NDLI: Polyadenylylation of an mRNA precursor occurs independently of transcription by RNA polymerase II in vivo.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1986 Volume: 83 Issue: 24

Year: 1986 Volume: 83 Issue: 23

Year: 1986 Volume: 83 Issue: 22

Vasopressin-mediated inhibition of atrial natriuretic factor-stimulated cGMP accumulation in an established smooth muscle cell line.

Barium modulates c-fos expression and post-translational modification.

Purification and characterization of the amiloride-sensitive sodium channel from A6 cultured cells and bovine renal papilla.

Properties of a mouse ribosomal protein promoter.

Functional analysis of regulatory elements in a plant embryo-specific gene.

cDNA and amino acid sequences of the cell adhesion protein receptor recognizing vitronectin reveal a transmembrane domain and homologies with other adhesion protein receptors.

Stable repression of ribosomal protein L1 synthesis in Xenopus oocytes by microinjection of antisense RNA.

Immunization of mice with purified U1 small nuclear ribonucleoprotein (RNP) induces a pattern of antibody specificities characteristic of the anti-Sm and anti-RNP autoimmune response of patients with lupus erythematosus, as measured by monoclonal antibodies.

In vitro modification of human immunodeficiency virus infection by granulocyte-macrophage colony-stimulating factor and gamma interferon.

Structure and replication of the genome of the hepatitis delta virus.

Additional component in the cellular mechanism of presynaptic facilitation contributes to behavioral dishabituation in Aplysia.

Charge movement and depolarization-contraction coupling in arthropod vs. vertebrate skeletal muscle.

Brain voltage-sensitive calcium channel subtypes differentiated by omega-conotoxin fraction GVIA.

Monoclonal antibodies to glutamic acid decarboxylase.

Polyadenylylation of an mRNA precursor occurs independently of transcription by RNA polymerase II in vivo.

Serotonin initiates and autoamplifies its own synthesis during mouse central nervous system development.

Antibody-targeted photolysis: selective photodestruction of human T-cell leukemia cells using monoclonal antibody-chlorin e6 conjugates.

1,3-Di(2-[5-3H]tolyl)guanidine: a selective ligand that labels sigma-type receptors for psychotomimetic opiates and antipsychotic drugs.

Transplantation of ventral mesencephalic anlagen to hosts with genetic nigrostriatal dopamine deficiency.

Bovine brain kinesin is a microtubule-activated ATPase.

Sequence and expression of transcripts of the T-cell antigen receptor alpha-chain gene in a functional, antigen-specific suppressor-T-cell hybridoma.

Anti-beta-endorphin immunoglobulin G in humans.

Combination of a long-acting delivery system for luteinizing hormone-releasing hormone agonist with Novantrone chemotherapy: increased efficacy in the rat prostate cancer model.

Altered effector specificities in regulators of gene expression: TOL plasmid xylS mutants and their use to engineer expansion of the range of aromatics degraded by bacteria.

Mouse thymidylate synthase messenger RNA lacks a 3' untranslated region.

Phosphatidylinositol 4,5-bisphosphate turnover is transient while phosphatidylinositol turnover is persistent in thyrotropin-releasing hormone-stimulated rat pituitary cells.

Interaction of a nuclear factor with the polyomavirus enhancer region.

Differential sensitivity of two functions of the insulin receptor to the associated proteolysis: kinase action and hormone binding.

Structural homology of reaction centers from Rhodopseudomonas sphaeroides and Rhodopseudomonas viridis as determined by x-ray diffraction.

cAMP induction of prespore and prestalk gene expression in Dictyostelium is mediated by the cell-surface cAMP receptor.

Suppression of tumor growth by senescence in virally transformed human fibroblasts.

Charomids: cosmid vectors for efficient cloning and mapping of large or small restriction fragments.

Molecular structure of a somatically unstable transposable element in Drosophila.

Transformation of human ciliary epithelial cells by simian virus 40: induction of cell proliferation and retention of beta 2-adrenergic receptors.

A human cytomegalovirus mutant resistant to the nucleoside analog 9-([2-hydroxy-1-(hydroxymethyl)ethoxy]methyl)guanine (BW B759U) induces reduced levels of BW B759U triphosphate.

Structure, evolution, and polymorphisms of the human apolipoprotein A4 gene (APOA4).

Tissue-specific and constitutive alpha-tubulin genes of Drosophila melanogaster code for structurally distinct proteins.

Cell-type-specific transcription of an immunoglobulin kappa light chain gene in vitro.

Photocrosslinking of the signal sequence of nascent preprolactin to the 54-kilodalton polypeptide of the signal recognition particle.

Genes for synapsin I, a neuronal phosphoprotein, map to conserved regions of human and murine X chromosomes.

Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase.

A repetitive antigen of Plasmodium falciparum that is homologous to heat shock protein 70 of Drosophila melanogaster.

The role of the T-cell receptor in thymocyte maturation: effects in vivo of anti-receptor antibody.

Evidence for methoxatin (pyrroloquinolinequinone) as the cofactor in bovine plasma amine oxidase from resonance Raman spectroscopy.

'Footprint' titrations yield valid thermodynamic isotherms.

