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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sahasrabuddhe, C. Lachman, L. Maizel, A. Mehta, S. Morgan, J. Ford, R. |
| Abstract | Recent studies have established the ability of human B lymphocytes to undergo G1-phase cell cycle progression and subsequent DNA synthesis upon exposure to factor(s) present in media conditioned by lectin-stimulated mononuclear cells. Procedures for the isolation of such a cytokine have been the focus of the present investigation. Conditioned medium from cells stimulated by lectin for 72 hr was fractionated by ammonium sulfate precipitation, ion-exchange chromatography, and gel filtration chromatography. During the isolation procedure the proliferation-stimulating activity of the column fractions was assayed concurrently on purified human T cells, purified human B cells, and murine thymocytes. T cell and B cell stimulatory factors present in the initial conditioned medium were found to copurify during ammonium sulfate precipitation, DEAE-Sephadex chromatography, and Bio-Gel P-30 gel filtration. However, partial separation of these two activities was achieved after Bio-Gel P-100 gel filtration. Analytic polyacrylamide gel electrophoresis of radiolabeled Bio-Gel P-100 column fractions demonstrated a distinct protein band of 14,000-15,000 daltons in those column fractions predominantly supporting T cell growth and a distinct protein band of 12,000-13,000 daltons for those fractions predominantly supporting B cell growth. The fractions associated with B cell mitogenic activity induced B cell S-phase entry in a proportion of B lymphocytes in the absence of any detectable IgM secretion. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 19 |
| Volume Number | 79 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1982-01-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | B-Lymphocytes Immunology Growth Substances Isolation & Purification Cell Cycle Cell Separation Cells, Cultured DNA Replication Immunoglobulin M Genetics Interleukin-4 Kinetics Lymphocyte Activation Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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