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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Velleman, M. Schuster, H. Dreiseikelmann, B. |
| Abstract | After digestion of bacteriophage P1 DNA with EcoRI in the presence of P1 repressor, 6 repressor binding sites were identified in 5 of 26 EcoRI fragments. Binding sites were localized by the decreased mobility of DNA fragment-repressor complexes during electrophoresis and by DNase protection ("footprinting") analysis. The repressor binding sites, or operators, comprise a 17-base-pair-long consensus sequence lacking symmetrical elements. Three operators can be related to known genes, whereas the function of the others is still unknown. The mutant P1 bac, rendering ban expression constitutive, is identified as an operator-constitutive mutation of the ban operon. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 16 |
| Volume Number | 84 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1987-09-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Bacteriophages Genetics DNA, Viral Metabolism Repressor Proteins Transcription Factors Binding Sites Cloning, Molecular DNA Restriction Enzymes Deoxyribonuclease BamHI Deoxyribonuclease EcoRI Deoxyribonucleases Mutation Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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