NDLI: Random mutagenesis of Thermus aquaticus DNA polymerase I: concordance of immutable sites in vivo with the crystal structure.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1996 Volume: 93 Issue: 26

Year: 1996 Volume: 93 Issue: 25

Year: 1996 Volume: 93 Issue: 24

Year: 1996 Volume: 93 Issue: 23

Year: 1996 Volume: 93 Issue: 22

Year: 1996 Volume: 93 Issue: 21

Year: 1996 Volume: 93 Issue: 20

Year: 1996 Volume: 93 Issue: 19

Year: 1996 Volume: 93 Issue: 18

Different sources of reduced carbon contribute to form three classes of terpenoid emitted by Quercus ilex L. leaves.

A model of self-thinning through local competition.

Long-range repression in the Drosophila embryo.

An intrinsic genetic program for autonomous differentiation of muscle cells in the ascidian embryo.

Modular cis-regulatory organization of developmentally expressed genes: two genes transcribed territorially in the sea urchin embryo, and additional examples.

Coordinated morphogenesis of epithelia during development of the Caenorhabditis elegans uterine-vulval connection.

An experimental system for analyzing response to a morphogen gradient.

The conserved role of Krox-20 in directing Hox gene expression during vertebrate hindbrain segmentation.

Regulation of patched by sonic hedgehog in the developing neural tube.

The genesis of avian neural crest cells: a classic embryonic induction.

A dynamic assembly of diverse transcription factors integrates activation and cell-type information for interleukin 2 gene regulation.

Combinatorial control of muscle development by basic helix-loop-helix and MADS-box transcription factors.

DNA sequence insertion and evolutionary variation in gene regulation.

Genetic and molecular analysis of the gypsy chromatin insulator of Drosophila.

Chromatin structure and gene expression.

An O(N log N) algorithm for shape modeling.

Temperature dependence of the isotope chemistry of the heavy elements.

Mexican papita viroid: putative ancestor of crop viroids.

Proliferation of multipotent hematopoietic cells controlled by a truncated erythropoietin receptor transgene.

Distortion in the spacer region of Pm during activation of middle transcription of phage Mu.

Use of a designed fusion protein dissociates allosteric properties from the dodecameric state of Pseudomonas aeruginosa catabolic ornithine carbamoyltransferase.

The structure of bovine F1-ATPase complexed with the peptide antibiotic efrapeptin.

A thermodynamic coupling mechanism for GroEL-mediated unfolding.

The mode of action and the structure of a herbicide in complex with its target: binding of activated hydantocidin to the feedback regulation site of adenylosuccinate synthetase.

TRAF5, a novel tumor necrosis factor receptor-associated factor family protein, mediates CD40 signaling.

Peroxisome proliferators induce mouse liver stearoyl-CoA desaturase 1 gene expression.

Glutathione-mediated destabilization in vitro of [2Fe-2S] centers in the SoxR regulatory protein.

Primordial emergence of the recombination activating gene 1 (RAG1): sequence of the complete shark gene indicates homology to microbial integrases.

Hepatocyte nuclear factor 6, a transcription factor that contains a novel type of homeodomain and a single cut domain.

Complete replication of an animal virus and maintenance of expression vectors derived from it in Saccharomyces cerevisiae.

A sequential dimerization mechanism for erythropoietin receptor activation.

An RNA topoisomerase.

Secretory granule targeting of atrial natriuretic peptide correlates with its calcium-mediated aggregation.

The yeast GAL11 protein binds to the transcription factor IIE through GAL11 regions essential for its in vivo function.

Unifying theory of hypoxia tolerance: molecular/metabolic defense and rescue mechanisms for surviving oxygen lack.

Inhibition of cytokines and JAK-STAT activation by distinct signaling pathways.

Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein.

Design of artificial sequence-specific DNA bending ligands.

DNA bending by hexamethylene-tethered ammonium ions.

Conformational dependence of electron transfer across de novo designed metalloproteins.

The entry and clearance of Ca2+ at individual presynaptic active zones of hair cells from the bullfrog's sacculus.

Evidence for nonrandom hydrophobicity structures in protein chains.

Three-dimensional cryoelectron microscopy of dimeric kinesin and ncd motor domains on microtubules.

Cross talk between cell death and cell cycle progression: BCL-2 regulates NFAT-mediated activation.

Cytokine-treated human neutrophils contain inducible nitric oxide synthase that produces nitration of ingested bacteria.

Rab4 and cellubrevin define different early endosome populations on the pathway of transferrin receptor recycling.

Defective propagation of signals generated by sympathetic nerve stimulation in the liver of connexin32-deficient mice.

Apoptosis mediated by HIV protease is preceded by cleavage of Bcl-2.

Inhibition of neoplastic development in the liver by hepatocyte growth factor in a transgenic mouse model.

The herpes simplex virus major regulatory protein ICP4 blocks apoptosis induced by the virus or by hyperthermia.

Forced expression of the tumor suppressor adenomatosis polyposis coli protein induces disordered cell migration in the intestinal epithelium.

Altered surfactant function and structure in SP-A gene targeted mice.

