NDLI: Specific release of the thioesterase component of the fatty acid synthetase multienzyme complex by limited trypsinization.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1976 Volume: 73 Issue: 12

Year: 1976 Volume: 73 Issue: 11

Year: 1976 Volume: 73 Issue: 10

Year: 1976 Volume: 73 Issue: 9

Year: 1976 Volume: 73 Issue: 8

Year: 1976 Volume: 73 Issue: 7

Year: 1976 Volume: 73 Issue: 6

Year: 1976 Volume: 73 Issue: 5

Year: 1976 Volume: 73 Issue: 4

Biological activity of catecholamines covalently linked to synthetic polymers: proof of immobilized drug theory.

Distribution of 5-bromodeoxyuridine and thymidine in the DNA of developing chick cartilage.

Intracellular forms of adenovirus DNA: integrated form of adenovirus DNA appears early in productive infection.

New protein in human blood plasma, rich in proline, with lipid-binding properties.

A thermodynamic model of regulation: modulation of redox equilibria in camphor monoxygenase.

Ribonuclease H of calf thymus: substrate specificity, activation, inhibition.

Reconstitution of Qbeta RNA replicase from a covalently bonded elongation factor Tu-Ts complex.

Intracellular localization of mouse DNA polymerase-alpha.

Spectral differences between the alpha and beta heme groups within human deoxyhemoglobin.

Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands.

Hydrolytic stability of helical RNA: a selective advantage for the natural 3',5'-bond.

Direct experimental evidence for kinetic proofreading in amino acylation of tRNAIle.

An altered apurinic DNA endonuclease activity in group A and group D xeroderma pigmentosum fibroblasts.

mRNA-dependent synthesis of authentic precursor to human placental lactogen: conversion to its mature hormone form in ascites cell-free extracts.

Specific release of the thioesterase component of the fatty acid synthetase multienzyme complex by limited trypsinization.

Electron probe x-ray analysis of single ferritin molecules.

Somatostatin conformation: evidence for a stable intramolecular structure from circular dichroism, diffusion, and sedimentation equilibrium.

Theoretical studies on pro-leu-gly-nh2 conformation.

Transepithelial transport in cell culture.

Reversible in vitro polymerization of tubulin from a cultured cell line (rat glial cell clone C6).

Clathrin: a unique protein associated with intracellular transfer of membrane by coated vesicles.

Germ line integration and Mendelian transmission of the exogenous Moloney leukemia virus.

Influence of diet on survival of mice.

Interferon treatment of mice: enhanced expression of histocompatibility antigens on lymphoid cells.

Measles virus-specific IgD antibodies in patients with subacute sclerosing panencephalitis.

Genetic polymorphism of IgD-like cell surface immunoglobulin in the mouse.

Fluorometric assay of vasopressin and oxytocin: a general approach to the assay of peptides in tissues.

Cancer-associated isoenzyme of serum galactosyltransferase.

Porphyria variegata and porphyria cutanea tarda in siblings: chemical and genetic aspects (addendum).

Eel esophagus as an osmoregulatory organ.

Pharmacologic characterization of the Na+ ionophores in L6 myotubes.

Rates, patterns, and effectiveness of evolution in multi-level situations.

An experimental analysis of competitive indeterminacy in Tribolium.

Simple synthesis of a 4a-hydroperoxy adduct of a 1,5-dihydroflavine: preliminary studies of a model for bacterial luciferase.

Distribution of Ia-like molecules on the surface of normal and leukemic human cells.

Antigen stimulation of prostaglandin synthesis and control of immune responses.

Molecular abnormality of human alpha1-antitrypsin variant (Pi-ZZ) associated with plasma activity deficiency.

Müller cell function during spreading depression in frog retina.

Hypertrophy and hyperplasia of somatostatin-containing D-cells in diabetes.

Functional incorporation of ganglioside into intact cells: induction of choleragen responsiveness.

Synthetic hexapeptide substrates and inhibitors of 3':5'-cyclic AMP-dependent protein kinase.

Construction and propagation of a defective simian virus 40 genome bearing an operator from bacteriophage lambda.

Distribution of envelope-specific and sarcoma-specific nucleotide sequences from different parents in the RNAs of avian tumor virus recombinants.

Deficiency in histone acetylation in nontransforming host range mutants of polyoma virus.

Two forms of simian-virus-40-specific T-antigen in abortive and lytic infection.

Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases.

Control of protein synthesis in reticulocyte lysates: effects of 3':5'-cyclic AMP, ATP, and GTP on inhibitions induced by hemedeficiency, double-stranded RNA, and a reticulocyte translationa inhibitor.

Transcription-translation and translation-messenger RNA decay coupling: separate mechanisms for different messengers.

