NDLI: Distribution of interspersed repeats (Alu and Kpn) on NotI restriction fragments of human chromosome 21.

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Distribution of interspersed repeats (Alu and Kpn) on NotI restriction fragments of human chromosome 21.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Pevny, L. Wu, Y. Cantor, C. R. Sainz, J. Smith, C. L.
Description	Author Affiliation: Sainz J ( Division of Chemical Biodynamics, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.);
Abstract	Interspersed repeated sequences (Alu and Kpn) were used as probes to detect a set of Not I restriction fragments of human chromosome 21 from the hybrid cell line WAV17. Forty different Not I fragments, ranging in size from less than 0.05 megabase (Mb) to 7.0 Mb, were identified. The total length of these fragments was 47.3 Mb. This length provides an estimate of the minimum size of the chromosome and a minimum number of fragments to be ordered to create a complete restriction map. The average length Not I fragment is 1.2 Mb. Alu and Kpn fragments are not always coincident: a 2.9-Mb fragment is detected with Kpn but not with Alu, and 13 fragments, ranging from less than 0.05 Mb to 5.6 Mb, are detected with Alu but not with Kpn; the 26 remaining fragments, covering 75% (35.3 Mb) of the total length, are detected with both repetitive probes. The presence of so many noncoincident fragments and the high variation of the hybridization signal intensities of the fragments suggest a very nonuniform distribution of Kpn and Alu repeats.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	3
Volume Number	89
Language	English
Publisher	National Academy of Sciences
Publisher Date	1992-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Chromosomes, Human, Pair 21 Ultrastructure Repetitive Sequences, Nucleic Acid Restriction Mapping Blotting, Southern DNA Probes Deoxyribonucleases, Type II Site-Specific Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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