NDLI: Nuclease activity of 1,10-phenanthroline-copper: sequence-specific targeting.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1986 Volume: 83 Issue: 24

Year: 1986 Volume: 83 Issue: 23

Year: 1986 Volume: 83 Issue: 22

Year: 1986 Volume: 83 Issue: 21

Year: 1986 Volume: 83 Issue: 20

Year: 1986 Volume: 83 Issue: 19

Altered levels of laminin receptor mRNA in various human carcinoma cells that have different abilities to bind laminin.

A low-molecular-weight RNA from mouse ascites cells that hybridizes to both 18S rRNA and mRNA sequences.

Basic fibroblast growth factor induces angiogenesis in vitro.

A temperature-dependent pBR322 copy number mutant resulting from a Tn5 position effect.

Identification and chemical synthesis of a ribosomal protein antigenic determinant in systemic lupus erythematosus.

Identification of two calcium channel receptor sites for [3H]nitrendipine in mammalian cardiac and smooth muscle membrane.

Survival, organization, and function of microcarrier-attached hepatocytes transplanted in rats.

Messenger RNA coding for only the alpha subunit of the rat brain Na channel is sufficient for expression of functional channels in Xenopus oocytes.

Neuroleptics of the diphenylbutylpiperidine series are potent calcium channel inhibitors.

Recombination within the myelin basic protein gene created the dysmyelinating shiverer mouse mutation.

Strategies for studying heterogeneous genetic traits in humans by using a linkage map of restriction fragment length polymorphisms.

Genetic linkage between the antigenic group (Ag) variation and the apolipoprotein B gene: assignment of the Ag locus.

Molecular detection of deletions involving band q14 of chromosome 13 in retinoblastomas.

Polymorphisms in the human haptoglobin gene cluster: chromosomes with multiple haptoglobin-related (Hpr) genes.

Tyrosine hydroxylase is expressed by neocortical neurons after transplantation.

Somatostatin selectively enhances acetylcholine-induced excitations in rat hippocampus and cortex.

An essential function for acyl carrier protein in the biosynthesis of membrane-derived oligosaccharides of Escherichia coli.

The in vivo mutagenic frequency and specificity of O6-methylguanine in phi X174 replicative form DNA.

Induced differentiation of a transformed clone of Ly-1+ B cells by clonal T cells and antigen.

Nuclease activity of 1,10-phenanthroline-copper: sequence-specific targeting.

ATP-independent type II topoisomerase from trypanosomes.

Expression of the complete human T-cell leukemia virus type I pX coding sequence as a functional protein in Escherichia coli.

Human melanoma cell lines of primary and metastatic origin express the genes encoding the chains of platelet-derived growth factor (PDGF) and produce a PDGF-like growth factor.

Phospholipase A2 and phospholipase C are activated by distinct GTP-binding proteins in response to alpha 1-adrenergic stimulation in FRTL5 thyroid cells.

Cloning, characterization, expression, and chromosomal localization of a human ferritin heavy-chain gene.

Identification and purification of a bacterial ice-nucleation protein.

Production of platelet-derived growth factor-like molecules by cultured arterial smooth muscle cells accompanies proliferation after arterial injury.

Multiple chromosomal rearrangements in a spontaneously arising t(6;7) rat immunocytoma juxtapose c-myc and immunoglobulin heavy chain sequences.

Expression of T-cell-activating protein in peripheral lymphocyte subsets.

Vasoactive intestinal peptide induces the synthesis of the cholesterol side-chain cleavage enzyme complex in cultured rat ovarian granulosa cells.

Identification and properties of N-methyl-D-aspartate receptors in rat brain synaptic plasma membranes.

Cloning and expression of cDNA for human diazepam binding inhibitor, a natural ligand of an allosteric regulatory site of the gamma-aminobutyric acid type A receptor.

DNA sequence adjacent to and specific for the 1.672 g/cm3 satellite DNA in the Drosophila genome.

Islet-activating protein inhibits leukotriene D4- and leukotriene C4- but not bradykinin- or calcium ionophore-induced prostacyclin synthesis in bovine endothelial cells.

