NDLI: Development of a K(+)-channel probe and its use for identification of an intracellular plant membrane K+ channel.

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Development of a K(+)-channel probe and its use for identification of an intracellular plant membrane K+ channel.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Mi, F. Berkowitz, G. A.
Description	Author Affiliation: Mi F ( Plant Science Department, Cook College, Rutgers State University of New Jersey, New Brunswick 08903, USA.);
Abstract	Polyclonal antibodies were generated against a 9-amino acid, synthetic peptide corresponding to the selectivity filter in the pore region of K(+)-channel proteins. The sequence of amino acids in the ion-conducting pore region of K+ channels is the only highly conserved region of members of this protein family. The objectives of the present work were (i) to determine whether the anti-channel pore peptide antibody was immunoreactive with known K(+)-channel proteins and (ii) to demonstrate the usefulness of the antibody by employing it to identify a newly discovered K(+)-channel protein. Anti-channel pore peptide was immunoreactive with various K(+)-channel subtypes native to a number of different species. Immunoblot analysis demonstrated affinity of the antibody for the drk1, maxi-K, and KAT1 K(+)-channel proteins. Studies also suggested that the anti-channel pore peptide antibody did not immunoreact with membrane proteins other than K+ channels. The anti-channel pore peptide antibody was used to establish the identity of a 62-kDa chloroplast inner envelope polypeptide as a putative component of a K(+)-channel protein. It was concluded that an antibody generated against the conserved pore region/selectivity filter of K+ channels has broad but selective affinity for this class of proteins. This K(+)-channel probe may be a useful tool for identification of K(+)-channel proteins in native membranes.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	8
Volume Number	92
Language	English
Publisher	National Academy of Sciences
Publisher Date	1995-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Chloroplasts Chemistry Intracellular Membranes Plant Proteins Isolation & Purification Potassium Channels Immunology Amino Acid Sequence Animals Antibody Specificity Cells, Cultured Conserved Sequence Immunoblotting Molecular Probes Molecular Sequence Data Peptide Fragments Plants Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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