NDLI: Constitutive production of granulocyte/macrophage colony-stimulating factor by hypodense mononuclear eosinophils developed in vitro from hybrid eosinophil/basophil granulocytes.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1996 Volume: 93 Issue: 26

Year: 1996 Volume: 93 Issue: 25

Year: 1996 Volume: 93 Issue: 24

Year: 1996 Volume: 93 Issue: 23

Year: 1996 Volume: 93 Issue: 22

Year: 1996 Volume: 93 Issue: 21

Year: 1996 Volume: 93 Issue: 20

Year: 1996 Volume: 93 Issue: 19

Year: 1996 Volume: 93 Issue: 18

Year: 1996 Volume: 93 Issue: 17

Year: 1996 Volume: 93 Issue: 16

Year: 1996 Volume: 93 Issue: 15

Year: 1996 Volume: 93 Issue: 14

Year: 1996 Volume: 93 Issue: 13

Year: 1996 Volume: 93 Issue: 12

Year: 1996 Volume: 93 Issue: 11

Year: 1996 Volume: 93 Issue: 10

Year: 1996 Volume: 93 Issue: 9

Year: 1996 Volume: 93 Issue: 8

Year: 1996 Volume: 93 Issue: 7

Year: 1996 Volume: 93 Issue: 6

Unforeseen Americas: the making of New World societies in anthropological perspective.

Colored diffraction catastrophes.

Hyperplane arrangements, interval orders, and trees.

The molecular biology of apoptosis.

The anatomy of T-cell activation and tolerance.

A few autoreactive cells in an autoimmune infiltrate control a vast population of nonspecific cells: a tale of smart bombs and the infantry.

Viruses, cytokines, antigens, and autoimmunity.

Checkpoints in the progression of autoimmune disease: lessons from diabetes models.

Balancing immunity and tolerance: deleting and tuning lymphocyte repertoires.

gamma/delta and other unconventional T lymphocytes: what do they see and what do they do?

A defect in glycosylphosphatidylinositol (GPI) transamidase activity in mutant K cells is responsible for their inability to display GPI surface proteins.

The role of surface loops (residues 204-216 and 627-646) in the motor function of the myosin head.

Temperature effects in hydrophobic interaction chromatography.

Human protein Sam68 relocalization and interaction with poliovirus RNA polymerase in infected cells.

Low-calcium-induced enhancement of chemical synaptic transmission from photoreceptors to horizontal cells in the vertebrate retina.

Identification of a second transmembrane protein tyrosine phosphatase, IA-2beta, as an autoantigen in insulin-dependent diabetes mellitus: precursor of the 37-kDa tryptic fragment.

beta-Amyloid toxicity in organotypic hippocampal cultures: protection by EUK-8, a synthetic catalytic free radical scavenger.

COX-2, a synaptically induced enzyme, is expressed by excitatory neurons at postsynaptic sites in rat cerebral cortex.

Induction of cytochrome P4501A1 by 2,3,7,8-tetrachlorodibenzo-p-dioxin or indolo(3,2-b)carbazole is associated with oxidative DNA damage.

Genes encoding the same three subunits of respiratory complex II are present in the mitochondrial DNA of two phylogenetically distant eukaryotes.

Genetic evidence that the RAG1 protein directly participates in V(D)J recombination through substrate recognition.

Cardioprotective effects of 70-kDa heat shock protein in transgenic mice.

Protein-protein interaction: a genetic selection for compensating mutations at the barnase-barstar interface.

Baculovirus-mediated gene transfer into mammalian cells.

Identification of pentosidine as a native structure for advanced glycation end products in beta-2-microglobulin-containing amyloid fibrils in patients with dialysis-related amyloidosis.

Distinct mechanisms underlie activation of hypothalamic neurosecretory neurons and their medullary catecholaminergic afferents in categorically different stress paradigms.

Evidence for distinct signaling mechanisms in two mammalian olfactory sense organs.

Internal ribosomal entry site substitution eliminates neurovirulence in intergeneric poliovirus recombinants.

Immunolocalization of estrogen receptor protein in the mouse blastocyst during normal and delayed implantation.

Mapping striate and extrastriate visual areas in human cerebral cortex.

p140/c-Abl that binds DNA is preferentially phosphorylated at tyrosine residues.

Agrobacterium VirE2 protein mediates nuclear uptake of single-stranded DNA in plant cells.

Down-regulation of pituitary receptors for luteinizing hormone-releasing hormone (LH-RH) in rats by LH-RH antagonist Cetrorelix.

Mice deficient for prion protein exhibit normal neuronal excitability and synaptic transmission in the hippocampus.

GSP-1 genes are linked to the grain hardness locus (Ha) on wheat chromosome 5D.

Transduction of pluripotent human hematopoietic stem cells demonstrated by clonal analysis after engraftment in immune-deficient mice.

Spontaneous avoidance behavior in Drosophila null for calmodulin expression.

A long-range regulatory element of Hoxc8 identified by using the pClasper vector.

Genetic ablation of parietal cells in transgenic mice: a new model for analyzing cell lineage relationships in the gastric mucosa.

Constitutive production of granulocyte/macrophage colony-stimulating factor by hypodense mononuclear eosinophils developed in vitro from hybrid eosinophil/basophil granulocytes.

Nitric oxide-induced p53 accumulation and regulation of inducible nitric oxide synthase expression by wild-type p53.

Pathogenesis of influenza virus-induced pneumonia: involvement of both nitric oxide and oxygen radicals.

