NDLI: Purification of bovine bone morphogenetic protein by hydroxyapatite chromatography.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1984 Volume: 81 Issue: 24

Year: 1984 Volume: 81 Issue: 23

Year: 1984 Volume: 81 Issue: 22

Year: 1984 Volume: 81 Issue: 21

Year: 1984 Volume: 81 Issue: 20

Year: 1984 Volume: 81 Issue: 19

Year: 1984 Volume: 81 Issue: 18

Year: 1984 Volume: 81 Issue: 17

Year: 1984 Volume: 81 Issue: 16

Year: 1984 Volume: 81 Issue: 15

Year: 1984 Volume: 81 Issue: 14

Year: 1984 Volume: 81 Issue: 13

Year: 1984 Volume: 81 Issue: 12

Year: 1984 Volume: 81 Issue: 11

Year: 1984 Volume: 81 Issue: 10

Year: 1984 Volume: 81 Issue: 9

Year: 1984 Volume: 81 Issue: 8

Year: 1984 Volume: 81 Issue: 7

Year: 1984 Volume: 81 Issue: 6

Year: 1984 Volume: 81 Issue: 5

Year: 1984 Volume: 81 Issue: 4

Year: 1984 Volume: 81 Issue: 3

Year: 1984 Volume: 81 Issue: 2

Free cytoplasmic Ca2+ and neurotransmitter release: studies on PC12 cells and synaptosomes exposed to alpha-latrotoxin.

Specific covalent binding of platelet-derived growth factor to human plasma alpha 2-macroglobulin.

Association of cytokeratin p39 with DNA in intact Novikoff hepatoma cells.

Mutational analysis of a regulatory region in bacteriophage lambda that has overlapping signals for the initiation of transcription and translation.

Determination of the promoter region of mouse ribosomal RNA gene by an in vitro transcription system.

Stimulation of in vitro transcription from heterologous promoters by the simian virus 40 enhancer.

Single catalytic site model for the oxidation of ferrocytochrome c by mitochondrial cytochrome c oxidase.

Mechanism of translational control by partial phosphorylation of the alpha subunit of eukaryotic initiation factor 2.

Avian sarcoma virus gag and env gene structural protein precursors contain a common amino-terminal sequence.

Regulated expression of a human interferon gene in yeast: control by phosphate concentration or temperature.

Purification of bovine bone morphogenetic protein by hydroxyapatite chromatography.

H2-uptake activity of the MoFe protein component of Azotobacter vinelandii nitrogenase.

Chimeric influenza virus hemagglutinin containing either the NH2 terminus or the COOH terminus of G protein of vesicular stomatitis virus is defective in transport to the cell surface.

Direct isolation of the functional human thymidine kinase gene with a cosmid shuttle vector.

Cytosolic calcium regulates epidermal growth factor endocytosis in rat pancreas and cultured fibroblasts.

Rapid release of galactose-terminated ligands after endocytosis by hepatic parenchymal cells: evidence for a role of carbohydrate structure in the release of internalized ligand from receptor.

Von Willebrand protein binds to extracellular matrices independently of collagen.

Regional localization on the human X chromosome and polymorphism of the coagulation factor IX gene (hemophilia B locus).

Correction of complex heteroduplexes made of mouse H-2 gene sequences in Escherichia coli K-12.

Chromosome assignments of four mouse cellular homologs of sarcoma and leukemia virus oncogenes.

In vivo formation of gene fusions encoding hybrid beta-galactosidase proteins in one step with a transposable Mu-lac transducing phage.

Escherichia coli mutants suppressing replication-defective mutations of the ColE1 plasmid.

Identification of thymidine nucleotidase and deoxyribonucleotidase activities among normal isozymes of 5'-nucleotidase in human erythrocytes.

Injection of mice with antibody to interferon renders peritoneal macrophages permissive for vesicular stomatitis virus and encephalomyocarditis virus.

Competitive inhibition by diacylglycerol of specific phorbol ester binding.

Activation of Ca-dependent K channels by carbamoylcholine in rat lacrimal glands.

Rod light response augmented by active phosphodiesterase.

Na+-H+ exchange activity in renal brush border membrane vesicles in response to metabolic acidosis: The role of glucocorticoids.

Nuclease protection analysis of ribonucleoprotein complexes: use of the cytotoxic ribonuclease alpha-sarcin to determine the binding sites for Escherichia coli ribosomal proteins L5, L18, and L25 on 5S rRNA.

Primary structure and transcription of an amplified genetic locus: the CUP1 locus of yeast.

tRNAMetf2 gene in the leader region of the nusA operon in Escherichia coli.

Protein HU in the enzymatic replication of the chromosomal origin of Escherichia coli.

Pronounced structural similarities between the small subunit ribosomal RNA genes of wheat mitochondria and Escherichia coli.

Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis.

Localization of the structural gene for human apolipoprotein A-I on the long arm of human chromosome 11.

Homoeosis in Drosophila: the ultrabithorax larval syndrome.

Antithrombin III Toyama: replacement of arginine-47 by cysteine in hereditary abnormal antithrombin III that lacks heparin-binding ability.

Frequent generation of nonrescuable reorganized Moloney murine sarcoma viral genomes.

