NDLI: Capacity of RecA protein to bind preferentially to UV lesions and inhibit the editing subunit (epsilon) of DNA polymerase III: a possible mechanism for SOS-induced targeted mutagenesis.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1986 Volume: 83 Issue: 24

Year: 1986 Volume: 83 Issue: 23

Year: 1986 Volume: 83 Issue: 22

Year: 1986 Volume: 83 Issue: 21

Year: 1986 Volume: 83 Issue: 20

Year: 1986 Volume: 83 Issue: 19

Year: 1986 Volume: 83 Issue: 18

Year: 1986 Volume: 83 Issue: 17

Year: 1986 Volume: 83 Issue: 16

Year: 1986 Volume: 83 Issue: 15

Year: 1986 Volume: 83 Issue: 14

Year: 1986 Volume: 83 Issue: 13

Year: 1986 Volume: 83 Issue: 12

Year: 1986 Volume: 83 Issue: 11

Year: 1986 Volume: 83 Issue: 10

Year: 1986 Volume: 83 Issue: 9

Year: 1986 Volume: 83 Issue: 8

Year: 1986 Volume: 83 Issue: 7

Year: 1986 Volume: 83 Issue: 6

Year: 1986 Volume: 83 Issue: 5

Year: 1986 Volume: 83 Issue: 4

Year: 1986 Volume: 83 Issue: 3

RecA protein filaments can juxtapose DNA ends: an activity that may reflect a function in DNA repair.

Effects of the external pH on Ca channels: experimental studies and theoretical considerations using a two-site, two-ion model.

Determinator-inhibitor pairs as a mechanism for threshold setting in development: a possible function for pseudogenes.

Identification of functional regions on the I-Ab molecule by site-directed mutagenesis.

Human T-cell clones used to define functional epitopes on HLA class II molecules.

Increased mRNA for low density lipoprotein receptor in livers of rabbits treated with 17 alpha-ethinyl estradiol.

Three human alcohol dehydrogenase subunits: cDNA structure and molecular and evolutionary divergence.

A genomic clone encoding the alpha chain of the OKM1, LFA-1, and platelet glycoprotein IIb-IIIa molecules.

Increased concentration of spectrin is observed in avian dystrophic muscle.

Involvement of bases 787-795 of Escherichia coli 16S ribosomal RNA in ribosomal subunit association.

A poliovirus temperature-sensitive RNA synthesis mutant located in a noncoding region of the genome.

Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.

Detection of single base-pair mismatches in DNA by chemical modification followed by electrophoresis in 15% polyacrylamide gel.

Sequence-nonspecific replication of transfected plasmid DNA in poxvirus-infected cells.

Mapping of the human APOB gene to chromosome 2p and demonstration of a two-allele restriction fragment length polymorphism.

Monoclonal antibody to the type I insulin-like growth factor (IGF-I) receptor blocks IGF-I receptor-mediated DNA synthesis: clarification of the mitogenic mechanisms of IGF-I and insulin in human skin fibroblasts.

Rates of nuclear DNA evolution in pheasant-like birds: evidence from restriction maps.

A complete library of point substitution mutations in the glucocorticoid response element of mouse mammary tumor virus.

Rapid mapping of antigenic coding regions and constructing insertion mutations in yeast genes by mini-Tn10 'transplason' mutagenesis.

Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae.

Detection of lymphocytes expressing human T-lymphotropic virus type III in lymph nodes and peripheral blood from infected individuals by in situ hybridization.

Cloning and comparison of repeated DNA sequences from the human filarial parasite Brugia malayi and the animal parasite Brugia pahangi.

Expression of the oncogene of avian reticuloendotheliosis virus in Escherichia coli and identification of the transforming protein in reticuloendotheliosis virus T-transformed cells.

Irradiation-resistance conferred by superoxide dismutase: possible adaptive role of a natural polymorphism in Drosophila melanogaster.

Multiplicity of satellite DNA sequences in Drosophila melanogaster.

Molecular cloning, germ-line transformation, and transcriptional analysis of the zeste locus of Drosophila melanogaster.

Multiple purine pathway enzyme activities are encoded at a single genetic locus in Drosophila.

Intracellular transport blockade caused by disruption of the disulfide bridge in the third external domain of major histocompatibility complex class I antigen.

Induction of NAD(P)H:quinone reductase in murine hepatoma cells by phenolic antioxidants, azo dyes, and other chemoprotectors: a model system for the study of anticarcinogens.

On the mechanism of the chemical and enzymic oxygenations of alpha-oxyprotohemin IX to Fe.biliverdin IX alpha.

Transcriptional control elements of hepatitis B surface antigen gene.

Distinctive role of histidine-16 of the B beta chain of fibrinogen in the end-to-end association of fibrin.

