NDLI: Evidence for a substrate cycle between AMP and adenosine in isolated hepatocytes.

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Evidence for a substrate cycle between AMP and adenosine in isolated hepatocytes.

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Evidence for a substrate cycle between AMP and adenosine in isolated hepatocytes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bontemps, F. Van Den Berghe, G. Hers, H. G.
Abstract	The effect of adenosine on the metabolism of prelabeled adenine nucleotides was investigated in isolated hepatocytes. Adenosine caused an approximately equal to 2-fold increase in the ATP content of the cells. This effect was in part counteracted by an increased rate of adenine nucleotide catabolism that could be explained by a stimulation of both AMP deaminase (AMP aminohydrolase, EC 3.5.4.6) and the cytoplasmic 5'-nucleotidase (5'-ribonucleotide phosphohydrolase, EC 3.1.3.5) because of the increased concentration of ATP. The unexpected finding that labeled adenosine was formed immediately after the addition of the unlabeled nucleoside could be explained by the trapping effect of adenosine. An accumulation of labeled adenosine was observed also in the presence of 5-iodotubercidin, a potent inhibitor of adenosine kinase (ATP:adenosine 5'-phosphotransferase, EC 2.7.1.20). Under these conditions, there was a decrease in the concentration of ATP in the cell and a 2- to 3-fold increase in the rate of formation of allantoin. This formation of adenosine was only slightly decreased by inhibition of the membranous 5'-nucleotidase; it led to the accumulation of S-adenosylhomocysteine in the presence of coformycin and an excess of L-homocysteine. It was concluded that, under basal conditions, the cytoplasmic 5'-nucleotidase present in the liver cell continuously produces adenosine, which is immediately reconverted into AMP by adenosine kinase, without giving rise to allantoin. This futile cycle between AMP and adenosine amounts to at least 20 nmol/min per g of liver and, thus, exceeds the basic rate of allantoin formation.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	10
Volume Number	80
Language	English
Publisher	National Academy of Sciences
Publisher Date	1983-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Adenine Nucleotides Metabolism Adenosine Monophosphate Adenosine Liver AMP Deaminase Adenosine Kinase Animals Nucleotidases Phosphoric Monoester Hydrolases S-Adenosylhomocysteine Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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