NDLI: Binding of triton X-100 to diphtheria toxin, crossreacting material 45, and their fragments.

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Binding of triton X-100 to diphtheria toxin, crossreacting material 45, and their fragments.

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Binding of triton X-100 to diphtheria toxin, crossreacting material 45, and their fragments.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Silverman, M. S. Boquet, P. Pappenheimer, A. M. Vernon, W. B.
Abstract	Binding of the nonionic detergent [3H]Triton X-100 by diphtheria toxin, by the nontoxic serologically related protein crossreacting material (CRM) 45, and by their respective A and B fragments has been studied. If first denatured in 0.1% sodium dodecyl sulfate, all of the proteins with the exception of fragment A bind increasing amounts of Triton X-100, reaching a maximum of more than 40 mol bound per mol of protein when the detergent concentration exceeds its critical micelle concentration. No measurable amount of Triton X-100 is bound by native toxin or its A fragment of any concentration of the detergent. Undenatured CRM45 or its B45 fragment, on the other hand, readily became inserted into Triton X-100 micelles when the detergent reaches its critical micelle concentration. The results show that the toxin molecule contains a hydrophobic domain located on the portion of the B fragment that is linked to A. This region is masked in native toxin. Based on these findings, a model is proposed to describe how fragment B facilitates the transport of the enzymically active hydrophilic fragment A across the plasma membrane to reach the cytoplasm.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	12
Volume Number	73
Language	English
Publisher	National Academy of Sciences
Publisher Date	1976-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Diphtheria Toxin Metabolism Polyethylene Glycols Quaternary Ammonium Compounds Binding Sites Biological Transport Epitopes Models, Biological Peptides Solubility Structure-Activity Relationship Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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