NDLI: Acidic pH requirement for insertion of colicin E1 into artificial membrane vesicles: relevance to the mechanism of action of colicins and certain toxins.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1985 Volume: 82 Issue: 24

Year: 1985 Volume: 82 Issue: 23

Year: 1985 Volume: 82 Issue: 22

Year: 1985 Volume: 82 Issue: 21

Year: 1985 Volume: 82 Issue: 20

Year: 1985 Volume: 82 Issue: 19

Year: 1985 Volume: 82 Issue: 18

Year: 1985 Volume: 82 Issue: 17

Year: 1985 Volume: 82 Issue: 16

Year: 1985 Volume: 82 Issue: 15

Year: 1985 Volume: 82 Issue: 14

Year: 1985 Volume: 82 Issue: 13

Year: 1985 Volume: 82 Issue: 12

Year: 1985 Volume: 82 Issue: 11

Year: 1985 Volume: 82 Issue: 10

Year: 1985 Volume: 82 Issue: 9

Year: 1985 Volume: 82 Issue: 8

Year: 1985 Volume: 82 Issue: 7

Year: 1985 Volume: 82 Issue: 6

Year: 1985 Volume: 82 Issue: 5

Cyclization of RNA 3'-terminal phosphate by cyclase from HeLa cells proceeds via formation of N(3')pp(5')A activated intermediate.

Acidic pH requirement for insertion of colicin E1 into artificial membrane vesicles: relevance to the mechanism of action of colicins and certain toxins.

Treatment of mice with 5-azacytidine efficiently activates silent retroviral genomes in different tissues.

Analysis of an evolutionarily conserved antigenic site on mammalian cytochrome c using synthetic peptides.

Purified hydrophobic proteins, chargerins, are essential for energy transduction in oxidative phosphorylation.

Oxygen chemoreception by carotid body cells in culture.

Transfer RNA is an essential component of the ubiquitin- and ATP-dependent proteolytic system.

Stimulation of glucose uptake by transforming growth factor beta: evidence for the requirement of epidermal growth factor-receptor activation.

Regulated expression of endonuclease EcoRI in Saccharomyces cerevisiae: nuclear entry and biological consequences.

An adenovirus vector system used to express polyoma virus tumor antigens.

Similarity between the corticotropin (ACTH) receptor and a peptide encoded by an RNA that is complementary to ACTH mRNA.

The molecular origin of DNA-drug specificity in netropsin and distamycin.

Mapping the location of psoralen crosslinks on RNA by mung bean nuclease sensitivity of RNA.DNA hybrids.

Characterization of the activation of Na+/H+ exchange in lymphocytes by phorbol esters: change in cytoplasmic pH dependence of the antiport.

Loss of polymorphic restriction fragments in malignant melanoma: implications for tumor heterogeneity.

gp140, the C3d receptor of human B lymphocytes, is also the Epstein-Barr virus receptor.

Characterization of a Q subregion gene in the murine major histocompatibility complex.

Characterization of a protein, released by the T47D cell line, immunologically related to the major envelope protein of mouse mammary tumor virus.

Leukotriene B5 is formed in human neutrophils after dietary supplementation with icosapentaenoic acid.

GABAB-receptor-activated K+ current in voltage-clamped CA3 pyramidal cells in hippocampal cultures.

Xenogeneic antiserum to soluble products from activated lymphoid cells inhibits interleukin 1-mediated functions in the helper pathway of cytolytic-effector-cell differentiation.

The receptor for immunoglobulin E on rat basophilic leukemia cells: effect of ligand binding on receptor expression.

Radioimmunoassay for 6-D-tryptophan analog of luteinizing hormone-releasing hormone: measurement of serum levels after administration of long-acting microcapsule formulations.

A fidelity assay using 'dideoxy' DNA sequencing: a measurement of sequence dependence and frequency of forming 5-bromouracil X guanine base mispairs.

Laminin binds specifically to sulfated glycolipids.

Exclusive nuclear location of estrogen receptors in Squalus testis.

Recombination in mouse L cells between DNA introduced into cells and homologous chromosomal sequences.

A minimal ribosomal RNA: sequence and secondary structure of the 9S kinetoplast ribosomal RNA from Leishmania tarentolae.

Three-dimensional structure of thaumatin I, an intensely sweet protein.

Effects of fibrinogen-binding tetrapeptides on mechanical properties of fine fibrin clots.

The Ca2+-binding protein parvalbumin: molecular cloning and developmental regulation of mRNA abundance.

