NDLI: Isolation of sea urchin egg microtubules with taxol and identification of mitotic spindle microtubule-associated proteins with monoclonal antibodies.

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Initiation of DNA replication in vitro by a DNA-membrane complex extracted from Bacillus subtilis.

Isolation of sea urchin egg microtubules with taxol and identification of mitotic spindle microtubule-associated proteins with monoclonal antibodies.

Characterization of monoclonal antibodies against mitochondrial F1-ATPase.

Processing of transcription products of the gene encoding the RNA component of RNase P.

Intermediate-size filaments in a germ cell: Expression of cytokeratins in oocytes and eggs of the frog Xenopus.

Structural analysis of antigen-specific Ia-bearing regulatory T-cell factors: gel electrophoretic analysis of the antigen-specific augmenting T -cell factor.

Dissecting the antigenic mosaic of the Archaebacterium Methanobacterium thermoautotrophicum by monoclonal antibodies of defined molecular specificity.

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Analysis of HLA class I genes with restriction endonuclease fragments: implications for polymorphism of the human major histocompatibility complex.

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Nanosecond flash photolysis study of carbon monoxide binding to the beta chain of hemoglobin Zürich [beta 63(E7)His leads to Arg].

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Chromosomal mapping of the mink cell focus-inducing and xenotropic env gene family in the mouse.

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Age-associated impairment of murine natural killer activity.

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Isolation of sea urchin egg microtubules with taxol and identification of mitotic spindle microtubule-associated proteins with monoclonal antibodies.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Vallee, R. B. Bloom, G. S.
Abstract	Microtubules were isolated from unfertilized eggs of the sea urchin with the use of the anti-tumor drug taxol. In addition to tubulin, prominent high molecular weight (Mr 205,000-350,000) microtubule-associated proteins (MAPs) were identified as well as MAP species of Mrs 77,000, 100,000, and 120,000. The microtubules were covered with both short periodic arms and longer filamentous arms, both classes of which appeared to crosslink the microtubules into bundles. Monoclonal antibodies were prepared to an unfractionated MAPs preparation. We isolated clonal hybridoma lines producing antibodies to tubulin and to four non-tubulin proteins of Mrs 235,000, 205,000, 150,000, and 37,000. All antibodies strongly and specifically stained the mitotic spindle of dividing sea urchin eggs. All four of the immunoreactive, non-tubulin species behaved as MAPs during microtubule isolation. Thus, we have identified a variety of sea urchin MAPs by biochemical, ultrastructural, and immunochemical means. The immunochemical experiments demonstrated that four of these proteins are microtubule-associated components of the mitotic spindle. We suggest that those proteins that we observed as cross-bridges between the isolated microtublules may be either structural or functional components of the spindle.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	20
Volume Number	80
Language	English
Publisher	National Academy of Sciences
Publisher Date	1983-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Alkaloids Microtubules Ultrastructure Mitosis Sea Urchins Spindle Apparatus Animals Antibodies, Monoclonal Microscopy, Electron Microtubule-Associated Proteins Molecular Weight Paclitaxel Proteins Immunology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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