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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Brown, R. M. Lee, J. H. Shoda, S. Kuga, S. Kobayashi, S. |
| Description | Author Affiliation: Lee JH ( Department of Botany, University of Texas, Austin 78713-7640.); |
| Abstract | Cellulose microfibrils with an electron diffraction pattern characteristic of crystalline native cellulose I have been assembled abiotically by means of a cellulase-catalyzed polymerization of beta-cellobiosyl fluoride substrate monomer in acetonitrile/acetate buffer. Substantial purification of the Trichoderma viride cellulase enzyme was found to be essential for the formation of the synthetic cellulose I allomorph. Assembly of synthetic cellulose I appears to be a result of a micellar aggregation of the partially purified enzyme and the substrate in an organic/aqueous solvent system favoring the alignment of glucan chains with the same polarity and extended chain conformation, resulting in crystallization to form the metastable cellulose I allomorph. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 16 |
| Volume Number | 91 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1994-09-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cellobiose Analogs & Derivatives Cellulase Metabolism Cellulose Biosynthesis Acetates Acetonitriles Carbohydrate Sequence Isolation & Purification Cellulose 1,4-beta-Cellobiosidase Electrons Glycoside Hydrolases Gold Colloid Microscopy, Electron Molecular Sequence Data Scattering, Radiation Staining And Labeling Trichoderma Enzymology Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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