NDLI: Cotransfer of circular and linear prokaryotic and eukaryotic DNA sequences into mouse cells.

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Isolation and structure of pro-somatostatin: a putative somatostatin precursor from pig hypothalamus.

gamma-Glutamylamine cyclotransferase: specificity toward epsilon-(L-gamma-glutamyl)-L-lysine and related compounds.

Agonist versus antagonist binding to alpha-adrenergic receptors.

Activation of brain tryptophan hydroxylase by ATP-MG2+: dependence on calmodulin.

Somatostatin: an endogenous peptide in the toad urinary bladder inhibits vasopressin-stimulated water flow.

Urotensin II: a somatostatin-like peptide in the caudal neurosecretory system of fishes.

Abnormal behavior of interferon-induced enzymatic activities in an interferon-resistant cell line.

Identification of folate binding protein of mitochondria as dimethylglycine dehydrogenase.

Intermediate filaments of baby hamster kidney (BHK-21) cells and bovine epidermal keratinocytes have similar ultrastructures and subunit domain structures.

Reactivity of reverse transcriptase toward (s4U,U)n copolymers and spin-labeled nucleic acid lattices.

Chemical probes for higher-order structure in RNA.

Fidelity of structure representation in electron micrographs of negatively stained protein molecules.

Synthesis of new proteins associated with the induction of interferon in human fibroblast cells.

Liver tumors distinguished by immunofluorescence microscopy with antibodies to proteins of intermediate-sized filaments.

Separation of random fragments of DNA according to properties of their sequences.

Mapping of anion binding sites on cytochrome c by differential chemical modification of lysine residues.

Photoaffinity label for the beta-adrenergic receptor: synthesis and effects on isoproterenol-stimulated adenylate cyclase.

Genomic changes associated with antigenic variation of visna virus durig persistent infection.

Direct inhibition of testicular function by gonadotropin-releasing hormone: mediation by specific gonadotropin-releasing hormone receptors in interstitial cells.

Possible role of distinct morphine and enkephalin receptors in mediating actins of benzomorphan drugs (putative kappa and sigma agonists).

Integration in vivo into simian virus 40 DNA of a sequence that resembles a certain family of genomic interspersed repeated sequences.

Ouabain-binding-site photoaffinity probes that label both subunits of Na+,K+-ATPase.

Genomic organization of rat prolactin and growth hormone genes.

Apurinic/apyrimidinic endonucleases in repair of pyrimidine dimers and other lesions in DNA.

Amino acid sequence of the T4 DNA helix-destabilizing protein.

Solubilization of active opiate receptors.

Adrenocorticotropic hormone increases specific proteins of the mitochondrial fraction that are translated inside or outside this organelle in cultured adrenal tumor cells.

Polycistronic vesicular stomatitis virus RNA transcripts.

Mapping of transforming region of the Harvey murine sarcoma virus genome by using insertion-deletion mutants constructed in vitro.

Surface charge changes in purple membranes and the photoreaction cycle of bacteriorhodopsin.

Similarities and dissimilarities between calmodulin and a Chlamydomonas flagellar protein.

Desensitization of enucleated cells to hormones and role of cytoskeleton in control of normal hormonal response.

Genetic recombination of bacterial plasmid DNA: electron microscopic analysis of in vitro intramolecular recombination.

Cotransfer of circular and linear prokaryotic and eukaryotic DNA sequences into mouse cells.

Indiscriminate recombination in simian virus 40-infected monkey cells.

Germ-line reinsertions of AKR murine leukemia virus genomes in Akv-1 congenic mice.

DNA-transformed murine teratocarcinoma cells: regulation of expression of simian virus 40 tumor antigen in stem versus differentiated cells.

Control of gene expression by a mobile recombinational switch.

Structural organization of human genomic DNA encoding the pro-opiomelanocortin peptide.

Induction of lymphoma in athymic mice: a model for study of the human disease.

Appearance of cytoskeletal components on the surface of leukemia cells and of lymphocytes transformed by mitogens and Epstein-Barr virus.

A transformation-defective mutant of Abelson murine leukemia virus lacks protein kinase activity.

An attempt to distinguish between the actions of neuromuscular blocking drugs on the acetylcholine receptor and on its associated ionic channel

Further evidence for peptidergic transmission in sympathetic ganglia.

Serotonin-induced hyperpolarization of an indentified Aplysia neuron is mediated by cyclic AMP.

Biochemical studies of olfaction: isolation, characterization, and odorant binding activity of cilia from rainbow trout olfactory rosettes.