Nuclear matrix DNA from chicken erythrocytes contains beta-globin gene sequences.

Cloning of a cDNA encoding rat intestinal fatty acid binding protein.

Ionizing radiation and tritium transmutation both cause formation of 5-hydroxymethyl-2'-deoxyuridine in cellular DNA.

Trifluoperazine inhibits insulin action on glucose metabolism in fat cells without affecting inhibition of lipolysis.

The possibility that the spectrum of intermediate two, seen in the course of reaction of flavoenzyme phenol hydroxylases, may be attributable to iminol isomers of a flavin-derived 6-arylamino-5-oxo(3H,5H)uracil.

Purification and characterization of acidic fibroblast growth factor from bovine brain.

Xenopsin: the neurotensin-like octapeptide from Xenopus skin at the carboxyl terminus of its precursor.

Glycosylated ovine prolactin.

Single-chain structure of human ceruloplasmin: the complete amino acid sequence of the whole molecule.

Microinjected pBR322 stimulates cellular DNA synthesis in Swiss 3T3 cells.

Preferential binding of estrogen-receptor complex to a region containing the estrogen-dependent hypomethylation site preceding the chicken vitellogenin II gene.

Cell cycle regulation of human histone H1 mRNA.

Dynamics of enzymatic reactions.

Plasma membrane reorganization induced by tumor promoters in an epithelial cell line.

Long-term cultivation and differentiation of human erythroleukemia cells in a protein-free chemically defined medium.

Asymmetric acetylcholinesterase is assembled in the Golgi apparatus.

On the mechanism of nucleolar dominance in mouse-human somatic cell hybrids.

Amounts, synthesis, and some properties of intracisternal A particle-related RNA in early mouse embryos.

Structural intermediates of deletion mutagenesis: a role for palindromic DNA.

Assignment of the genes for the alpha and beta subunits of thyrotropin to different mouse chromosomes.

Molecular hybridization under conditions of high stringency permits cloned DNA segments containing reiterated DNA sequences to be assigned to specific chromosomal locations.

Molecular cloning and expression of the mouse ornithine decarboxylase gene.

Neutron-capture therapy of human cancer: in vivo results on tumor localization of boron-10-labeled antibodies to carcinoembryonic antigen in the GW-39 tumor model system.

Hepatitis B surface antigen and polymerized albumin binding activity in sheep serum.

Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.

A bombesin immunoreactive peptide in milk.

Accelerated age-related browning of human collagen in diabetes mellitus.

Regression of myelocytic leukemia in rats after hypophysectomy.

Neuropeptide-induced contraction and relaxation of the mouse anococcygeus muscle.

Changes in local cerebral glucose utilization during rewarding brain stimulation.

Determinants for protein localization: beta-lactamase signal sequence directs globin across microsomal membranes.

Immunoprecipitation of cell surface structures of cloned cytotoxic T lymphocytes by clone-specific antisera.

Immunoglobulin gene rearrangement as a diagnostic criterion of B-cell lymphoma.

Year: 1984 Volume: 81 Issue: 1

Retinal migration during dark reduction of bacteriorhodopsin.

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Purification of bovine bone morphogenetic protein by hydroxyapatite chromatography.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Tempst, P. Huo, Y. K. Urist, M. R. Lietze, A. Buyske, J. Delange, R. J. Hohl, W. M. Brownell, A. G. Hunkapiller, M.
Abstract	Bovine bone morphogenetic protein (bBMP) induces differentiation of mesenchymal-type cells into cartilage and bone. bBMP has an apparent Mr of 18,500 +/- 500 and represents less than 0.001% of the wet weight of bone tissue. A Mr 34,000 protein resembling osteonectin is separated by extraction with Triton X-100. A Mr 24,000 protein and about half of a Mr 22,000 protein are disassociated from bBMP by precipitation in 1.5 M guanidine hydrochloride. Aggregates of bBMP and a Mr 14,000 protein are insoluble in aqueous media; the bBMP becomes soluble when the Mr 14,000 protein is disassociated in 6 M urea and removed from the solution by ultrafiltration. Three separate molecular species with apparent Mrs 18,500, 17,500, and 17,000 are eluted at 0.10, 0.15, and 0.20 M phosphate ion concentrations, respectively, from a hydroxy-apatite column. The Mr 18,500 protein has the amino acid composition of acidic polylpeptide and includes four half-cystine residues; the pI is 4.9-5.1. The Mr 22,000 component is a chromoprotein resembling ferritin. The NH2-terminal amino acid sequence of the Mr 17,500 protein simulates histone H2B. The Mr 17,000 protein may possess calmodulin activity. Aggregates of the Mr 18,500 and other proteins induce formation of large deposits of bone; the Mr 18,500 protein alone is rapidly absorbed and induces formation of small deposits. None of the other proteins induces bone formation.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	2
Volume Number	81
Language	English
Publisher	National Academy of Sciences
Publisher Date	1984-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Bone And Bones Growth Substances Isolation & Purification Proteins Amino Acid Sequence Amino Acids Animals Biological Assay Bone Morphogenetic Proteins Chromatography, Affinity Durapatite Hydroxyapatites Isoelectric Point Molecular Weight Solubility Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in