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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Tsui, S. Hwang, D. M. Fung, Y. W. Laurenssen, C. Cukerman, E. Lee, C. Y. Liew, C. C. |
| Description | Author Affiliation: Liew CC ( Department of Clinical Biochemistry, Toronto Hospital, University of Toronto, Canada.); |
| Abstract | The heart, which is composed of all the cellular components of the circulatory system, is a representative organ for obtaining genes expressed in the cardiovascular system in normal and disease states. We used partial sequences of cDNA clones, or expressed sequence tags, to identify and tag genes expressed in this organ. More than 3500 partial sequences representing > 3000 cDNA clones have been obtained from either the 5' or 3' end of inserts derived from human heart cDNA libraries. Of 3132 cDNA clones analyzed by sequence similarity searching against the GenBank/EMBL data bases, 1485 (47.4%) were found to represent additional, previously undiscovered genes, whereas 267 clones were matched to human brain expressed sequence tags. Clones matching to known genes were catalogued according to their putative structural and cellular functions. cDNA probes from reverse-transcribed mRNAs of fetal and adult hearts were used to study differential expression of selected clones in cardiac development. Cataloguing genes expressed in the heart may provide insight into the genes involved in health and cardiovascular disease. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 22 |
| Volume Number | 91 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1994-11-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cardiovascular System Metabolism Gene Expression Genes Myocardium Sequence Tagged Sites Animals Brain Physiopathology Cloning, Molecular DNA, Complementary Fetal Heart Fetus Liver RNA, Messenger Isolation & Purification Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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