NDLI: Affinity of intact Escherichia coli for hydrophobic membrane probes is a function of the physiological state of the cells.

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Affinity of intact Escherichia coli for hydrophobic membrane probes is a function of the physiological state of the cells.

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Affinity of intact Escherichia coli for hydrophobic membrane probes is a function of the physiological state of the cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Nieva-gomez, D. Gennis, R. B.
Abstract	The fluorescence parameters of several common membrane probes in the presence of whole E. coli have been examined. The probes included electrically neutral lipophilic molecules N-phenyl-1-naphthylamine, pyrene, and 1,6-diphenyl-1,3,5-hexatriene as well as the negatively charged molecule 8-anilino-1-naphthalene sulfonate. It is demonstrated in each case that certain fluorescence parameters are a function of the state of energization of the cells. All the probes appear to monitor structural changes in the E. coli envelope which accompany the energization and de-energization of the cells. tthe phenomenon is completely reversible as demonstrated by re-energizing anoxic cells by the addition of oxygen, or starved cells by the addition of substrate. All the results are qualitatively consistent with an increased binding of probe by de-energized cells and a subsequent expulsion of probe when the cells are re-energized. A pyrene substituted with a photosensitive group, 1-azidopyrene, has been synthesized. Photolysis in the presence of a suspension of energized E. coli reveals a relatively small amount of probe irreversibly bound to the cells. However, in the presence of cells that have been de-energized the amount of irreversibly bound probe is dramatically increased. This molecule should be useful for localizing the regions of the bacterial envelope that are involved in the structural changes being monitored in these experiments.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	5
Volume Number	74
Language	English
Publisher	National Academy of Sciences
Publisher Date	1977-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Energy Metabolism Escherichia Coli Metabolism Fluorescent Dyes Membrane Lipids Colicins Pharmacology Drug Effects Anoxia Photochemistry Pyrenes Solubility Spectrometry, Fluorescence Starvation Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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