Dephosphorylation of nonhistone proteins specifically alters the pattern of gene transcription in reconstituted chromatin.

Cell surface protein partially restores morphology, adhesiveness, and contact inhibition of movement to transformed fibroblasts.

Erythroid cell differentiation: murine erythroleukemia cell variant with unique pattern of induction by polar compounds.

Activation of 3':5'-cyclic AMP-dependent protein kinase and induction of ornithine decarboxylase as early events in induction of mixed-function oxygenases.

A differentiation stimulating factor induces cell sensitivity to 3':5'-cyclic AMP pulses in dictyostelium discoideum.

Proliferation of Rous sarcoma virus-infected, but not of normal, chicken fibroblasts in oxygen-enriched environment: preliminary report.

Cotransfer of two linked human genes into cultured mouse cells.

Human papilloma virus DNA: physical mapping and genetic heterogeneity.

Activation and nuclear translocation of protein kinase during transsynaptic induction of tyrosine 3-monooxygenase.

A 15-hydroxyprostaglandin dehydrogenase specific for prostaglandin A in rabbit kidney.

Specific positions involved in enzyme catalyzed covalent binding of benzo[a]pyrene to poly(G).

Simple model for hormone-activated adenylate cyclase systems.

Heat mutagenesis in bacteriophage T4: the transition pathway.

beta-adrenergic receptors in rat liver: effects of adrenalectomy.

Thermodynamic studies of polymerization of deoxygenated sickle cell hemoglobin.

Bovine leukemia virus: an exogenous RNA oncogenic virus.

Synthesis of murine leukemia virus proteins associated with virions assembled in actinomycin D-treated cells: evidence for persistence of viral messenger RNA.

Immunologic and fine structure evidence of avidly bound host serum proteins in the surface coat of a bloodstream trypanosome.

Antisera against electrophoretically purified tubulin stimulate colchicine-binding activity.

Evidence for circularization of the avian oncornavirus RNA genome during proviral DNA synthesis from studies of reverse transcription in vitro.

Pheasant virus: new class of ribodeoxyvirus.

Molecular structure of yeast RNA polymerase III: demonstration of the tripartite transcriptive system in lower eukaryotes.

Transcription in yeast: alpha-amanitin sensitivity and other properties which distinguish between RNA polymerases I and III.

Involvement of escherichia coli dnaZ gene product in DNA elongation in vitro.

Variant forms of mitochondrial translation products in yeast: evidence for location of determinants on mitochondrial DNA.

Double-stranded RNA in chromatin transcripts formed by exogenous RNA polymerase.

Asymmetric orientation of phage M13 coat protein in Escherichia coli cytoplasmic membranes and in synthetic lipid vesicles.

Acquisition of substrate-specific parameters during the catalytic reaction of penicillinase.

Dimethylsulfoxide-induced differentiation and hemoglobin synthesis in tissue cultures of rat erythroleukemia cells transformed by 7,12-dimethylbenz(a)anthracene.

Immunoglobulin allotypes of rabbit kappa chains: polymorphism of a control mechanism regulating closely linked duplicated genes?

Year: 1976 Volume: 73 Issue: 3

Year: 1976 Volume: 73 Issue: 2

Year: 1976 Volume: 73 Issue: 1

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Specific release of the thioesterase component of the fatty acid synthetase multienzyme complex by limited trypsinization.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Dileepan, K. N. Libertini, L. Smith, S. Agradi, E.
Abstract	Limited trypsinization of the fatty acid synthetase multienzyme complex from rat mammary gland results in the release of a protein, molecular weight 32,000, with thioesterase activity. The other components of the multienzyme complex--the acyl carrier protein, acetyl and malonyl transferases, condensing enzyme, keto reductase, dehydrase and enoyl reductase--are not affected and remain associated with the complex. The thioesterase can be isolated by ammonium sulfate precipitation and gel filtration. Extensive trypsinization of fatty acid synthetase multienzyme complex results in a loss of thioesterase activity, probably due to cleavage of the thioesterase component into inactive peptides. However, the molecular weight and specific activity of the thioesterase isolated after limited trypsinization is relatively unaffected by the severity of the conditions of proteolysis. Both the thioesterase and the residual trypsinized complex react with antibodies produced against the native multienzyme. The results demonstrate that mild trypsinization can be used to release the thioesterase component of the multienzyme with little perturbation of either the thioesterase or the other components of the complex.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	4
Volume Number	73
Language	English
Publisher	National Academy of Sciences
Publisher Date	1976-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Fatty Acid Synthases Thiolester Hydrolases Isolation & Purification Animals Cross Reactions Immunology Mammary Glands, Animal Enzymology Molecular Weight Metabolism Trypsin Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in