3.5 nm), so that the two kinds of regions are readily recognized by electron microscopy. In favorable cases, tRNA-DNA hybrids of length about 80 nucleotide pairs can be recognized (although with difficulty). The positions of a number of interesting genetic sequences on the DNA of the transducing phage phi80d3ilvsu+7 have been mapped. The r strand contains 16S, 23S, and 5S rRNA coding sequences in that order. The spacer between 16S and 23S genes has a length of 500 nucleotides and contains the coding sequence for a tRNA2Glu gene in agreement with previous biochemical observations. The spacer between the 23S and 5S genes has a length of 180 nucleotides. The su+7 tRNATrp coding sequence has been mapped on the l strand at a position just to the left of the ilv genes. Secondary structure loops due to short inverted repeat sequences flanking the 16S, 23S, tRNATrp, and F sequences in the DNA have been observed.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	11
Volume Number	72
Language	English
Publisher	National Academy of Sciences
Publisher Date	1975-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Chromosome Mapping DNA, Bacterial DNA, Single-Stranded Genes Coliphages DNA, Viral Ethidium Microscopy, Electron Nucleic Acid Hybridization RNA, Bacterial Metabolism RNA, Ribosomal RNA, Transfer Transduction, Genetic Viral Proteins Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in