Quercetin inhibits Ca2+ uptake but not Ca2+ release by sarcoplasmic reticulum in skinned muscle fibers.

Isolation of a heparan sulfate-containing proteoglycan from basement membrane.

Eukaryotic DNA segments capable of autonomous replication in yeast.

Selection of viability at loci controlling protein polymorphisms in Drosophila melanogaster is very weak at most.

Complete amino acid sequence of a mouse immunoglobulin alpha chain (MOPC 511).

Partial sequence of human complement component factor B: novel type of serine protease.

Efficient genetically controlled formation of antibody to a synthetic antigen [poly(LTyr, LGlu)-poly(DLAla)- -poly(LLys)] covalently bound to a synthetic adjuvant (N-acetylmuramyl-L-alanyl-D-isoglutamine).

Active site amino acid sequence of human factor D.

Somatic rearrangements forming active immunoglobulin mu genes in B and T lymphoid cell lines.

Blockade of recruitment of ovarian follicles by suppression of the secondary surge of follicle-stimulating hormone with porcine follicular field.

Uptake of free choline by isolated perfused rat liver.

Observation of an exothermic process associated with the in vitro polymerization of brain tubulin.

Targeting of lipid vesicles: specificity of carbohydrate receptor analogues for leukocytes in mice.

Characterization of rat calcitonin mRNA.

Collagen-tailed and hydrophobic components of acetylcholinesterase in Torpedo marmorata electric organ.

Coordinate regulation of two estrogen-dependent genes in avian liver.

Direct spectroscopic studies of cation translocation by Torpedo acetylcholine receptor on a time scale of physiological relevance.

Molecular cloning of developmentally regulated, low-abundance mRNA sequences from embryonic muscle.

Molecular dissection of Rauscher virus gp70 by using monoclonal antibodies: localization of acquired sequences of related envelope gene recombinants.

Antibody-directed cytotoxic agents: use of monoclonal antibody to direct the action of toxin A chains to colorectal carcinoma cells.

Excretion of hepatitis B surface antigen particles from mouse cells transformed with cloned viral DNA.

Fungal oxidation of (+/-)-9,10-dihydroxy-9,10-dihydrobenzo[a]pyrene: formation of diastereomeric benzo[a]pyrene 9,10-diol 7,8-epoxides.

Hormonal and immunological aspects of the phylogeny of sex steroid binding plasma protein.

beta-Endorphin: stability, clearance behavior, and entry into the central nervous system after intravenous injection of the tritiated peptide in rats and rabbits.

Conformation of poly(dG-dC) . poly(dG-dC) modified by the carcinogens N-acetoxy-N-acetyl-2-aminofluorene and N-hydroxy-N-2-aminofluorene.

ATP-driven steady-state exchange of monomeric and filamentous actin from Dictyostelium discoideum.

Elongation mechanism and substrate specificity of 2',5'-oligoadenylate synthetase.

Partial purification and characterization of a macromolecule which enhances fluoride activation of adenylate cyclase.

Structure of the potato inhibitor complex of carboxypeptidase A at 2.5-A resolution.

Replication of the plasmid pBR322 under the control of a cloned replication origin from the single-stranded DNA phage M13.

Hydrophobic basis of packing in globular proteins.

Developmental shifts in frequency distribution of polysomal mRNA and their posttranscriptional regulation in the sea urchin embryo.

Iminobiotin affinity columns and their application to retrieval of streptavidin.

Electron microscope study of duck globin mRNA precursor crosslinked in situ.

Anchorage of a band 3 population at the erythrocyte cytoplasmic membrane surface: protein rotational diffusion measurements.

Can the binding of Ca2+ to two regulatory sites on troponin C determine the steep pCa/tension relationship of skeletal muscle?

Location of the chromophore in bacteriorhodopsin.

Regulation of the production of secretory proteins: intracellular degradation of newly synthesized 'defective' collagen.

Nerve growth factor receptors on PC12 cells: ligand-induced conversion from low- to high-affinity states.

Long-term culture of human bone marrow cells.

Cell density determines epithelial migration in culture.

Inhibition of Ca2+ accumulation in mitogen-activated lymphocytes: role of membrane-bound plasma lipoproteins.

Sertoli cells contain a mitogenic polypeptide.

In utero analysis of sister chromatid exchange: alterations in suscptibility to mutagenic damage as a function of fetal cell type and gestational age.

The cbpA gene: role of the 26,000-dalton carbohydrate-binding protein in intercellular cohesion of developing Dictyostelium discoideum cells.

Differential processing of subunits of human chorionic gonadotropin by granulosa cells in vivo.

