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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Reisine, T. He, H. T. Johnson, K. Thermos, K. |
| Description | Author Affiliation: He HT ( Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104.); |
| Abstract | The brain somatostatin (somatotropin release-inhibiting factor; SRIF) receptor was purified by affinity chromatographic techniques. A protein of 60 kDa could be purified from rat brain. The protein was eluted from a [D-Trp8]SRIF affinity column with either sodium acetate (pH 5.5) or free [D-Trp8]SRIF. The binding of the protein to the affinity column was prevented by free [D-Trp8]SRIF or the stable SRIF analogue SMS 201-996 but not by the inactive somatostatin 28-(1-14). The purified receptor could be covalently labeled by the 125I-labeled SRIF analogue CGP 23996. Excess [D-Trp8]SRIF blocked the binding of 125I-labeled CGP 23996 to the purified receptor, but somatostatin 28-(1-14) did not affect the binding. A 60-kDa protein was also purified from the anterior pituitary cell line AtT-20, which has a high expression of SRIF receptors. In contrast, no 60-kDa protein could be purified from CHO cells, which have no detectable SRIF receptors. These findings present evidence for the purification of the SRIF receptor. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 5 |
| Volume Number | 86 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1989-04-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Brain Metabolism Receptors, Neurotransmitter Isolation & Purification Affinity Labels Animals Cell Line Cell Membrane Chromatography, Affinity Kinetics Molecular Weight Rats, Inbred Strains Receptors, Somatostatin Somatostatin Analogs & Derivatives Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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