The yeast ZRT1 gene encodes the zinc transporter protein of a high-affinity uptake system induced by zinc limitation.

Non-iron porphyrins cause tumbling to blue light by an Escherichia coli mutant defective in hemG.

High yield conversion of doxorubicin to 2-pyrrolinodoxorubicin, an analog 500-1000 times more potent: structure-activity relationship of daunosamine-modified derivatives of doxorubicin.

Promoter regions involved in density-dependent regulation of basic fibroblast growth factor gene expression in human astrocytic cells.

Antisense globin RNA in mouse erythroid tissues: structure, origin, and possible function.

Ligand occupancy of the alpha-V-beta3 integrin is necessary for smooth muscle cells to migrate in response to insulin-like growth factor.

The response regulator SprE controls the stability of RpoS.

Visualization of intermediate and transition-state structures in protein-tyrosine phosphatase catalysis.

CD40-CD40 ligand interactions in experimental allergic encephalomyelitis and multiple sclerosis.

Human immunodeficiency virus type-1 Tat is an integral component of the activated transcription-elongation complex.

Large electrostatic differences in the binding thermodynamics of a cationic peptide to oligomeric and polymeric DNA.

The translational function of nucleotide C1054 in the small subunit rRNA is conserved throughout evolution: genetic evidence in yeast.

Purification of a ligand for the EPH-like receptor HEK using a biosensor-based affinity detection approach.

The role of DT-diaphorase in the maintenance of the reduced antioxidant form of coenzyme Q in membrane systems.

Intracortical and corticothalamic coherency of fast spontaneous oscillations.

Least activation path for protein folding: investigation of staphylococcal nuclease folding by stopped-flow circular dichroism.

Molecular mechanism of transmembrane signaling by the aspartate receptor: a model.

Tumor suppression and apoptosis of human prostate carcinoma mediated by a genetic locus within human chromosome 10pter-q11.

Progression of human breast cancers to the metastatic state is linked to hydroxyl radical-induced DNA damage.

Active control of waves in a cochlear model with subpartitions.

Inhibition of the association of RNA polymerase II with the preinitiation complex by a viral transcriptional repressor.

Vascular endothelial growth factor B, a novel growth factor for endothelial cells.

Multiplex genotype determination at a large number of gene loci.

CD14+ blood monocytes can differentiate into functionally mature CD83+ dendritic cells.

Identification of a virulence locus encoding a second type III secretion system in Salmonella typhimurium.

The PR5K receptor protein kinase from Arabidopsis thaliana is structurally related to a family of plant defense proteins.

Nonlinear control of heart rate variability in human infants.

Year: 1996 Volume: 93 Issue: 5

Year: 1996 Volume: 93 Issue: 4

Year: 1996 Volume: 93 Issue: 3

Year: 1996 Volume: 93 Issue: 2

Year: 1996 Volume: 93 Issue: 1

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Constitutive production of granulocyte/macrophage colony-stimulating factor by hypodense mononuclear eosinophils developed in vitro from hybrid eosinophil/basophil granulocytes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Boyce, J. A. Owen, W. F. Friend, D. Austen, K. F. Gurish, M. F.
Description	Author Affiliation: Boyce JA ( Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA.);
Abstract	We recently described the development in vitro of cells with granules characteristic of eosinophils and basophils (hybrid granulocytes) from normal human cord blood mononuclear cells cultured for 14 days with recombinant human (rh) interleukin (IL)-3, rhIL-5, and a soluble basement membrane, Matrigel. Hybrid granulocytes constitutively produced granulocyte/macrophage colony-stimulating factor (GM-CSF) and rapidly developed into eosinophils after the exogenous cytokines and Matrigel were removed. To characterize the developmental progression of hybrid granulocytes, cells were maintained for an additional 14 days in medium containing rhIL-3, rhIL-5, and Matrigel. After 28 days, 73% +/- 1% (mean +/- SEM; n = 6) of the nonadherent cells were mononuclear eosinophils, 13% +/- 3% were eosinophils with two or more nuclear lobes, 13% +/- 4% were hybrid granulocytes, and 0.2% +/- 0.1% were basophils. More than 90% of the mononuclear eosinophils were hypodense as determined by centrifugation through metrizamide gradients. After an additional 5 days of culture in medium without exogenous cytokines, 65% +/- 3% (n = 5) of the 28-day cells excluded trypan blue. In contrast, 2% +/- 1% of freshly isolated peripheral blood eosinophils survived 5 days of culture without exogenous cytokines (n = 5). Fifty percent conditioned medium from in vitro derived 28-day mononuclear eosinophils and 14-day hybrid granulocytes maintained the survival of 60% +/- 7% and 77% +/- 7%, respectively, of freshly isolated peripheral blood eosinophils for 72 h, compared with 20% +/- 8% survival in medium alone (n = 3). The eosinophil viability-sustaining activity of 50% mononuclear eosinophil-conditioned medium was neutralized with a GM-CSF antibody. A total of 88% of the 28-day cells exhibited immunochemical staining for GM-CSF. Thus, during eosinophilopoiesis, both hybrid eosinophil/basophil intermediates and immature mononuclear eosinophils exhibit autocrine regulation of viability due to constitutive production of GM-CSF.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	6
Volume Number	93
Language	English
Publisher	National Academy of Sciences
Publisher Date	1996-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Eosinophils Metabolism Granulocyte-Macrophage Colony-Stimulating Factor Cell Survival Cells, Cultured Cytology Fetal Blood Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in