NDLI: Recombinant thyroid hormone receptor and retinoid X receptor stimulate ligand-dependent transcription in vitro.

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Recombinant thyroid hormone receptor and retinoid X receptor stimulate ligand-dependent transcription in vitro.

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Recombinant thyroid hormone receptor and retinoid X receptor stimulate ligand-dependent transcription in vitro.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Nikodem, V. M. Driggers, P. H. Lee, I. J. Ozato, K. Medin, J. A.
Description	Author Affiliation: Lee IJ ( Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.);
Abstract	The thyroid hormone and retinoid X receptors form a heterodimer with each other and mediate thyroid hormone (T3)-dependent transcription. Retinoid X receptor, in addition, forms a homodimer and mediates 9-cis-retinoic acid-dependent transcription. Here, recombinant thyroid hormone receptor and recombinant retinoid X receptor beta expressed from baculovirus vectors have been studied for ligand-mediated activation of transcription in vitro. We show that the two recombinant receptors, most likely as a heterodimer, cooperatively enhance transcription in vitro from a template containing functional T3 responsive elements. The enhancement was specific for the T3 responsive element and was greatest when T3 was added to the reaction (approximately 14-fold increase). Albeit to a lesser degree, the two receptors also directed transcription in the absence of T3. Template competition experiments suggest that the two receptors enhance formation of the preinitiation complex and that activation by T3 occurs when the ligand binds the receptor prior to (or during), but not after, the formation of the preinitiation complex. Although 9-cis-retinoic acid had no effect on the T3-dependent transcription, this ligand activated transcription in vitro directed by recombinant retinoic X receptor beta, most likely as a homodimer. This activation was observed when using nuclear extracts from embryonal carcinoma cells as a source of basal transcription factors, but not those from B lymphocytes. These results demonstrate that transcriptional activation mediated by T3 and 9-cis-retinoic acid can be reconstituted in vitro with the respective recombinant receptors.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	5
Volume Number	91
Language	English
Publisher	National Academy of Sciences
Publisher Date	1994-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Receptors, Cytoplasmic And Nuclear Genetics Receptors, Retinoic Acid Receptors, Thyroid Hormone Transcription Factors Transcriptional Activation Animals DNA In Vitro Techniques Ligands Molecular Sequence Data Metabolism Recombinant Proteins Retinoid X Receptors Tretinoin Triiodothyronine Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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