NDLI: Specific binding of erythropoietin to spleen cells infected with the anemia strain of Friend virus.

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Specific binding of erythropoietin to spleen cells infected with the anemia strain of Friend virus.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Goldwasser, E. Krantz, S. B.
Abstract	Tritiated erythropoietin with full biological activity has been prepared, and a relatively homogeneous population of enriched progenitor cells that respond to the hormone has been generated by infection of mice with the Friend virus that produces anemia. These cells, obtained from the spleens of infected mice, develop into mature erythroblasts and erythrocytes in the presence of erythropoietin. We have measured the binding of erythropoietin to these target cells; 62% of the binding was inhibited by excess unlabeled erythropoietin, but no inhibition occurred with albumin, serum, or a variety of growth factors and glycoproteins. Apparent equilibrium was reached by 2 hr at 37 degrees C and by 3.5-4 hr at 10 degrees C. The extent of specific binding increased linearly with cell concentration. In binding experiments at 10 degrees C, apparent saturation of specific binding occurred at approximately equal to 8.7 nM. Scatchard analysis showed a single class of binding sites. The dissociation constant is 5.2 nM with an average of 660 binding sites per cell. At 0.06 nM, where most of the cells are induced to terminally differentiate in vitro, an average of only 8 erythropoietin molecules bound per cell. These studies indicate that erythropoietin attaches to specific binding sites, which are most likely receptors since they manifest high affinity and specificity, and that the biologic effect of the hormone may be produced by attachment of a very small number of erythropoietin molecules.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	23
Volume Number	81
Language	English
Publisher	National Academy of Sciences
Publisher Date	1984-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Transformation, Neoplastic Erythropoietin Metabolism Friend Murine Leukemia Virus Genetics Receptors, Cell Surface Spleen Anemia Microbiology Animals Isolation & Purification Kinetics Mice Mice, Inbred BALB C Receptors, Erythropoietin Tritium Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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