Interaction of mouse mammary epithelial cells with collagen substrata: regulation of casein gene expression and secretion.

On the origin of species of fibronectin.

Enzyme-controlled scavenging of ascorbyl and 2,6-dimethoxy-semiquinone free radicals in Ehrlich ascites tumor cells.

Calcium channel antagonists inhibit the acrosome reaction and bind to plasma membranes of sea urchin sperm.

Tissue-specific levels of human glucose-6-phosphate dehydrogenase correlate with methylation of specific sites at the 3' end of the gene.

Characterization of an HLA DR beta pseudogene.

Strong antitumor activities of IgG3 antibodies to a human melanoma-associated ganglioside.

Isolation of a cDNA clone encoding pancreatic polypeptide.

High glucose concentrations partially release hexokinase from inhibition by glucose 6-phosphate.

Nerves in the spine of a sea urchin: a neglected division of the echinoderm nervous system.

Dissociation from albumin: a potentially rate-limiting step in the clearance of substances by the liver.

Stimulation of glycolysis and amino acid uptake in NRK-49F cells by transforming growth factor beta and epidermal growth factor.

Isolation and biochemical characterization of the mammalian reovirus type 3 cell-surface receptor.

Affinity-purified interleukin 2 induces proliferation of large but not small B cells.

Purification and biochemical characterization of human pluripotent hematopoietic colony-stimulating factor.

Chimeric chemosensory transducers of Escherichia coli.

Stereochemical studies of a selenium-containing hydrogenase from Methanococcus vannielii: determination of the absolute configuration of C-5 chirally labeled dihydro-8-hydroxy-5-deazaflavin cofactor.

Detection and characterization of a mouse alpha-spectrin cDNA clone by its expression in Escherichia coli.

Microtubule-associated proteins (MAPs): a monoclonal antibody to MAP 1 decorates microtubules in vitro but stains stress fibers and not microtubules in vivo.

Identification and partial characterization of hepatocyte-stimulating factor from leukemia cell lines: comparison with interleukin 1.

Mutations in direct repeat sequences and in a conserved sequence adjacent to the repeats result in a defective replication origin in plasmid R6K.

Correction of hyperglycemia with phloridzin restores the glucagon response to glucose in insulin-deficient dogs: implications for human diabetes.

Molecular cloning and sequence analysis of a chondroitin sulfate proteoglycan cDNA.

Independent control elements that determine yolk protein gene expression in alternative Drosophila tissues.

Year: 1985 Volume: 82 Issue: 4

Year: 1985 Volume: 82 Issue: 3

Year: 1985 Volume: 82 Issue: 2

Year: 1985 Volume: 82 Issue: 1

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Acidic pH requirement for insertion of colicin E1 into artificial membrane vesicles: relevance to the mechanism of action of colicins and certain toxins.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Brunden, K. R. Davidson, V. L. Cramer, W. A.
Abstract	The channel-forming activity of colicin E1 in artificial membranes is known to increase at low pH values and to have a maximum near pH 4 in such membrane vesicles. The present work demonstrates that this pH dependence of activity can be attributed to membrane binding. Maximal binding of colicin E1 and a more slowly binding channel-forming carboxyl-terminal tryptic peptide occurred at acidic pH values, with the effective pK values for binding equal to 4.6 and less than 4.0, respectively. The binding did not require imposition of a transmembrane potential. Insertion of the tryptic peptide into the membrane was shown by retention of bound [3H]leucine-labeled peptide by vesicles after digestion with protease, as well as by retention of the peptide in salt-washed vesicles. The retention after protease treatment was also used to estimate the amount of carboxyl-terminal peptide inserted into the membrane. Approximately 12 of the 21 leucines present in the carboxyl-terminal peptide were retained after Pronase treatment at pH less than 4. Reversibility of the insertion at low pH values was seen after an alkaline shift of pH to 6.0, resulting in a decrease of the protease-inaccessible fraction of the bound protein. A model is presented describing a mechanism in which protonation of one or more carboxyl residues is necessary for effective binding and insertion into the membrane by the channel-forming domain of colicin E1. This model may also be relevant to the mechanism of membrane insertion by certain toxins.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	5
Volume Number	82
Language	English
Publisher	National Academy of Sciences
Publisher Date	1985-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Colicins Hydrogen-Ion Concentration Ion Channels Liposomes Membrane Potentials Membrane Proteins Peptide Fragments Structure-Activity Relationship Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in