NDLI: Rat Sertoli cells acquire a beta-adrenergic response during primary culture.

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Rat Sertoli cells acquire a beta-adrenergic response during primary culture.

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Rat Sertoli cells acquire a beta-adrenergic response during primary culture.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Perkins, J. P. White, M. G. Kierszenbaum, A. L. Spruill, W. A. Tres, L. L.
Abstract	Two-dimensional polyacrylamide gel electrophoresis and the radioligand (-)-[125I]iodopindolol (125I-Pin) have been used to study isoproterenol-dependent protein phosphorylation and beta-adrenergic receptor availability, respectively, in cultured Sertoli cells and freshly isolated seminiferous tubular segments of sexually immature and mature rats. Sertoli cells prepared from sexually immature rats show progressive 125I-Pin binding in primary cultures that correlates with isoproterenol-induced cell shape changes, redistribution of immunoreactive vimentin, and phosphorylation of this intermediate filament protein. The development of 125I-Pin binding to Sertoli cell lysates is blocked by cycloheximide. Seminiferous tubules do not show significant isoproterenol-dependent vimentin phosphorylation nor 125I-Pin binding. However, vimentin phosphorylation can be induced by follicle-stimulating hormone or a cyclic nucleotide analog. This study stresses the need for correlating pharmacological-induced responses observed in Sertoli cell primary cultures with those in the intact seminiferous tubule.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	7
Volume Number	82
Language	English
Publisher	National Academy of Sciences
Publisher Date	1985-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Receptors, Adrenergic, Beta Sertoli Cells Drug Effects Animals Cells, Cultured Follicle Stimulating Hormone Pharmacology In Vitro Techniques Isoproterenol Phosphorylation Pindolol Analogs & Derivatives Metabolism Proteins Seminiferous Tubules Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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