Binding of ethidium bromide causes dissociation of the nucleosome core particle.

Molecular models for the putative dimer of sea lamprey hemoglobin.

Molecular cloning of mouse placental lactogen cDNA.

Mutagenic potential of O4-methylthymine in vivo determined by an enzymatic approach to site-specific mutagenesis.

GCN4 protein, a positive transcription factor in yeast, binds general control promoters at all 5' TGACTC 3' sequences.

Isozymic forms of rat brain Ca2+-activated and phospholipid-dependent protein kinase.

U6 small nuclear RNA is transcribed by RNA polymerase III.

Two-dimensional crystals of cholera toxin B-subunit-receptor complexes: projected structure at 17-A resolution.

Direct evidence for the existence of a neutrophil-derived platelet activator (neutrophilin).

Evidence for a circaseptan and a circasemiseptan growth response to light/dark cycle shifts in nucleated and enucleated Acetabularia cells, respectively.

Sequence of mRNA coding for bindin, a species-specific sea urchin sperm protein required for fertilization.

Characterization of the boundaries between adjacent rapidly and slowly evolving genomic regions in Drosophila.

An intervening sequence in an unusual histone H1 gene of Tetrahymena thermophila.

Regression of cutaneous metastatic melanoma by intralesional injection with human monoclonal antibody to ganglioside GD2.

Genes for the tumor necrosis factors alpha and beta are linked to the human major histocompatibility complex.

Expression of major histocompatibility complex class I antigens as a strategy for the potentiation of immune recognition of tumor cells.

Immunoglobulin and T-cell receptor gene rearrangement and expression in human lymphoid leukemia cells at different stages of maturation.

From basic network principles to neural architecture: emergence of orientation columns.

Gene 32 protein, the single-stranded DNA binding protein from bacteriophage T4, is a zinc metalloprotein.

Identification of two anti-parallel beta-sheet conformations in the solution structure of murine epidermal growth factor by proton magnetic resonance.

Regulation of expression of human granulocyte/macrophage colony-stimulating factor.

Molecular cloning of the human T-lymphocyte surface CD2 (T11) antigen.

Interleukin 1 induces leukocyte infiltration and cartilage proteoglycan degradation in the synovial joint.

beta-Glucuronidase from Escherichia coli as a gene-fusion marker.

Translation of a synthetic two-cistron mRNA in Escherichia coli.

Identification of the parasite transferrin receptor of Plasmodium falciparum-infected erythrocytes and its acylation via 1,2-diacyl-sn-glycerol.

Year: 1986 Volume: 83 Issue: 21

Year: 1986 Volume: 83 Issue: 20

Year: 1986 Volume: 83 Issue: 19

Year: 1986 Volume: 83 Issue: 18

Year: 1986 Volume: 83 Issue: 17

Year: 1986 Volume: 83 Issue: 16

Year: 1986 Volume: 83 Issue: 15

Year: 1986 Volume: 83 Issue: 14

Year: 1986 Volume: 83 Issue: 13

Year: 1986 Volume: 83 Issue: 12

Year: 1986 Volume: 83 Issue: 11

Year: 1986 Volume: 83 Issue: 10

Year: 1986 Volume: 83 Issue: 9

Year: 1986 Volume: 83 Issue: 8

Year: 1986 Volume: 83 Issue: 7

Year: 1986 Volume: 83 Issue: 6

Year: 1986 Volume: 83 Issue: 5

Year: 1986 Volume: 83 Issue: 4

Year: 1986 Volume: 83 Issue: 3

Year: 1986 Volume: 83 Issue: 2

Year: 1986 Volume: 83 Issue: 1

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Polyadenylylation of an mRNA precursor occurs independently of transcription by RNA polymerase II in vivo.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Manley, J. L. Lewis, E. D.
Abstract	Most eukaryotic messenger RNAs are transcribed as precursor molecules that must be processed by capping, splicing, 3' cleavage, and polyadenylylation to yield mature mRNAs. An important, unresolved issue is whether any of these reactions are linked either to transcription by RNA polymerase II or to each other. To address one aspect of this question, we constructed a chimeric gene containing an RNA polymerase III promoter (the adenovirus VAI promoter) fused to the body and 3'-flanking sequences of a protein-coding gene (the herpesvirus tk gene). Here we show that this hybrid gene was transcribed from the RNA polymerase III promoter following transfection of human 293 cells and that the transcripts produced were stable and efficiently transported to the cytoplasm. Although a significant proportion of the transcripts were prematurely terminated at specific sites within the gene, a high percentage of the full-length RNA was accurately cleaved and polyadenylylated. These results demonstrate that cleavage and polyadenylylation of mRNA precursors are not obligatorily coupled to transcription by RNA polymerase II in vivo.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	22
Volume Number	83
Language	English
Publisher	National Academy of Sciences
Publisher Date	1986-12-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Nucleic Acid Precursors Metabolism Poly A RNA Polymerase II Pharmacology RNA, Messenger Transcription, Genetic Endonucleases Nucleotide Mapping Promoter Regions, Genetic RNA Polymerase III RNA Precursors Single-Strand Specific DNA And RNA Endonucleases Thymidine Kinase Genetics Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in