NDLI: Isolation of the yeast structural gene for the membrane-associated enzyme phosphatidylserine synthase.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1983 Volume: 80 Issue: 24

Year: 1983 Volume: 80 Issue: 23

Construction and identification of mouse amelogenin cDNA clones.

7S-K nuclear RNA from simian virus 40-transformed cells has sequence homology to the viral early promoter.

Protein complexes of intermediate-sized filaments: melting of cytokeratin complexes in urea reveals different polypeptide separation characteristics.

Cell cycle-dependent intervention by benzamide of carcinogen-induced neoplastic transformation and in vitro poly(ADP-ribosyl)ation of nuclear proteins in human fibroblasts.

Genetic analysis of the folding pathway for the tail spike protein of phage P22.

Mechanisms of integration of de novo-synthesized polypeptides into membranes: signal-recognition particle is required for integration into microsomal membranes of calcium ATPase and of lens MP26 but not of cytochrome b5.

A frameshift mutation affecting the carboxyl terminus of the simian virus 40 large tumor antigen results in a replication- and transformation-defective virus.

Antibodies to the Epstein-Barr virus nuclear antigen and to rheumatoid arthritis nuclear antigen identify the same polypeptide.

Activation of the c-mos oncogene in a mouse plasmacytoma by insertion of an endogenous intracisternal A-particle genome.

Identification of a cytoplasmic tropomyosin gene linked to two muscle tropomyosin genes in Drosophila.

Identification of a primosome assembly site in the region of the ori 2 replication origin of the Escherichia coli mini-F plasmid.

Structure and expression of the dnaQ mutator and the RNase H genes of Escherichia coli: overlap of the promoter regions.

beta-Adrenergic receptor regulation of N-linked protein glycosylation in rat parotid acinar cells.

Phorbol esters, phospholipase C, and growth factors rapidly stimulate the phosphorylation of a Mr 80,000 protein in intact quiescent 3T3 cells.

Yeast plasmid 2-micron circle promotes recombination within bacterial transposon Tn5.

DNA sequences near the site of reciprocal recombination between a c-myc oncogene and an immunoglobulin switch region.

Directional transport and integration of donor DNA in Haemophilus influenzae transformation.

Isolation of the yeast structural gene for the membrane-associated enzyme phosphatidylserine synthase.

Site-specific recombination of yeast 2-micron DNA in vitro.

Sequences essential for transposition at the termini of IS50.

Point mutations and DNA rearrangements 5' to the inducible qa-2 gene of Neurospora allow activator protein-independent transcription.

Construction and testing of mouse--human heteromyelomas for human monoclonal antibody production.

Exon-intron organization and complete nucleotide sequence of a human major histocompatibility antigen DC beta gene.

Detection of lymphocytes producing a human retrovirus associated with adult T-cell leukemia by syncytia induction assay.

Associative long-term potentiation in hippocampal slices.

Metorphamide: isolation, structure, and biologic activity of an amidated opioid octapeptide from bovine brain.

An MF alpha 1-SUC2 (alpha-factor-invertase) gene fusion for study of protein localization and gene expression in yeast.

Identification of the epsilon-subunit of Escherichia coli DNA polymerase III holoenzyme as the dnaQ gene product: a fidelity subunit for DNA replication.

Protease-sensitive regions in myosin subfragment 1.

Kinetic characterization and regulation of phosphoenolpyruvate-dependent methyl alpha-D-glucopyranoside transport by Salmonella typhimurium membrane vesicles.

Polarization of the Golgi apparatus and the microtubule-organizing center within cloned natural killer cells bound to their targets.

Transformation of retinal glia cells into lens phenotype: expression of MP26, a lens plasma membrane antigen.

A synaptic vesicle antigen is restricted to the junctional region of the presynaptic plasma membrane.

Tunicamycin inhibits ganglioside biosynthesis in rat liver Golgi apparatus by blocking sugar nucleotide transport across the membrane vesicles.

Transient expression of genes introduced into cultured cells of Drosophila.

Modification of glycogen synthase activity in isolated rat hepatocytes by tumor-promoting phorbol esters: evidence for differential regulation of glycogen synthase and phosphorylase.

Intracellular calcium measurements by 19F NMR of fluorine-labeled chelators.

Arachidonate metabolism by human polymorphonuclear leukocytes stimulated by N-formyl-Met-Leu-Phe or complement component C5a is independent of phospholipase activation.

Structure-specific model of hemoglobin cooperativity.

A random-walk model for helix bending in B-DNA.

Targeted and nontargeted liposomes for in vivo transfer to rat liver cells of a plasmid containing the preproinsulin I gene.

Crystallographic studies on apocarboxypeptidase A and the complex with glycyl-L-tyrosine.

Construction and characterization of an infectious vaccinia virus recombinant that expresses the influenza hemagglutinin gene and induces resistance to influenza virus infection in hamsters.

Oxidation of methionine residues in proteins of activated human neutrophils.