Activation of Stat 5b in erythroid progenitors correlates with the ability of ErbB to induce sustained cell proliferation.

Effects of inefficient cleavage of the signal sequence of HIV-1 gp 120 on its association with calnexin, folding, and intracellular transport.

Split invertase polypeptides form functional complexes in the yeast periplasm in vivo.

Forced expression of tropomyosin 2 or 3 in v-Ki-ras-transformed fibroblasts results in distinct phenotypic effects.

Lack of SHPTP1 results in src-family kinase hyperactivation and thymocyte hyperresponsiveness.

In vitro binding of type 1-fimbriated Escherichia coli to uroplakins Ia and Ib: relation to urinary tract infections.

Functional specificity of Hoxa-4 in vertebral patterning lies outside of the homeodomain.

Embryonic mesoderm cells spread in response to platelet-derived growth factor and signaling by phosphatidylinositol 3-kinase.

Endothelin-B receptor is expressed by neural crest cells in the avian embryo.

A common evolutionary origin for mitochondria and hydrogenosomes.

A carbonic anhydrase from the nacreous layer in oyster pearls.

The whn transcription factor encoded by the nude locus contains an evolutionarily conserved and functionally indispensable activation domain.

Long-lasting reduction of inhibitory function and gamma-aminobutyric acid type A receptor subunit mRNA expression in a model of temporal lobe epilepsy.

Random mutagenesis of Thermus aquaticus DNA polymerase I: concordance of immutable sites in vivo with the crystal structure.

Requirement of proliferating cell nuclear antigen in RAD6-dependent postreplicational DNA repair.

Guanidinium-cholesterol cationic lipids: efficient vectors for the transfection of eukaryotic cells.

Sexual orientation in Drosophila is altered by the satori mutation in the sex-determination gene fruitless that encodes a zinc finger protein with a BTB domain.

Vaccinia virus DNA replication: two hundred base pairs of telomeric sequence confer optimal replication efficiency on minichromosome templates.

CD30/TNF receptor-associated factor interaction: NF-kappa B activation and binding specificity.

Disruption of epithelial gamma delta T cell repertoires by mutation of the Syk tyrosine kinase.

Molecular cloning of a human melanoma-associated chondroitin sulfate proteoglycan.

On the role of antigen in maintaining cytotoxic T-cell memory.

Kinetic analysis of peptide loading onto HLA-DR molecules mediated by HLA-DM.

The immunodominant major histocompatibility complex class I-restricted antigen of a murine colon tumor derives from an endogenous retroviral gene product.

Interleukin 2 production, not the pattern of early T-cell antigen receptor-dependent tyrosine phosphorylation, controls anergy induction by both agonists and partial agonists.

Differential inhibition of HIV-1 preintegration complexes and purified integrase protein by small molecules.

Matrilysin is expressed by lipid-laden macrophages at sites of potential rupture in atherosclerotic lesions and localizes to areas of versican deposition, a proteoglycan substrate for the enzyme.

Heightened expression of tumor necrosis factor alpha, interleukin 1 alpha, and glial fibrillary acidic protein in experimental Creutzfeldt-Jakob disease in mice.

Transloading of tumor cells with foreign major histocompatibility complex class I peptide ligand: a novel general strategy for the generation of potent cancer vaccines.

Involvement of integrins alpha v beta 3 and alpha v beta 5 in ocular neovascular diseases.

FHIT gene alterations in head and neck squamous cell carcinomas.

17 beta-estradiol hydroxylation catalyzed by human cytochrome P450 1B1.

Neurodegeneration, myocardial injury, and perinatal death in mitochondrial superoxide dismutase-deficient mice.

In vitro activation of human herpesviruses 6 and 7 from latency.

Irreversibility of cellular aging and neoplastic transformation: a clonal analysis.

Cholesterol homeostasis in human brain: evidence for an age-dependent flux of 24S-hydroxycholesterol from the brain into the circulation.

The hybrid PAX3-FKHR fusion protein of alveolar rhabdomyosarcoma transforms fibroblasts in culture.

A candidate live inactivatable attenuated vaccine for AIDS.

On the etiology of Crohn disease.

Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.

Type 1 fimbrial expression enhances Escherichia coli virulence for the urinary tract.

Salmonella typhimurium invasion induces apoptosis in infected macrophages.

The alginate regulator AlgR and an associated sensor FimS are required for twitching motility in Pseudomonas aeruginosa.

Presence of mRNA for glutamic acid decarboxylase in both excitatory and inhibitory neurons.

Fibroblast growth factor (FGF) homologous factors: new members of the FGF family implicated in nervous system development.

Olfactory marker protein (OMP) gene deletion causes altered physiological activity of olfactory sensory neurons.

Ablation of suprachiasmatic nucleus alters timing of hibernation in ground squirrels.

Subthreshold facilitation and suppression in primary visual cortex revealed by intrinsic signal imaging.

Expression of Drosophila mushroom body mutations in alternative genetic backgrounds: a case study of the mushroom body miniature gene (mbm).

