NDLI: Molecular cloning and characterization of winter flounder antifreeze cDNA.

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Molecular cloning and characterization of winter flounder antifreeze cDNA.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Gross, J. K. Lin, Y.
Abstract	Double-stranded cDNA was synthesized from partially purified winter flounder antifreeze mRNA and inserted into the endonuclease Pst I site of plasmid pBR322 by the poly(dG).poly(dC) homopolymer extension technique. The recombinant plasmids wee used to transform Escherichia coli. Clones containing antifreeze cDNA inserts were identified by the hybridization-selection technique. One of the inserts, 380 nucleotides in length, was digested with endonucleases Sau3AI and HinfI, which cleaved the insert into three fragments. The nucleotide sequences of these fragments were determined. The cDNA contains the entire coding sequence for a possible antifreeze peptide, including the leader sequence. The predicted amino acid sequence is similar to but not identical to one of the known sequences of antifreeze peptide. Within the cDNA are three segments of repeating sequences. The basic repeating sequence of 11 amino acids is maintained in the amino acid sequence coded by the cDNA and in the antifreeze peptide.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	5
Volume Number	78
Language	English
Publisher	National Academy of Sciences
Publisher Date	1981-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cloning, Molecular DNA, Recombinant Metabolism Glycoproteins Genetics Amino Acid Sequence Animals Antifreeze Proteins DNA Restriction Enzymes DNA, Single-Stranded Escherichia Coli Freezing Nucleic Acid Hybridization Plasmids Protein Biosynthesis RNA, Messenger Transcription, Genetic Transformation, Bacterial Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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