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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Saxton, R. E. Irie, R. F. Sze, L. L. |
| Abstract | We established two long-term human B-lymphoblastoid cell lines (L55 and L72) transformed by Epstein-Barr virus that produced IgM kappa antibodies to the human tumor antigen, OFA-I. Periphral blood lymphocytes obtained from melanoma patients were used as the source of the B lymphocytes. Antibody specificity was determined by the immune adherence assay using various human cancer and noncancer tissues as targets. L55 antibody (designated anti-OFA-I-1) reacted with a variety of human tumor types whereas L72 antibody (designated anti-OFA-I-2) reacted only with tumor cells of neuroectodermal origin (melanoma, glioma, and neuroblastoma). The levels of IgM detected in the spent medium of 1 X 10(6) L55 and L72 cells were 4 and 9 micrograms/ml, respectively, by radioimmunoassay. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 18 |
| Volume Number | 79 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1982-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Antibody Formation Antigens, Neoplasm Genetics B-Lymphocytes Immunology Burkitt Lymphoma Microbiology Cell Transformation, Neoplastic Herpesvirus 4, Human Melanoma Cell Line Immunoglobulin M Immunoglobulin Kappa-Chains Neoplasms Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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