Identification of potential target genes for the neuron-restrictive silencer factor.

Induction of myelination in the central nervous system by electrical activity.

Bcl-2 potentiates the maximal calcium uptake capacity of neural cell mitochondria.

Subcellular localization of gamma-aminobutyric acid type A receptors is determined by receptor beta subunits.

Quantal release at a neuronal nicotinic synapse from rat adrenal gland.

Elevation of intracellular calcium by muscarinic receptor activation induces a block of voltage-activated rat ether-à-go-go channels in a stably transfected cell line.

Low lead levels stunt neuronal growth in a reversible manner.

Pattern and inhibition-dependent invasion of pyramidal cell dendrites by fast spikes in the hippocampus in vivo.

Preserved neuron number in the hippocampus of aged rats with spatial learning deficits.

Enhancement of sensorimotor connections by conditioning-related stimulation in Aplysia depends upon postsynaptic Ca2+.

Glyceraldehyde-3-phosphate dehydrogenase antisense oligodeoxynucleotides protect against cytosine arabinonucleoside-induced apoptosis in cultured cerebellar neurons.

The pfmdr1 gene of Plasmodium falciparum confers cellular resistance to antimalarial drugs in yeast cells. (Journal: Proceedings of the National Academy of Sciences of the United States of America Volume: 93 Issue: 18)

Isolated rat hepatocytes can signal to other hepatocytes and bile duct cells by release of nucleotides.

Receptor-specific in vivo desensitization by the G protein-coupled receptor kinase-5 in transgenic mice.

Chronic control of high blood pressure in the spontaneously hypertensive rat by delivery of angiotensin type 1 receptor antisense.

Environmental stress sensitivity of an ascorbic acid-deficient Arabidopsis mutant.

Stable transfer of intact high molecular weight DNA into plant chromosomes.

Schizophrenia and cognitive dysmetria: a positron-emission tomography study of dysfunctional prefrontal-thalamic-cerebellar circuitry.

Biology of Developmental Transcription Control. Conference proceedings. Irvine, California, October 26-28, 1995.

Year: 1996 Volume: 93 Issue: 17

Year: 1996 Volume: 93 Issue: 16

Year: 1996 Volume: 93 Issue: 15

Year: 1996 Volume: 93 Issue: 14

Year: 1996 Volume: 93 Issue: 13

Year: 1996 Volume: 93 Issue: 12

Year: 1996 Volume: 93 Issue: 11

Year: 1996 Volume: 93 Issue: 10

Year: 1996 Volume: 93 Issue: 9

Year: 1996 Volume: 93 Issue: 8

Year: 1996 Volume: 93 Issue: 7

Year: 1996 Volume: 93 Issue: 6

Year: 1996 Volume: 93 Issue: 5

Year: 1996 Volume: 93 Issue: 4

Year: 1996 Volume: 93 Issue: 3

Year: 1996 Volume: 93 Issue: 2

Year: 1996 Volume: 93 Issue: 1

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Random mutagenesis of Thermus aquaticus DNA polymerase I: concordance of immutable sites in vivo with the crystal structure.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Suzuki, M. Baskin, D. Hood, L. Loeb, L. A.
Description	Author Affiliation: Suzuki M ( Joseph Gottstein Memorial Cancer Research Laboratory, Department of Pathology, Seattle, WA, USA.);
Abstract	Expression of Thermus aquaticus (Taq) DNA polymerase I (pol I) in Escherichia, coli complements the growth defect caused by a temperature-sensitive mutation in the host pol I. We replaced the nucleotide sequence encoding amino acids 659-671 of the O-helix of Taq DNA pol I, corresponding to the substrate binding site, with an oligonucleotide containing random nucleotides. Functional Taq pol I mutants were selected based on colony formation at the nonpermissive temperature. By using a library with 9% random substitutions at each of 39 positions, we identified 61 active Taq pol I mutants, each of which contained from one to four amino acid substitutions. Some amino acids, such as alanine-661 and threonine-664, were tolerant of several or even many diverse replacements. In contrast, no replacements or only conservative replacements were identified at arginine-659, lysine-663, and tyrosine-671. By using a library with totally random nucleotides at five different codons (arginine-659, arginine-660, lysine-663, phenylalanine-667, and glycine-668), we confirmed that arginine-659 and lysine-663 were immutable, and observed that only tyrosine substituted for phenylalanine-667. The two immutable residues and the two residues that tolerate only highly conservative replacements lie on the side of O-helix facing the incoming deoxynucleoside triphosphate, as determined by x-ray analysis. Thus, we offer a new approach to assess concordance of the active conformation of an enzyme, as interpreted from the crystal structure, with the active conformation inferred from in vivo function.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	18
Volume Number	93
Language	English
Publisher	National Academy of Sciences
Publisher Date	1996-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	DNA Polymerase I Genetics Thermus Enzymology Amino Acid Sequence Arginine Codon Crystallography, X-Ray Chemistry Escherichia Coli Glycine Lysine Molecular Sequence Data Mutagenesis, Site-Directed Phenylalanine Plasmids Metabolism Protein Conformation Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in