NDLI: A rapid procedure for selective enrichment of photosynthetic electron transport mutants.

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A rapid procedure for selective enrichment of photosynthetic electron transport mutants.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Matlin, K. S. Schmidt, G. W. Chua, N. H.
Abstract	Metronidazole (2-methyl-5-nitroimidazole-l-ethanol) is shown to be effective for the selective enrichment of mutants of Chlamydomonas reinhardtii that posses impaired photosynthetic electron transport. More than 99.9% of wild-type cells are killed when incubated in the presence of 6-10 mM metronidazole for 24 hr under illumination of 7500 lux. Survival of wild-type cells in darkness and of mutants that are blocked at different steps in photosynthetic electron transport is nearly 100% when incubated in the presence of the drug under identical conditions. The toxicity of metronidazole is demonstrated to depend upon its reduction by photosynthetic electron transport. Light-dependent oxygen uptake mediated by metronidazole is shown to require active photosystem l in vitro and in vivo. Ferredoxin is necessary for metronidazole reduction by thylakoid membrane fractions enriched in photosystem l activity. We propose that the toxicity of metronidazole is due to the formation of lethal derivatives of the drug or to the accumulation of hydrogen peroxide, which could occur upon autooxidation of metronidazole reduced by one electron. The results indicate that mutants of C. reinhardtii, and probably other photosynthetic organisms, with any lesion in photosynthetic electron transport from the oxidizing side of photosystem ll to ferredoxin can be isolated by metronidazole treatment of mutagenized cultures.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	2
Volume Number	74
Language	English
Publisher	National Academy of Sciences
Publisher Date	1977-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Chlamydomonas Metabolism Photosynthesis Cell Membrane Cell Survival Drug Effects Digitonin Diuron Pharmacology Electron Transport Kinetics Metronidazole Mutation Oxygen Photophosphorylation Species Specificity Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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