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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Cahir Mcfarland, E. D. Sefton, B. M. Hurley, T. R. Pingel, J. T. Shaw, A. Thomas, M. L. |
| Description | Author Affiliation: Cahir McFarland ED ( Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.); |
| Abstract | Stimulation of tyrosine phosphorylation is an early and important event in antigen-induced T-cell activation. T-cell clones deficient in expression of CD45, a transmembrane protein-tyrosine-phosphatase (protein-tyrosine-phosphate phosphohydrolase, EC 3.1.3.48), are impaired in their ability to respond to either antigen or T-cell receptor cross-linking. Analysis of the CD45-deficient CD8+ T-cell clone L3M-93 demonstrates that the Src family members p56lck and p59fyn show increased immunoreactivity with anti-phosphotyrosine antibody and exhibit decreased kinase activity. The site of increased tyrosine phosphorylation in Src family members was identified by comparison of cyanogen bromide peptide maps. Phosphorylation of the C-terminal phosphopeptide, containing the negative regulatory site of tyrosine phosphorylation, from the CD45-deficient cells was increased 8-fold for p56lck and 2-fold for p59fyn. These data suggest that CD45 dephosphorylates the negative regulatory site of multiple Src family members in the cytotoxic T-lymphocyte clone L3 and show a correlation between the ability to respond efficiently to antigen and the dephosphorylation of Src family members by CD45. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 4 |
| Volume Number | 90 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1993-03-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Antigens, CD45 Metabolism Genes, Src Protein Tyrosine Phosphatases Protein-Tyrosine Kinases Proto-Oncogene Proteins Receptors, Antigen, T-Cell Physiology Signal Transduction T-Lymphocytes Immunology Animals Cell Membrane Clone Cells Cyanogen Bromide Immunoblotting Lymphocyte Activation Lymphocyte Specific Protein Tyrosine Kinase P56(lck) Peptide Fragments Isolation & Purification Peptide Mapping Phosphorylation Proto-Oncogene Proteins C-fyn Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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