3-Hydroxy-3-methylglutaryl-CoA reductase: a transmembrane glycoprotein of the endoplasmic reticulum with N-linked 'high-mannose' oligosaccharides.

Translational diffusion in phospholipid monolayers measured by fluorescence microphotolysis.

Phospholipid domains in bovine retinal rod outer segment disk membranes.

Deuterium NMR as a structural probe for micelle-associated carbohydrates: D-mannose.

Laser-induced multiphoton processes in living cells.

Synthetic leader peptide modulates secretion of proteins from microinjected Xenopus oocytes.

Beta-adrenergic stimulation of Ca2+ fluxes, endocytosis, hexose transport, and amino acid transport in mouse kidney cortex is mediated by polyamine synthesis.

Nerve growth factor: stimulation of polymorphonuclear leukocyte chemotaxis in vitro.

Reciprocal modulation of growth and differentiated functions of mature rat hepatocytes in primary culture by cell--cell contact and cell membranes.

Steady-state theory of the interference of GTP hydrolysis in the mechanism of microtubule assembly.

Amplified C lambda and c-abl genes are on the same marker chromosome in K562 leukemia cells.

Lysis of Chinese hamster embryo fibroblast mutants by human natural cytotoxic (NK) cells.

Fibrinogen Baltimore II: congenital hypodysfibrinogenemia with delayed release of fibrinopeptide B and decreased rate of fibrinogen synthesis.

Pseudo arylsulfatase-A deficiency in healthy individuals: genetic and biochemical relationship to metachromatic leukodystrophy.

Experimental tumoricidal effects of monoclonal antibody against solid breast tumors.

De novo purine synthesis in cultured rat embryos undergoing organogenesis.

Brain-blood barrier? Yes and no.

Biochemical and immunochemical evidence that the 'major postsynaptic density protein' is a subunit of a calmodulin-dependent protein kinase.

Cellular calcium fluctuations in mammalian heart: direct evidence from noise analysis of aequorin signals in Purkinje fibers.

Surface properties of an amphiphilic peptide hormone and of its analog: corticotropin-releasing factor and sauvagine.

Year: 1983 Volume: 80 Issue: 22

Year: 1983 Volume: 80 Issue: 21

Year: 1983 Volume: 80 Issue: 20

Year: 1983 Volume: 80 Issue: 19

Year: 1983 Volume: 80 Issue: 18

Year: 1983 Volume: 80 Issue: 17

Year: 1983 Volume: 80 Issue: 16

Year: 1983 Volume: 80 Issue: 15

Year: 1983 Volume: 80 Issue: 14

Year: 1983 Volume: 80 Issue: 13

Year: 1983 Volume: 80 Issue: 12

Year: 1983 Volume: 80 Issue: 11

Year: 1983 Volume: 80 Issue: 10

Year: 1983 Volume: 80 Issue: 9

Year: 1983 Volume: 80 Issue: 8

Year: 1983 Volume: 80 Issue: 7

Year: 1983 Volume: 80 Issue: 6

Year: 1983 Volume: 80 Issue: 5

Year: 1983 Volume: 80 Issue: 4

Year: 1983 Volume: 80 Issue: 3

Year: 1983 Volume: 80 Issue: 2

Year: 1983 Volume: 80 Issue: 1

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Isolation of the yeast structural gene for the membrane-associated enzyme phosphatidylserine synthase.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Letts, V. A. Carman, G. M. Henry, S. A. Klig, L. S. Bae-lee, M.
Abstract	The structural gene (CHO1) for phosphatidylserine synthase (CDPdiacylglycerol:L-serine O-phosphatidyltransferase, EC 2.7.8.8) was isolated by genetic complementation in Saccharomyces cerevisiae from a bank of yeast genomic DNA on a chimeric plasmid. The cloned DNA (4.0 kilobases long) was shown to represent a unique sequence in the yeast genome. The DNA sequence on an integrative plasmid was shown to recombine into the CHO1 locus, confirming its genetic identity. The cho1 yeast strain transformed with this gene on an autonomously replicating plasmid had significantly increased activity of the regulated membrane-associated enzyme phosphatidylserine synthase. Partial purification of phosphatidylserine synthase from microsomes of this transformed strain confirmed that the membrane-bound enzyme was overproduced 6- to 7-fold as compared with the wild-type strain. The strain also synthesized the product phospholipid, phosphatidylserine, at an increased rate. The transformed strain had altered proportions of a variety of other phospholipids, suggesting that their synthesis is affected by the rate of synthesis of phosphatidylserine in yeast.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	23
Volume Number	80
Language	English
Publisher	National Academy of Sciences
Publisher Date	1983-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	CDPdiacylglycerol-Serine O-Phosphatidyltransferase Genetics Cloning, Molecular DNA, Fungal Isolation & Purification Genes, Fungal Genes Phosphotransferases Saccharomyces Cerevisiae Enzymology Cell Membrane Crosses, Genetic DNA Restriction Enzymes Phenotype Phospholipids Biosynthesis Plasmids Species Specificity Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in