NDLI: Cloning and expression of cDNA for human poly(ADP-ribose) polymerase.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1987 Volume: 84 Issue: 24

Year: 1987 Volume: 84 Issue: 23

Year: 1987 Volume: 84 Issue: 22

Year: 1987 Volume: 84 Issue: 21

Year: 1987 Volume: 84 Issue: 20

Year: 1987 Volume: 84 Issue: 19

Year: 1987 Volume: 84 Issue: 18

Year: 1987 Volume: 84 Issue: 17

Year: 1987 Volume: 84 Issue: 16

Year: 1987 Volume: 84 Issue: 15

Year: 1987 Volume: 84 Issue: 14

Year: 1987 Volume: 84 Issue: 13

Year: 1987 Volume: 84 Issue: 12

Year: 1987 Volume: 84 Issue: 11

Year: 1987 Volume: 84 Issue: 10

Year: 1987 Volume: 84 Issue: 9

Year: 1987 Volume: 84 Issue: 8

Year: 1987 Volume: 84 Issue: 7

Year: 1987 Volume: 84 Issue: 6

Year: 1987 Volume: 84 Issue: 5

Partly native epitopes are already present on early intermediates in the folding of tryptophan synthase.

Site-directed mutagenesis of the COOH-terminal region of colicin A: effect on secretion and voltage-dependent channel activity.

Abundant expression of homeobox genes in mouse embryonal carcinoma cells correlates with chemically induced differentiation.

Isolation of temperature-sensitive tyrosine kinase mutants of v-abl oncogene by screening with antibodies for phosphotyrosine.

Evolutionary relationship between the natural anti-Gal antibody and the Gal alpha 1----3Gal epitope in primates.

Synthetic gene construct expressing a repeated and highly immunogenic epitope of the Plasmodium falciparum antigen Pf155.

Mutational analysis of the human immunodeficiency virus: the orf-B region down-regulates virus replication.

The Thy-1 antigen exhibits rapid lateral diffusion in the plasma membrane of rodent lymphoid cells and fibroblasts.

Alpha 2-adrenergic receptor-mediated sensitization of forskolin-stimulated cyclic AMP production.

The yeast PHO5 promoter: phosphate-control elements and sequences mediating mRNA start-site selection.

Immunochemical studies of fibroblasts from patients with methylmalonyl-CoA mutase apoenzyme deficiency: detection of a mutation interfering with mitochondrial import.

Tunicamycin-resistant Leishmania mexicana amazonensis: expression of virulence associated with an increased activity of N-acetylglucosaminyltransferase and amplification of its presumptive gene.

'E3/19K' protein of adenovirus type 2 inhibits lysis of cytolytic T lymphocytes by blocking cell-surface expression of histocompatibility class I antigens.

Identification of a monoclonal antibody specific for a murine T3 polypeptide.

Fusion as a mediator of cytolysis in mixtures of uninfected CD4+ lymphocytes and cells infected by human immunodeficiency virus.

Developmental acquisition of DNase I sensitivity of the phosphoenolpyruvate carboxykinase (GTP) gene in rat liver.

Chromatin structure is required to block transcription of the methylated herpes simplex virus thymidine kinase gene.

A promoterless retroviral vector indicates that there are sequences in U3 required for 3' RNA processing.

Regulation of c-myc and c-fos mRNA levels by polyomavirus: distinct roles for the capsid protein VP1 and the viral early proteins.

The E2 'gene' of bovine papillomavirus encodes an enhancer-binding protein.

Cloning and expression of cDNA for human poly(ADP-ribose) polymerase.

Proton NMR measurements of bacteriophage T4 lysozyme aided by 15N isotopic labeling: structural and dynamic studies of larger proteins.

Yeast cytochrome c with phenylalanine or tyrosine at position 87 transfers electrons to (zinc cytochrome c peroxidase)+ at a rate ten thousand times that of the serine-87 or glycine-87 variants.

Purification of the colony-stimulating factor 1 receptor and demonstration of its tyrosine kinase activity.

Inducible binding of a factor to the c-fos regulatory region.

Cell-type-specific responses of RT4 neural cell lines to dibutyryl-cAMP: branch determination versus maturation.

Transfection of a rearranged viral DNA fragment, WZhet, stably converts latent Epstein-Barr viral infection to productive infection in lymphoid cells.

Direct measurement of free radical generation following reperfusion of ischemic myocardium.

Basal and adenosine receptor-stimulated levels of cAMP are reduced in lymphocytes from alcoholic patients.

Protracted treatment with diazepam increases the turnover of putative endogenous ligands for the benzodiazepine/beta-carboline recognition site.

Aldosterone activates Na+/H+ exchange and raises cytoplasmic pH in target cells of the amphibian kidney.

Hydrocarbon formation in the reductive cleavage of hydroperoxides by cytochrome P-450.

Poly(ADP-ribose) may signal changing metabolic conditions to the chromatin of mammalian cells.

Antigen-receptor interaction requirement for conjugate formation and lethal-hit triggering by cytotoxic T lymphocytes can be bypassed by protein kinase C activators and Ca2+ ionophores.

Crosslinking of surface antigens causes mobilization of intracellular ionized calcium in T lymphocytes.

Pharmacokinetics of aspirin and salicylate in relation to inhibition of arachidonate cyclooxygenase and antiinflammatory activity.

Fusion between endocytic vesicles in a cell-free system.

Promoter mapping and cell cycle regulation of flagellin gene transcription in Caulobacter crescentus.