Dispersal of the parasitic ciliate Lambornella clarki: implications for ciliates in the biological control of mosquitoes.

Assignment of the human and mouse prion protein genes to homologous chromosomes.

AKR murine thymic leukemias are from a distinct thymic cell lineage and do not express the beta chain of the T-cell antigen receptor.

Presence of preactivated T cells in hemodialyzed patients: their possible role in altered immunity.

Monoclonal antibody specific for an activated RAS protein.

Thermal-mechanical fluctuations enhance repulsion between bimolecular layers.

Common and distinct tubulin binding sites for microtubule-associated proteins.

Genomic sequencing reveals a positive correlation between the kinetics of strand-specific DNA demethylation of the overlapping estradiol/glucocorticoid-receptor binding sites and the rate of avian vitellogenin mRNA synthesis.

Isolation and subunit composition of tuftsin receptor.

Cloning and sequence analysis of cDNA for human argininosuccinate lyase.

Putative diazepam binding inhibitor peptide: cDNA clones from rat.

Chloroplasts of higher plants synthesize L-phenylalanine via L-arogenate.

Distinct transcription factors bind specifically to two regions of the human histone H4 promoter.

Crystal structure of a prokaryotic ribosomal protein.

Monte Carlo simulations on an equilibrium globular protein folding model.

Color discrimination in halobacteria: spectroscopic characterization of a second sensory receptor covering the blue-green region of the spectrum.

Molecular characterization and phylogenetic studies of a wound-inducible proteinase inhibitor I gene in Lycopersicon species.

Nucleotide sequence of rat preputial gland beta-glucuronidase cDNA and in vitro insertion of its encoded polypeptide into microsomal membranes.

Mechanosensitivity of cultured ciliated cells from the mammalian respiratory tract: implications for the regulation of mucociliary transport.

Regulation of melanoma by the embryonic skin.

Translocation of protein kinase C in human leukemia cells susceptible or resistant to differentiation induced by phorbol 12-myristate 13-acetate.

Long-term expression of isomyosins and myoendocrine functions in ectopic grafts of atrial tissue.

Retinol-binding protein and transthyretin mRNA levels in visceral yolk sac and liver during fetal development in the rat.

Gene-culture coevolution of complex social behavior: human altruism and mate choice.

Two mechanisms for directional gene conversion.

Localization of a lymphocyte-specific protein tyrosine kinase gene (lck) at a site of frequent chromosomal abnormalities in human lymphomas.

Suppression of type II collagen-induced arthritis by intragastric administration of soluble type II collagen.

Glucocerebrosidase processing in normal fibroblasts and in fibroblasts from patients with type I, type II, and type III Gaucher disease.

Ocular delayed hypersensitivity: a pathogenetic mechanism of chlamydial-conjunctivitis in guinea pigs.

Epoxidation of (+/-)-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene during (bi)sulfite autoxidation: activation of a procarcinogen by a cocarcinogen.

From basic network principles to neural architecture: emergence of spatial-opponent cells.

Analgesia induced by isolated bovine chromaffin cells implanted in rat spinal cord.

Sexual dimorphism in human and canine spinal cord: role of early androgen.

Basic fibroblast growth factor supports the survival of cerebral cortical neurons in primary culture.

Inhibition of the in vivo parathyroid hormone-mediated calcemic response in rats by a synthetic hormone antagonist.

Developmental aspects of proline transport in rat renal brush border membranes.

Myristylation site in Pr65gag is essential for virus particle formation by Moloney murine leukemia virus.

Stable integration of mouse DNA into Ia-negative human B-lymphoma cells causes reexpression of the human Ia-positive phenotype.

Isolation and characterization of a primitive interleukin-1-like protein from an invertebrate, Asterias forbesi.

Tumor necrosis factor type alpha stimulates human endothelial cells to produce granulocyte/macrophage colony-stimulating factor.

High-level expression of enzymatically active human Cu/Zn superoxide dismutase in Escherichia coli.

