NDLI: Human acetyl-CoA carboxylase: characterization, molecular cloning, and evidence for two isoforms.

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Human acetyl-CoA carboxylase: characterization, molecular cloning, and evidence for two isoforms.

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Human acetyl-CoA carboxylase: characterization, molecular cloning, and evidence for two isoforms.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Baldini, A. Abu-elheiga, L. Wakil, S. J. Jayakumar, A. Chirala, S. S.
Description	Author Affiliation: Abu-Elheiga L ( Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030, USA.);
Abstract	We have cloned and sequenced the cDNA coding for human HepG2 acetyl-CoA carboxylase (ACC; EC 6.4.1.2). The sequence has an open reading frame of 7038 bp that encode 2346 amino acids (M(r), 264,737). The C-terminal 2.6-kb sequence is very different from that recently reported for human ACC (Ha, J., Daniel, S., Kong, I.-S., Park, C.-K., Tae, H.-J. & Kim, K.-H. [1994] Eur. J. Biochem. 219, 297-306). Northern blot analysis revealed that the ACC mRNA is approximately 10 kb in size and that its level varies among the tissues tested. Evidence is presented to show that the human ACC gene is 200-480 kbp in size and maps to chromosome 17q12. We also provide evidence for the presence of another ACC-like gene with similarly sized mRNA but tissue-specific expression different from that of the ACC gene reported herein. That this second ACC-like gene encodes the 280-kDa carboxylase is not ruled out.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	9
Volume Number	92
Language	English
Publisher	National Academy of Sciences
Publisher Date	1995-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Acetyl-CoA Carboxylase Biosynthesis Genetics Chromosomes, Human, Pair 17 Isoenzymes Chemistry Amino Acid Sequence Animals Carcinoma, Hepatocellular Cell Line Chromosome Mapping Chromosomes, Artificial, Yeast Cloning, Molecular Conserved Sequence DNA, Complementary In Situ Hybridization, Fluorescence Liver Enzymology Liver Neoplasms Molecular Sequence Data Polymerase Chain Reaction Recombinant Proteins Restriction Mapping Saccharomyces Cerevisiae Sequence Homology, Amino Acid Tumor Cells, Cultured Comparative Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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