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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Popoff, S. C. Spira, A. I. Johnson, A. W. Demple, B. |
| Description | Author Affiliation: Popoff SC ( Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.); |
| Abstract | DNA damage generated by oxygen radicals includes base-free apurinic/apyrimidinic (AP) sites and strand breaks that bear deoxyribose fragments. The yeast Saccharomyces cerevisiae repairs such DNA lesions by using a single major enzyme. We have cloned the yeast structural gene (APN1) encoding this AP endonuclease/3'-repair diesterase by immunological screening of a yeast genomic DNA expression library in lambda gt11. Gene disruption experiments confirm that the Apn1 protein accounts for greater than or equal to 97% of both AP endonuclease and DNA 3'-repair diesterase activities in yeast cell-free extracts. The DNA and predicted amino acid sequences for the APN1 gene are homologous to those for the nfo gene encoding DNA endonuclease IV of Escherichia coli. This conservation of structure between a eukaryotic enzyme and its prokaryotic counterpart underscores the fundamental nature of their roles in DNA repair. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 11 |
| Volume Number | 87 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1990-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Endodeoxyribonucleases Genetics Escherichia Coli Proteins Genes, Fungal Saccharomyces Cerevisiae Amino Acid Sequence Blotting, Western Cloning, Molecular DNA Repair DNA-(Apurinic Or Apyrimidinic Site) Lyase Deoxyribonuclease IV (Phage T4-Induced) Escherichia Coli Enzymology Molecular Sequence Data Restriction Mapping Comparative Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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