NDLI: Biological activity of cloned Moloney sarcoma virus DNA: Terminally redundant sequences may enhance transformation efficiency.

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Biological activity of cloned Moloney sarcoma virus DNA: Terminally redundant sequences may enhance transformation efficiency.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Oskarsson, M. K. Vande Woude, G. F. Fischinger, P. J. Blair, D. G. Mcclements, W. L.
Abstract	We have measured the ability of cloned restriction fragments containing the whole and partial genomes of two strains of Moloney murine sarcoma virus to induce cell transformation in DNA transfection assays. The cloned intact ml and HTl murine sarcoma virus proviruses transform with an efficiency of approximately 40,000-50,000 focus-forming units/pmol of proviral DNA, and the majority of these transformed cells contain a rescuable viral genome. A cloned 2.1-kilobase-pair internal fragment of the murine sarcoma virus containing 1.2 kilobase pairs of sarcoma virus-specific sequences (src) and approximately 900 base pairs of leukemia virus-derived sequences adjacent to the 5' end of src transforms with approximately 1/10,000th the efficiency of the intact genome. When leukemia virus-deprived sequences containing a single copy of the 600-base-pair direct terminal repeated sequences are present at either the 5' or 3' end of this src-containing fragment, the transforming activity is stimulated 1000-fold. Cotransfection with a mixture of cloned fragments, one containing the internal 2.1-kilobase-pair src fragment and the other containing a single copy of the terminally redundant sequence, results in a 300-fold increase in transformation efficiency.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	6
Volume Number	77
Language	English
Publisher	National Academy of Sciences
Publisher Date	1980-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Transformation, Viral DNA, Viral Metabolism Sarcoma Viruses, Murine Genetics Animals Bacteriophage Lambda Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant Escherichia Coli Genetic Vectors Helper Viruses Mice Mink Moloney Murine Leukemia Virus Plasmids Transfection Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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