Restoration of flagellar clockwise rotation in bacterial envelopes by insertion of the chemotaxis protein CheY.

Oligonucleotide-directed double-strand break repair in plasmids of Escherichia coli: a method for site-specific mutagenesis.

Isolation and characterization of a cDNA encoding a human liver/bone/kidney-type alkaline phosphatase.

Isolation of an episomal yeast gene and replication origin as chromatin.

Structural evidence that endothelial cell growth factor beta is the precursor of both endothelial cell growth factor alpha and acidic fibroblast growth factor.

A cell surface desmosome-associated component: identification of tissue-specific cell adhesion molecule.

Intranuclear appearance of the phosphorylated form of cytoskeleton-associated 350-kDa proteins in U1-ribonucleoprotein regions after growth stimulation of fibroblasts.

Transformation of Phycomyces blakesleeanus to G-418 resistance by an autonomously replicating plasmid.

Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases.

Promoters selected from random DNA sequences.

Ia+ murine epidermal Langerhans cells are deficient in surface expression of the class I major histocompatibility complex.

Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db.

Expression of the F glycoprotein of respiratory syncytial virus by a recombinant vaccinia virus: comparison of the individual contributions of the F and G glycoproteins to host immunity.

Analysis by immunocytochemistry and in situ hybridization of renin and its mRNA in kidney, testis, adrenal, and pituitary of the rat.

Year: 1986 Volume: 83 Issue: 18

Year: 1986 Volume: 83 Issue: 17

Year: 1986 Volume: 83 Issue: 16

Year: 1986 Volume: 83 Issue: 15

Year: 1986 Volume: 83 Issue: 14

Year: 1986 Volume: 83 Issue: 13

Year: 1986 Volume: 83 Issue: 12

Year: 1986 Volume: 83 Issue: 11

Year: 1986 Volume: 83 Issue: 10

Year: 1986 Volume: 83 Issue: 9

Year: 1986 Volume: 83 Issue: 8

Year: 1986 Volume: 83 Issue: 7

Year: 1986 Volume: 83 Issue: 6

Year: 1986 Volume: 83 Issue: 5

Year: 1986 Volume: 83 Issue: 4

Year: 1986 Volume: 83 Issue: 3

Year: 1986 Volume: 83 Issue: 2

Year: 1986 Volume: 83 Issue: 1

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Nuclease activity of 1,10-phenanthroline-copper: sequence-specific targeting.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chen, C. H. Sigman, D. S.
Abstract	The nuclease activity of 1,10-phenanthroline-copper ion can be targeted to specific DNA sequences by attachment of the ligand to the 5' end of complementary deoxyoligonucleotides via a phosphoramidate linkage. To synthesize the adduct, the phosphorimidazolide of the deoxyoligonucleotide is prepared using a water-soluble carbodiimide and is then coupled to 5-glycylamido-1,10-phenanthroline. After hybridization to the target DNA, sequence-specific cleavage is observed upon the addition of cupric ion and 3-mercaptopropionic acid. Two methods of assaying the cutting of the operator sequence of the lac operon have been employed using the oligonucleotide 5'-AATTGTTATCCGCTCACAATT-3' representing sequence positions 21-1 of the template strand. In the first, the single-stranded DNA of the phage M13mp8 was the target, and cuts were detected using a primer-extension assay. In the second, the substrate was an EcoRI fragment 3' labeled in the nontemplate strand. After denaturation and reannealing to the oligonucleotide-1,10-phenanthroline adduct, cupric ion and 3-mercaptopropionic acid were added, and the products were analyzed directly on a sequencing gel. With the phenanthroline moiety attached to position 21 of the oligonucleotide carrier, cutting was observed at positions 20-25 using both assays.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	19
Volume Number	83
Language	English
Publisher	National Academy of Sciences
Publisher Date	1986-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Deoxyribonucleases Phenanthrolines Catalysis DNA Restriction Enzymes Hydrogen Peroxide Lac Operon Oligonucleotides Substrate Specificity Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in