Bioenergetic aspects and polymer length distribution in steady-state head-to-tail polymerization of actin or microtubules.

High molecular weight polypeptides (270,000-340,000) from cultured cells are related to hog brain microtubule-associated proteins but copurify with intermediate filaments.

Cycle-related toxicity and transformation in 10T1/2 cells treated with N-methyl-N'-nitro-N-nitrosoguanidine.

Composition of native and reconstituted chromatin particles: direct mass determination by scanning transmission electron microscopy.

Crosslinkage and visualization of acetylcholine receptors on myotubes with biotinylated alpha-bungarotoxin and fluorescent avidin.

Talpid2 mutant of the chicken with perturbed cartilage development has an altered precartilage-specific chromatin protein.

A cell surface molecule involved in aggregation of embryonic liver cells.

Linkage of alpha G-Philadelphia to alpha-thalassemia in African-Americans .

Inactive X chromosome DNA does not function in DNA-mediated cell transformation for the hypoxanthine phosphoribosyltransferase gene.

Mouse peritoneal macrophages release leukotriene C in response to a phagocytic stimulus.

Radiometric analysis of biological oxidations in man: sex differences in estradiol metabolism.

Fecalase: a model for activation of dietary glycosides to mutagens by intestinal flora.

Improving the anti-tumor activity of 5-fluorouracil by increasing its incorporation into RNA via metabolic modulation.

Isolation of BPgp70, a fibroblast receptor for the envelope antigen of Rauscher murine leukemia virus.

Retinal rhythms in chicks: circadian variation in melantonin and serotonin N-acetyltransferase activity.

Enkephalin biosynthetic pathway: proteins of 8000 and 14,000 daltons in bovine adrenal medulla.

Animal model for immune dysfunction associated with adenosine deaminase deficiency.

Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood.

On the process of cellular division in Escherichia coli: isolation and characterization of penicillin-binding proteins 1a, 1b, and 3.

In vitro synthesis of repressible yeast acid phosphatase: identification of multiple mRNAs and products.

Yeast fatty acid synthetase: Structure—function relationship and nature of the β-ketoacyl synthetase site

Primary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12.

Induction of ornithine decarboxylase by renin-free nerve growth factor.

Procoat, the precursor of M13 coat protein, requires an electrochemical potential for membrane insertion.

Differences in organizational structure of insulin receptor on rat adipocyte and liver plasma membranes: role of disulfide bonds.

Neutral protease activation of peritoneal macrophage prostaglandin synthesis.

Microtubule-associated proteins: a monoclonal antibody to MAP2 binds to differentiated neurons.

Association of microtubules and intermediate filaments in chicken gizzard cells as detected by double immunofluorescence.

Fluorescence immunoassay based on long time correlations of number fluctuations.

Activation of membrane-bound kallikrein and renin in the kidney.

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Cotransfer of circular and linear prokaryotic and eukaryotic DNA sequences into mouse cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Forget, B. G. Warrick, H. Deriel, J. K. Skoultchi, A. Tuan, D. Hsiung, N. Kucherlapati, R.
Abstract	We have attempted to introduce some eukaryotic and prokaryotic DNA sequences into mouse fibroblasts. Purified herpes thymidine kinase gene (tk) was introduced into mouse cells. The presence of the herpes tk gene was established by gel electrophoresis, sensitivity to the purine analog acyloguanosine, and Southern blot hybridization. We utilized two different methods to introduce nonselectable markers into mouse cells. Bacterial plasmid pBR322 was ligated to herpes tk and used for transfection. All cells that were TK+ also contained the plasmid sequences. In the second method, pBR322 DNA was mixed with herpes tk DNA and presented to mouse cells. TK+ cells were tested for pBR322 sequences by blot hydridization. The frequency of unlinked cotransfer was greater than 40%. When the circular plasmid containing pBR322 and tk was used for transfection, each of the resulting transfectants acquired several copies of the plasmid. Most of the copies were associated with high molecular weight DNA in the cell. In addition, we found that some of the plasmid molecules may exist as free circular molecules. Using the nonligated cotransfer method, we introduced purified human beta-globin sequences into the recipient cells. We were unable to detect any transcripts of the human beta-globin gene at a level greater than or equal to 10 molecules per cell.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	8
Volume Number	77
Language	English
Publisher	National Academy of Sciences
Publisher Date	1980-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	DNA, Circular Genetics Transfection DNA, Viral Genes Globins L Cells (Cell Line) Physiology Molecular Weight Simplexvirus Thymidine Kinase Transcription, Genetic Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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