Amino acid sequence of rabbit fast-twitch skeletal muscle calsequestrin deduced from cDNA and peptide sequencing.

Expression of a set of growth-related immediate early genes in BALB/c 3T3 cells: coordinate regulation with c-fos or c-myc.

Hepatitis B surface antigen gene expression is regulated by sex steroids and glucocorticoids in transgenic mice.

A method for the rapid assignment of 1H NMR spectra of oligosaccharides using homonuclear Hartmann-Hahn spectroscopy.

Synthesis of inositol 1,2-(cyclic)-4,5-trisphosphate.

3-Phosphoglycerate-dependent protein phosphorylation.

Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: close homology to placental alkaline phosphatase.

Phase transitions in sequence matches and nucleic acid structure.

Transformation of normal rat kidney (NRK) cells by an infectious retrovirus carrying a synthetic rat type alpha transforming growth factor gene.

Alteration of the tumorigenic and metastatic properties of neoplastic cells is associated with the process of calcium phosphate-mediated DNA transfection.

Modulation of cytosolic free calcium levels by extracellular phosphate and lanthanum.

Ha-ras oncogene expression directed by a milk protein gene promoter: tissue specificity, hormonal regulation, and tumor induction in transgenic mice.

Bizarre tRNAs inferred from DNA sequences of mitochondrial genomes of nematode worms.

Altered cell-surface protein(s), crossreactive with DNA, on spleen cells of autoimmune lupic mice.

Modification of erythrocyte enzyme activities by persulfides and methanethiol: possible regulatory role.

Anatomical relationship between the basal ganglia and the basal nucleus of Meynert in human and monkey forebrain.

Peroxisomal beta-oxidation enzyme proteins in adrenoleukodystrophy: distinction between X-linked adrenoleukodystrophy and neonatal adrenoleukodystrophy.

Depolarization-induced Ca2+ increase in isolated neurosecretory nerve terminals measured with fura-2.

Isolation of enzyme cDNA clones by enzyme immunodetection assay: isolation of a peptide acetyltransferase.

Aberrant splicing of proteolipid protein mRNA in the dysmyelinating jimpy mutant mouse.

Synthesis of sodium channels in the cell bodies of squid giant axons.

Cloning and tissue-specific expression of mouse macrophage colony-stimulating factor mRNA.

Ribonucleotide-induced helical alteration in DNA prevents nucleosome formation.

Altered protein kinase C in a mast cell variant defective in exocytosis.

Isolation of high-affinity memory B cells: phycoerythrin as a probe for antigen-binding cells.

Single cell studies on the role of B-cell stimulatory factor 1 in B-cell activation.

Cloning, sequence, and expression of the lysostaphin gene from Staphylococcus simulans.

Prokaryotic and eukaryotic RNA polymerases have homologous core subunits.

Designing substrate specificity by protein engineering of electrostatic interactions.

DNA rearrangements in human follicular lymphoma can involve the 5' or the 3' region of the bcl-2 gene.

Insulin binding to human B lymphoblasts is a function of HLA haplotype.

Year: 1987 Volume: 84 Issue: 4

Year: 1987 Volume: 84 Issue: 3

Year: 1987 Volume: 84 Issue: 2

Year: 1987 Volume: 84 Issue: 1

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Cloning and expression of cDNA for human poly(ADP-ribose) polymerase.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Alkhatib, H. M. Bhatia, K. Notario, V. Stein, G. Smulson, M. E. Cherney, B. Roeder, R. G. Slattery, E. Chen, D. F. Giri, C.
Abstract	cDNAs encoding poly(ADP-ribose) polymerase from a human hepatoma lambda gt11 cDNA library were isolated by immunological screening. One insert of 1.3 kilobases (kb) consistently hybridized on RNA gel blots to an mRNA species of 3.6-3.7 kb, which is consistent with the size of RNA necessary to code for the polymerase protein (116 kDa). This insert was subsequently used in both in vitro hybrid selection and hybrid-arrested translation studies. An mRNA species from HeLa cells of 3.6-3.7 kb was selected that was translated into a 116-kDa protein, which was selectively immunoprecipitated with anti-poly (ADP-ribose) polymerase. To confirm that the 1.3-kb insert from lambda gt11 encodes for poly(ADP-ribose) polymerase, the insert was used to screen a 3- to 4-kb subset of a transformed human fibroblast cDNA library in the Okayama-Berg vector. One of these vectors [pcD-p(ADPR)P; 3.6 kb] was tested in transient transfection experiments in COS cells. This cDNA insert contained the complete coding sequence for polymerase as indicated by the following criteria: A 3-fold increase in in vitro activity was noted in extracts from transfected cells compared to mock or pSV2-CAT transfected cells. A 6-fold increase in polymerase activity in pcD-p(ADPR)P transfected cell extracts compared to controls was observed by 'activity gel' analysis on gels of electrophoretically separated proteins at 116 kDa. A 10- to 15-fold increase in newly synthesized polymerase was detected by immunoprecipitation of labeled transfected cell extracts. Using pcD-p(ADPR)P as probe, it was observed that the level of poly(ADP-ribose) polymerase mRNA was elevated at 5 and 7 hr of S phase of the HeLa cell cycle, but was unaltered when artificial DNA strand breaks are introduced in HeLa cells by alkylating agents.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	5
Volume Number	84
Language	English
Publisher	National Academy of Sciences
Publisher Date	1987-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cloning, Molecular Gene Expression Regulation Genes Poly(ADP-ribose) Polymerases Genetics Carcinoma, Hepatocellular Enzymology Liver Neoplasms Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger Isolation & Purification Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in