Two ornithine decarboxylase mRNA species in mouse kidney arise from size heterogeneity at their 3' termini.

Total synthesis of a gene for bovine rhodopsin.

A purified transcription factor (TIF-IB) binds to essential sequences of the mouse rDNA promoter.

Interaction of vanadate with phenol and tyrosine: implications for the effects of vanadate on systems regulated by tyrosine phosphorylation.

Capacity of RecA protein to bind preferentially to UV lesions and inhibit the editing subunit (epsilon) of DNA polymerase III: a possible mechanism for SOS-induced targeted mutagenesis.

Human HSP70 promoter contains at least two distinct regulatory domains.

Purification and assay of a 145-kDa protein (STOP145) with microtubule-stabilizing and motility behavior.

The nature of enzyme catalysis in trypsin.

Stimulation of aortic smooth muscle cell mitogenesis by serotonin.

Deficiency of adenosine deaminase in the wasted mouse.

Regulation of translocated c-myc genes transfected into plasmacytoma cells.

Developmental and tissue-specific expression directed by the alpha 2 type I collagen promoter in transgenic mice.

Influence of a genetic difference confined to mutation of H-2K on the incidence of pregnancy block in mice.

Murine T-cell receptor mutants with deletions of beta-chain variable region genes.

Ganglioside GM3: an acidic membrane component that increases during macrophage-like cell differentiation can induce monocytic differentiation of human myeloid and monocytoid leukemic cell lines HL-60 and U937.

Development and regional expression of beta nerve growth factor messenger RNA and protein in the rat central nervous system.

Study of an octadecaneuropeptide derived from diazepam binding inhibitor (DBI): biological activity and presence in rat brain.

High-affinity binding of granulocyte-macrophage colony-stimulating factor to normal and leukemic human myeloid cells.

Secondary, tertiary, and quaternary structure of T-cell-specific immunoglobulin-like polypeptide chains.

Lineage-related polypeptide markers in acute lymphoblastic leukemia detected by two-dimensional gel electrophoresis.

Evolution and organization of the human protein C gene.

Short synthetic oligodeoxyribonucleotide leader sequences enhance accumulation of human proinsulin synthesized in Escherichia coli.

Purification of microsomal signal peptidase as a complex.

Plasma high density lipoprotein is increased in man when low density lipoprotein (LDL) is lowered by LDL-pheresis.

Insulin-like growth factor I/somatomedin C: a potent inducer of oligodendrocyte development.

Year: 1986 Volume: 83 Issue: 2

Year: 1986 Volume: 83 Issue: 1

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Capacity of RecA protein to bind preferentially to UV lesions and inhibit the editing subunit (epsilon) of DNA polymerase III: a possible mechanism for SOS-induced targeted mutagenesis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Scheuermann, R. H. Lu, C. Echols, H.
Abstract	The RecA protein of Escherichia coli is required for SOS-induced mutagenesis in addition to its recombinational and regulatory roles. Most SOS-induced mutations probably occur during replication across a DNA lesion (targeted mutagenesis). We have suggested previously that RecA might participate in targeted mutagenesis by binding preferentially to the site of the DNA damage (e.g., pyrimidine dimer) because of its partially unwound character; DNA polymerase III (polIII) will then encounter RecA-coated DNA at the lesion and might replicate across the damaged site with reduced fidelity. In this report, we analyze at a biochemical level two major predictions of this model. With respect to lesion recognition, we show that purified RecA protein binds more efficiently to UV-irradiated double-stranded DNA than to nonirradiated DNA, as judged by filter-binding and gel electrophoresis assays. With respect to replication fidelity, Fersht and Knill-Jones [Fersht, A. R. & Knill-Jones, J. W. (1983) J. Mol. Biol. 165, 669-682] have found that RecA inhibits the 3'----5' exonuclease (editing function) of polIII holoenzyme. We extend this observation by demonstrating that RecA inhibits the exonuclease of the purified editing subunit of polIII, epsilon protein. Thus, we suggest that the activities of RecA required for targeted mutagenesis are lesion-recognition, followed by localized inhibition of the editing capacity of the epsilon subunit of polIII holoenzme. In this proposed mechanism, one activation signal for RecA for mutagenesis is the lesion itself. Because UV-irradiated, double-stranded DNA efficiently activates RecA for cleavage of the LexA repressor, the lesion itself may also often serve as an activation signal for induction of SOS-controlled genes.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	3
Volume Number	83
Language	English
Publisher	National Academy of Sciences
Publisher Date	1986-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	DNA Polymerase III Antagonists & Inhibitors Nucleic Acid Synthesis Inhibitors Rec A Recombinases Metabolism Antibody Diversity DNA Replication DNA, Bacterial Radiation Effects Exodeoxyribonucleases Genetic Variation Mutation Ultraviolet Rays Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in